Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E2P79_RS03185 Genome accession   NZ_CP039611
Coordinates   658065..659099 (+) Length   344 a.a.
NCBI ID   WP_050667366.1    Uniprot ID   -
Organism   Aeromonas schubertii strain LF1708     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 653065..664099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2P79_RS03145 (E2P79_03145) rpoS 653144..654127 (+) 984 WP_060584075.1 RNA polymerase sigma factor RpoS -
  E2P79_RS03150 (E2P79_03150) hupA 654344..654616 (+) 273 WP_005351739.1 nucleoid-associated protein HU-alpha -
  E2P79_RS03155 (E2P79_03155) - 654724..655140 (+) 417 WP_050667360.1 hypothetical protein -
  E2P79_RS03160 (E2P79_03160) - 655192..655611 (-) 420 WP_050667361.1 DUF4426 domain-containing protein -
  E2P79_RS03165 (E2P79_03165) yggU 655640..655930 (-) 291 WP_136612846.1 DUF167 family protein YggU -
  E2P79_RS03170 (E2P79_03170) - 655930..656481 (-) 552 WP_050667363.1 YggT family protein -
  E2P79_RS03175 (E2P79_03175) proC 656491..657312 (-) 822 WP_136612847.1 pyrroline-5-carboxylate reductase -
  E2P79_RS03180 (E2P79_03180) - 657324..658025 (-) 702 WP_136612848.1 YggS family pyridoxal phosphate-dependent enzyme -
  E2P79_RS03185 (E2P79_03185) pilT 658065..659099 (+) 1035 WP_050667366.1 type IVa pilus ATPase TapT Machinery gene
  E2P79_RS03190 (E2P79_03190) pilU 659108..660226 (+) 1119 WP_136612849.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E2P79_RS03195 (E2P79_03195) yaaA 660241..661020 (+) 780 WP_136612850.1 peroxide stress protein YaaA -
  E2P79_RS03200 (E2P79_03200) srmB 661164..662393 (-) 1230 WP_050667369.1 ATP-dependent RNA helicase SrmB -
  E2P79_RS03205 (E2P79_03205) - 662508..663215 (+) 708 WP_050667370.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38354.07 Da        Isoelectric Point: 6.6938

>NTDB_id=360076 E2P79_RS03185 WP_050667366.1 658065..659099(+) (pilT) [Aeromonas schubertii strain LF1708]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEMRKINLPVLEHREVHALIYDIMNDQQRKELEENYEVDFSFEIPGLAR
FRVNAFQQSRGSGAVFRTIPSTVLSLDELDAPEVFRKIAEYPRGLVLVTGPTGSGKSTTLAAMINYINESFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMIGIPAVRNLIREDKVPQLYSVIQTGMTHGMQTMDQCL
KQLVGRGVVTQQDARGKAADPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=360076 E2P79_RS03185 WP_050667366.1 658065..659099(+) (pilT) [Aeromonas schubertii strain LF1708]
ATGGATATCACAGAGTTATTGGCCTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCTGCCGGGGTGCCACCCAT
GATCCGGGTCGACGGGGAGATGCGCAAGATCAATCTGCCCGTCCTGGAGCACAGGGAGGTTCACGCCCTCATCTATGACA
TCATGAACGATCAGCAGCGCAAGGAGCTTGAAGAGAATTACGAGGTCGACTTCTCCTTCGAGATCCCCGGGTTGGCGCGC
TTTCGTGTCAACGCCTTCCAGCAGTCTCGTGGCTCGGGAGCCGTCTTCCGGACCATCCCCAGTACAGTATTGAGTCTCGA
TGAGCTCGATGCTCCCGAGGTATTTCGCAAGATCGCCGAATATCCCCGTGGTCTGGTACTGGTGACGGGGCCAACCGGCT
CGGGCAAGTCGACGACGCTGGCCGCCATGATCAACTACATCAACGAGTCCTTTCATCATCACATCCTCACCATCGAGGAT
CCCATCGAGTTCGTGCATGAGAACAAACGCTGTCTGGTGAACCAGCGCGAGGTGCACCGGGATACCAAGAGCTTCAGCAA
TGCCCTGCGCTCGGCCCTGCGTGAAGACCCCGACATCATACTGGTCGGTGAGATGCGGGATCTCGAAACCATTCGGCTGG
CGATGACGGCGGCCGAGACCGGTCACCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATAGACCGTATC
ATCGACGTCTTCCCCGGGGCCGAGAAGGACATGGTGCGCTCCATGTTGTCCGAATCCCTGCGGGCGGTTATCTCCCAGAC
CCTGCTCAAACGTATCGGCGGTGGTCGGGTGGCCGCACACGAGATCATGATAGGTATCCCGGCGGTGCGAAACCTGATCC
GGGAAGACAAGGTGCCCCAGCTCTACTCTGTGATTCAGACCGGGATGACACATGGCATGCAGACCATGGATCAGTGCCTC
AAGCAGTTGGTCGGGCGCGGCGTGGTTACCCAGCAGGATGCCAGGGGCAAGGCCGCCGATCCCAACAGTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.163

100

0.762
  pilT Acinetobacter baylyi ADP1

76.163

100

0.762
  pilT Acinetobacter baumannii D1279779

75.581

100

0.756
  pilT Acinetobacter baumannii strain A118

75.581

100

0.756
  pilT Acinetobacter nosocomialis M2

75.581

100

0.756
  pilT Pseudomonas stutzeri DSM 10701

75.581

100

0.756
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

74.481

97.965

0.73
  pilT Vibrio cholerae strain A1552

74.481

97.965

0.73
  pilT Legionella pneumophila strain Lp02

71.893

98.256

0.706
  pilT Legionella pneumophila strain ERS1305867

71.893

98.256

0.706
  pilT Neisseria meningitidis 8013

69.275

100

0.695
  pilT Neisseria gonorrhoeae MS11

68.986

100

0.692
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.632

99.419

0.523
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

41.739

100

0.419
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407

Multiple sequence alignment