Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E8E00_RS01790 Genome accession   NZ_CP039516
Coordinates   399264..400289 (+) Length   341 a.a.
NCBI ID   WP_069591174.1    Uniprot ID   A0A2A4GI28
Organism   Salinivibrio sp. YCSC6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 394264..405289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8E00_RS01755 (E8E00_01755) hemW 394482..395639 (-) 1158 WP_096629940.1 radical SAM family heme chaperone HemW -
  E8E00_RS01760 (E8E00_01760) rdgB 395642..396238 (-) 597 WP_096629937.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  E8E00_RS01765 (E8E00_01765) - 396267..396707 (-) 441 WP_069591168.1 DUF4426 domain-containing protein -
  E8E00_RS01770 (E8E00_01770) - 396769..397065 (-) 297 WP_096629934.1 DUF167 family protein -
  E8E00_RS01775 (E8E00_01775) - 397065..397619 (-) 555 WP_077677951.1 YggT family protein -
  E8E00_RS01780 (E8E00_01780) proC 397636..398454 (-) 819 WP_077457418.1 pyrroline-5-carboxylate reductase -
  E8E00_RS01785 (E8E00_01785) - 398539..399240 (-) 702 WP_096629931.1 YggS family pyridoxal phosphate-dependent enzyme -
  E8E00_RS01790 (E8E00_01790) pilT 399264..400289 (+) 1026 WP_069591174.1 type IV pilus twitching motility protein PilT Machinery gene
  E8E00_RS01795 (E8E00_01795) pilU 400298..401416 (+) 1119 WP_096629928.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E8E00_RS01800 (E8E00_01800) ruvX 401413..401841 (-) 429 WP_069591176.1 Holliday junction resolvase RuvX -
  E8E00_RS01805 (E8E00_01805) - 401838..402404 (-) 567 WP_069591178.1 YqgE/AlgH family protein -
  E8E00_RS17105 gshB 402496..403440 (-) 945 WP_096629925.1 glutathione synthase -
  E8E00_RS17110 rsmE 403462..404193 (-) 732 WP_069591180.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  E8E00_RS01815 (E8E00_01815) - 404308..404811 (-) 504 WP_096629922.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37332.72 Da        Isoelectric Point: 6.5569

>NTDB_id=359428 E8E00_RS01790 WP_069591174.1 399264..400289(+) (pilT) [Salinivibrio sp. YCSC6]
MDITELLAFSVKHNASDLHLSAGVAPMVRVDGDVRKLSLPALSHQEVNQLVLDVMNDNQQREFQAQLEVDFSFHMQSVGR
FRVNAFHQQHGCAAVFRVIPETVPSLADLHAPEIFADFARLSNGLVLVTGPTGSGKSTTLAAMVDHINRHAPKHILTIED
PIEFIHHPQQSLINQREVKRDTLSFQQALRSALREDPDVILVGELRDLDTISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGSDKPLVRSMLSASLRAVVSQQLIKTAQGGRVAAHEIMIASPAIRNLIREDKVAQMVSMIQTGSNQGMQTMEQAL
ADLVGQGTIAPEQRDRVLRAL

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=359428 E8E00_RS01790 WP_069591174.1 399264..400289(+) (pilT) [Salinivibrio sp. YCSC6]
ATGGATATCACTGAGCTATTGGCCTTTAGTGTAAAACATAATGCGTCAGATCTACATCTTTCCGCGGGAGTGGCGCCTAT
GGTGCGGGTAGATGGCGATGTGCGCAAGTTGAGTTTGCCAGCGTTGAGCCATCAAGAGGTCAATCAATTGGTCTTGGATG
TGATGAATGATAACCAGCAGCGTGAATTTCAAGCGCAGTTGGAAGTGGATTTTTCATTTCATATGCAGTCTGTGGGACGT
TTTCGGGTTAATGCATTTCATCAGCAACACGGCTGCGCGGCGGTATTTCGGGTGATCCCCGAGACAGTCCCTAGTTTGGC
GGATCTCCATGCCCCCGAGATTTTTGCGGATTTTGCTCGGCTCTCAAATGGATTAGTGTTGGTCACCGGCCCGACGGGCT
CGGGGAAGTCCACCACATTGGCGGCCATGGTTGATCACATTAACCGCCACGCACCTAAGCATATTCTTACCATCGAAGAC
CCGATTGAGTTTATCCATCACCCGCAACAAAGTTTGATTAATCAGCGTGAAGTGAAGCGCGATACCTTATCGTTTCAACA
AGCGCTGCGTTCGGCGTTGCGTGAAGATCCCGATGTGATTTTGGTAGGAGAGCTGCGAGATCTCGATACCATTAGTTTGG
CGTTAACGGCGGCGGAAACTGGACATCTGGTATTCGGCACCTTGCATACCTCAAGCGCCGCAAAAACAGTCGACCGTATT
ATTGATGTCTTTCCCGGCAGTGATAAGCCGCTGGTCCGCTCGATGCTTTCAGCCTCACTGCGGGCGGTTGTGTCTCAGCA
GCTGATAAAAACAGCGCAAGGTGGGCGCGTGGCCGCGCATGAAATCATGATCGCAAGCCCAGCGATTCGTAACCTCATCC
GTGAGGATAAGGTGGCGCAAATGGTGTCGATGATTCAAACCGGCTCCAATCAAGGGATGCAAACCATGGAGCAAGCACTG
GCCGATTTAGTCGGACAAGGGACGATCGCGCCTGAACAACGCGATCGCGTATTACGTGCGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A4GI28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.171

97.654

0.695
  pilT Vibrio cholerae strain A1552

71.171

97.654

0.695
  pilT Acinetobacter baumannii D1279779

66.566

97.361

0.648
  pilT Acinetobacter baumannii strain A118

66.566

97.361

0.648
  pilT Acinetobacter nosocomialis M2

66.566

97.361

0.648
  pilT Neisseria meningitidis 8013

65.766

97.654

0.642
  pilT Neisseria gonorrhoeae MS11

64.985

98.827

0.642
  pilT Acinetobacter baylyi ADP1

66.565

96.481

0.642
  pilT Pseudomonas aeruginosa PAK

65.465

97.654

0.639
  pilT Pseudomonas stutzeri DSM 10701

65.455

96.774

0.633
  pilT Legionella pneumophila strain Lp02

64.242

96.774

0.622
  pilT Legionella pneumophila strain ERS1305867

64.242

96.774

0.622
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.778

95.015

0.501
  pilU Pseudomonas stutzeri DSM 10701

41.742

97.654

0.408
  pilU Acinetobacter baylyi ADP1

41.566

97.361

0.405
  pilU Vibrio cholerae strain A1552

40.615

95.308

0.387

Multiple sequence alignment