Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   E2P79_RS03190 Genome accession   NZ_CP039611
Coordinates   659108..660226 (+) Length   372 a.a.
NCBI ID   WP_136612849.1    Uniprot ID   -
Organism   Aeromonas schubertii strain LF1708     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 654108..665226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2P79_RS03150 (E2P79_03150) hupA 654344..654616 (+) 273 WP_005351739.1 nucleoid-associated protein HU-alpha -
  E2P79_RS03155 (E2P79_03155) - 654724..655140 (+) 417 WP_050667360.1 hypothetical protein -
  E2P79_RS03160 (E2P79_03160) - 655192..655611 (-) 420 WP_050667361.1 DUF4426 domain-containing protein -
  E2P79_RS03165 (E2P79_03165) yggU 655640..655930 (-) 291 WP_136612846.1 DUF167 family protein YggU -
  E2P79_RS03170 (E2P79_03170) - 655930..656481 (-) 552 WP_050667363.1 YggT family protein -
  E2P79_RS03175 (E2P79_03175) proC 656491..657312 (-) 822 WP_136612847.1 pyrroline-5-carboxylate reductase -
  E2P79_RS03180 (E2P79_03180) - 657324..658025 (-) 702 WP_136612848.1 YggS family pyridoxal phosphate-dependent enzyme -
  E2P79_RS03185 (E2P79_03185) pilT 658065..659099 (+) 1035 WP_050667366.1 type IVa pilus ATPase TapT Machinery gene
  E2P79_RS03190 (E2P79_03190) pilU 659108..660226 (+) 1119 WP_136612849.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E2P79_RS03195 (E2P79_03195) yaaA 660241..661020 (+) 780 WP_136612850.1 peroxide stress protein YaaA -
  E2P79_RS03200 (E2P79_03200) srmB 661164..662393 (-) 1230 WP_050667369.1 ATP-dependent RNA helicase SrmB -
  E2P79_RS03205 (E2P79_03205) - 662508..663215 (+) 708 WP_050667370.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  E2P79_RS03210 (E2P79_03210) brnQ 663451..664713 (+) 1263 WP_050667398.1 branched-chain amino acid transport system II carrier protein -
  E2P79_RS03215 (E2P79_03215) - 664823..665059 (-) 237 WP_050667371.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41258.55 Da        Isoelectric Point: 6.3509

>NTDB_id=360077 E2P79_RS03190 WP_136612849.1 659108..660226(+) (pilU) [Aeromonas schubertii strain LF1708]
MSMDAWLGELVARRGSDLFVTTGMAPTVKQDGRFVALGDTPLSAKEVEEAMALIMDEESRHLFAQTREANFAIQRPGLGR
FRVSTFLQLAQPGMVLRRIETRIPTFEELKLPPVLREVVMAKRGLILFVGATGAGKSTTQAAMIGYRNQQSDGHILTVED
PVEFVHHHGRSIVTQREVGLDTPSFDSALRSALRQAPDLILIGEIRTQETMEFAIQFAETGHICMATLHANNADQAIERI
MHLMPEGRHRQLRYDLAFNLKAIVAQQLLPLKSGRGRGAAFEILLNTPLIADIIRKGEVDRLKDVMGRSREQGMVTFDQS
LFDLYMGGQIDMETALAHADSVNDLRLMIKLKGGEKLGSGSLDNVTLQPDME

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=360077 E2P79_RS03190 WP_136612849.1 659108..660226(+) (pilU) [Aeromonas schubertii strain LF1708]
ATGAGCATGGATGCCTGGTTGGGCGAGCTGGTGGCGAGGCGTGGTTCGGATCTCTTCGTCACGACTGGCATGGCCCCTAC
CGTGAAGCAGGACGGGCGCTTCGTCGCCCTCGGCGATACGCCCTTGTCGGCAAAAGAGGTTGAAGAGGCGATGGCCCTCA
TCATGGATGAGGAGAGCAGACACCTCTTTGCACAGACCAGGGAGGCCAACTTCGCCATACAGAGGCCGGGTCTCGGTCGT
TTCCGGGTCAGTACTTTCCTGCAACTGGCCCAGCCTGGCATGGTGCTGCGTCGCATCGAGACCCGGATCCCCACCTTCGA
AGAGCTCAAGCTGCCACCTGTGCTCAGGGAGGTCGTGATGGCCAAGCGGGGGCTGATCCTCTTCGTCGGTGCCACCGGTG
CCGGTAAGTCGACGACCCAGGCGGCCATGATCGGCTATCGCAATCAGCAGAGTGATGGCCACATCCTGACGGTCGAAGAT
CCGGTGGAATTTGTGCATCACCACGGGCGCAGCATCGTGACCCAGCGAGAGGTGGGGCTGGATACGCCCTCTTTCGATTC
GGCCCTGCGCAGTGCCCTGCGTCAGGCGCCGGACCTTATCCTGATCGGCGAGATCCGTACCCAGGAGACCATGGAGTTTG
CCATCCAGTTTGCCGAGACGGGCCATATCTGCATGGCCACTCTGCACGCCAACAATGCTGACCAGGCGATCGAACGGATC
ATGCATCTGATGCCGGAGGGGCGCCACCGCCAGCTGCGCTACGATCTGGCGTTCAATCTGAAGGCCATCGTCGCCCAGCA
GTTGCTGCCCCTCAAGAGTGGTCGTGGGCGCGGTGCTGCGTTCGAGATCCTGCTCAACACGCCCCTGATTGCCGACATCA
TCCGTAAAGGAGAGGTGGACAGACTCAAGGATGTGATGGGGCGCTCCCGCGAGCAGGGGATGGTGACCTTCGATCAATCC
CTCTTCGATCTCTACATGGGGGGACAGATCGACATGGAGACGGCCCTGGCCCATGCGGACTCGGTGAACGATCTCCGGTT
GATGATCAAGCTGAAGGGGGGTGAGAAACTTGGCTCCGGCTCTCTGGATAATGTTACCCTGCAGCCCGATATGGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

58.333

100

0.583
  pilU Pseudomonas stutzeri DSM 10701

57.418

97.849

0.562
  pilU Acinetobacter baylyi ADP1

50.938

100

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.11

85.215

0.384
  pilT Acinetobacter baylyi ADP1

40.469

91.667

0.371
  pilT Legionella pneumophila strain Lp02

40.597

90.054

0.366
  pilT Legionella pneumophila strain ERS1305867

40.597

90.054

0.366

Multiple sequence alignment