Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VB87_RS11045 Genome accession   NC_013722
Coordinates   2545970..2547100 (-) Length   376 a.a.
NCBI ID   WP_012916642.1    Uniprot ID   D2UEP6
Organism   Xanthomonas albilineans GPE PC73     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2540970..2552100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VB87_RS11025 (XALC_2155) - 2543054..2544001 (-) 948 WP_012916638.1 aspartate carbamoyltransferase catalytic subunit -
  VB87_RS11030 (XALC_2156) ruvX 2544020..2544517 (-) 498 WP_012916639.1 Holliday junction resolvase RuvX -
  VB87_RS11035 (XALC_2157) - 2544510..2545076 (-) 567 WP_012916640.1 YqgE/AlgH family protein -
  VB87_RS11040 (XALC_2158) - 2545392..2545931 (+) 540 WP_012916641.1 DNA-3-methyladenine glycosylase I -
  VB87_RS11045 (XALC_2159) pilU 2545970..2547100 (-) 1131 WP_012916642.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VB87_RS11050 (XALC_2160) pilT 2547225..2548262 (-) 1038 WP_012916643.1 type IV pilus twitching motility protein PilT Machinery gene
  VB87_RS11055 (XALC_2161) - 2548365..2549039 (+) 675 WP_080928215.1 YggS family pyridoxal phosphate-dependent enzyme -
  VB87_RS11060 (XALC_2162) - 2549305..2550345 (+) 1041 WP_230598079.1 hypothetical protein -
  VB87_RS11065 (XALC_2163) proC 2550495..2551337 (+) 843 WP_012916646.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41941.21 Da        Isoelectric Point: 6.8813

>NTDB_id=35980 VB87_RS11045 WP_012916642.1 2545970..2547100(-) (pilU) [Xanthomonas albilineans GPE PC73]
MSTTDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTPQQSRDMVLNVMTPGQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPSVDELSLPPVIKTLAMTKRGIIIFVGATGAGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRVINFFPEDRRSQLLMELSLNLRGVVAQQLIPTPDGKSRRVAMEIMLGTPLIQDYIREGEIHKLKDVMKESINLGMRTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=35980 VB87_RS11045 WP_012916642.1 2545970..2547100(-) (pilU) [Xanthomonas albilineans GPE PC73]
ATGAGTACCACCGACTTCACCTCGTTCCTGAAACTGATGGCGCACCAAAAAGCGTCGGATCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATCACGCAGACGCCGCTGACCCCGCAGCAGAGCCGCGACATGG
TGTTGAACGTGATGACGCCTGGGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTTGGCCGCTTCCGCGTCAGTTGCTTCTACCAGCGTAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGCATTCC
CAGCGTGGACGAACTGAGCTTGCCGCCGGTGATTAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTTGGTG
CGACCGGGGCCGGTAAATCGACCTCACTGGCGGCGATGATCGGTTATCGCAACCAGAACTCCACCGGACACATCATCACC
ATCGAAGATCCGATTGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGGATCGACACCGACAG
CTGGGAAAACGCATTGAAAAATACCCTGCGTCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCCATTTCCTTCGCCGAAACCGGCCACCTGGTGCTGTGTACTTTGCATGCCAACAACGCCAACCAGGCGATG
GATCGCGTCATCAACTTCTTCCCTGAAGACCGTCGCAGTCAATTGTTGATGGAACTATCGCTGAACCTGCGGGGCGTGGT
CGCCCAGCAATTGATCCCGACTCCGGATGGCAAGAGCCGTCGGGTGGCGATGGAAATCATGCTCGGGACTCCGTTGATCC
AGGATTACATCCGCGAAGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAGTCCATCAATCTGGGCATGCGAACCTTC
GATCAGAGCTTGTTCGAGTTGTATCAGGCTGGCGAGATCAGCTACGAGGACGCGCTGCGTCACGCCGATTCGCAGAACGA
AGTGCGCTTGCGCATCAAGTTGGCCCAGGGCGGCGACGCCAGAACCCTGGCGCAGGGCTTGGACGGGGTCGAGATCGCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D2UEP6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.574

97.34

0.638
  pilU Acinetobacter baylyi ADP1

62.921

94.681

0.596
  pilU Vibrio cholerae strain A1552

52.394

94.415

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.857

93.085

0.38
  pilT Acinetobacter baumannii strain A118

40.233

91.223

0.367
  pilT Acinetobacter nosocomialis M2

40.233

91.223

0.367
  pilT Acinetobacter baumannii D1279779

40.233

91.223

0.367
  pilT Pseudomonas aeruginosa PAK

40.828

89.894

0.367

Multiple sequence alignment