Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VB87_RS11050 Genome accession   NC_013722
Coordinates   2547225..2548262 (-) Length   345 a.a.
NCBI ID   WP_012916643.1    Uniprot ID   D2UEP7
Organism   Xanthomonas albilineans GPE PC73     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2542225..2553262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VB87_RS11025 (XALC_2155) - 2543054..2544001 (-) 948 WP_012916638.1 aspartate carbamoyltransferase catalytic subunit -
  VB87_RS11030 (XALC_2156) ruvX 2544020..2544517 (-) 498 WP_012916639.1 Holliday junction resolvase RuvX -
  VB87_RS11035 (XALC_2157) - 2544510..2545076 (-) 567 WP_012916640.1 YqgE/AlgH family protein -
  VB87_RS11040 (XALC_2158) - 2545392..2545931 (+) 540 WP_012916641.1 DNA-3-methyladenine glycosylase I -
  VB87_RS11045 (XALC_2159) pilU 2545970..2547100 (-) 1131 WP_012916642.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VB87_RS11050 (XALC_2160) pilT 2547225..2548262 (-) 1038 WP_012916643.1 type IV pilus twitching motility protein PilT Machinery gene
  VB87_RS11055 (XALC_2161) - 2548365..2549039 (+) 675 WP_080928215.1 YggS family pyridoxal phosphate-dependent enzyme -
  VB87_RS11060 (XALC_2162) - 2549305..2550345 (+) 1041 WP_230598079.1 hypothetical protein -
  VB87_RS11065 (XALC_2163) proC 2550495..2551337 (+) 843 WP_012916646.1 pyrroline-5-carboxylate reductase -
  VB87_RS11070 (XALC_2164) pdxH 2551545..2552144 (-) 600 WP_012916647.1 pyridoxamine 5'-phosphate oxidase -
  VB87_RS11075 (XALC_2165) - 2552266..2552808 (+) 543 WP_012916648.1 shikimate kinase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38525.20 Da        Isoelectric Point: 6.5931

>NTDB_id=35981 VB87_RS11050 WP_012916643.1 2547225..2548262(-) (pilT) [Xanthomonas albilineans GPE PC73]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDRQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTLGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRAAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQSGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=35981 VB87_RS11050 WP_012916643.1 2547225..2548262(-) (pilT) [Xanthomonas albilineans GPE PC73]
ATGGATATTGCGGAACTATTGGCGTTTTCGGTCAAGAACAAGGCGTCCGACCTGCACCTGTCCGCGGGTTTGCCGCCGAT
GATCCGGGTCGACGGCGACGTGCGCCGCATCAACATCCCGGCGCTGGACCACAAGCAAGTCCATGCGTTGGTCTACGACA
TCATGTCCGACCGGCAACGCCGCGATTACGAGGAATTCCTCGAGGTCGACTTTTCGTTCGAGATCCCCAGTCTGGCGCGC
TTTCGCGTTAATGCGTTCAACCAGAACCGTGGTGCTGGCGCGGTGTTTCGTACCATTCCTTCGGAAGTGCTGACCCTGGA
AGACCTGGGCTGCCCGCCGATCTTTCGCCAATTGATCGACCAGCCGCAGGGGCTGATCCTAGTGACCGGGCCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGGCATGATCGATTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAATCAGCGCGAGGTGCACCGCGATACTCTTGGGTTCAACGA
AGCGCTGCGTTCGGCCTTGCGCGAAGATCCCGATATCATTCTAGTCGGCGAGTTGCGCGACCTGGAGACCATCCGTCTGG
CGCTGACCGCCGCCGAAACCGGCCATCTGGTGTTCGGCACGTTGCATACCAGTTCAGCGGCCAAGACCATCGATCGCATC
ATCGACGTCTTTCCCGCTGGCGAAAAGCCGATGGTGCGTTCGATGCTGTCCGAATCGCTGCGTGCGGTGATTTCCCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCGCCGCCGCCTGGGAAATCATGGTCGGCACCCCGGCGATTCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACTGGCCAGCAGAGCGGCATGCAGACCTTGGATCAGCATCTG
CAAGACCTGGTCAAGCGTAGTCTGATCACCCGCAACCAAGCGCGCGAGTACGCCAAGGACAAGCGGATCTTCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D2UEP7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

79.351

98.261

0.78
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

66.569

98.841

0.658
  pilT Neisseria gonorrhoeae MS11

66.276

98.841

0.655
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.504
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment