Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   E8E00_RS01795 Genome accession   NZ_CP039516
Coordinates   400298..401416 (+) Length   372 a.a.
NCBI ID   WP_096629928.1    Uniprot ID   A0A2A4GJD3
Organism   Salinivibrio sp. YCSC6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 395298..406416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8E00_RS01760 (E8E00_01760) rdgB 395642..396238 (-) 597 WP_096629937.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  E8E00_RS01765 (E8E00_01765) - 396267..396707 (-) 441 WP_069591168.1 DUF4426 domain-containing protein -
  E8E00_RS01770 (E8E00_01770) - 396769..397065 (-) 297 WP_096629934.1 DUF167 family protein -
  E8E00_RS01775 (E8E00_01775) - 397065..397619 (-) 555 WP_077677951.1 YggT family protein -
  E8E00_RS01780 (E8E00_01780) proC 397636..398454 (-) 819 WP_077457418.1 pyrroline-5-carboxylate reductase -
  E8E00_RS01785 (E8E00_01785) - 398539..399240 (-) 702 WP_096629931.1 YggS family pyridoxal phosphate-dependent enzyme -
  E8E00_RS01790 (E8E00_01790) pilT 399264..400289 (+) 1026 WP_069591174.1 type IV pilus twitching motility protein PilT Machinery gene
  E8E00_RS01795 (E8E00_01795) pilU 400298..401416 (+) 1119 WP_096629928.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E8E00_RS01800 (E8E00_01800) ruvX 401413..401841 (-) 429 WP_069591176.1 Holliday junction resolvase RuvX -
  E8E00_RS01805 (E8E00_01805) - 401838..402404 (-) 567 WP_069591178.1 YqgE/AlgH family protein -
  E8E00_RS17105 gshB 402496..403440 (-) 945 WP_096629925.1 glutathione synthase -
  E8E00_RS17110 rsmE 403462..404193 (-) 732 WP_069591180.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  E8E00_RS01815 (E8E00_01815) - 404308..404811 (-) 504 WP_096629922.1 SprT family zinc-dependent metalloprotease -
  E8E00_RS01820 (E8E00_01820) metK 404952..406106 (-) 1155 WP_069591183.1 methionine adenosyltransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41051.21 Da        Isoelectric Point: 6.9093

>NTDB_id=359429 E8E00_RS01795 WP_096629928.1 400298..401416(+) (pilU) [Salinivibrio sp. YCSC6]
MKLSSVLVAMQEHTASDAYISVDAPVMLRVDGRLKPLSSPLSLAAINVMLNSAMNEEQKQQFITTREANFAVKRSVGRFR
ISAYWQRDMPSMVIRRIETEIPRLSSLGTPVVLEELAMQKRGLVLVVGATGSGKSTTMAAMMGFRNHYGQGHILTVEDPI
EFVHEHQQCIVSQREIGIDTQDYETALKNALRQAPDMVVIGEIRTPETMNFALQFAETGHLCLATLHANNAYQALERALH
LMPKAQRETFLFDLSMNLKGVIAQQLVPGVNGVGRHGAFELLINNAHVAELIRNGELHALKKAMLSGEHFGMQTFDQSLY
ALYVQGSISEDEALRHADSANELKLMIKTGAKHSAPGSALDHVRIERAGSLD

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=359429 E8E00_RS01795 WP_096629928.1 400298..401416(+) (pilU) [Salinivibrio sp. YCSC6]
ATGAAACTTAGTAGTGTGCTGGTGGCGATGCAAGAGCACACCGCCTCGGATGCGTATATTTCTGTGGATGCGCCTGTGAT
GTTACGGGTGGATGGACGCTTAAAACCTCTGTCTTCGCCGTTGTCGTTGGCGGCAATCAACGTCATGTTGAACAGTGCGA
TGAACGAGGAACAAAAGCAGCAATTTATTACCACGCGGGAAGCGAACTTTGCGGTAAAACGCAGTGTTGGGCGTTTTCGG
ATCAGTGCTTATTGGCAGCGTGATATGCCCAGTATGGTGATCCGCCGCATAGAAACCGAGATTCCGCGTTTGTCATCGTT
AGGCACCCCAGTGGTGCTCGAAGAGCTTGCGATGCAAAAGCGAGGCTTGGTCTTAGTGGTTGGGGCGACGGGGTCGGGTA
AGTCGACAACCATGGCAGCGATGATGGGGTTTCGTAATCACTATGGGCAAGGCCATATCCTAACGGTTGAAGATCCGATT
GAATTTGTTCATGAGCATCAGCAATGCATTGTCAGCCAACGCGAAATTGGTATCGATACCCAGGATTATGAAACAGCGCT
AAAAAATGCGCTCAGGCAAGCGCCAGATATGGTGGTAATTGGTGAAATACGAACGCCAGAAACCATGAACTTCGCGCTTC
AGTTTGCGGAAACGGGCCATCTTTGCTTGGCCACCTTACATGCGAATAACGCCTATCAAGCACTGGAGCGGGCATTACAC
TTGATGCCTAAAGCACAGCGAGAGACCTTTTTGTTTGATTTGTCGATGAACTTAAAAGGTGTTATCGCTCAACAATTGGT
ACCGGGTGTGAATGGGGTTGGGCGGCATGGTGCCTTTGAATTATTGATCAATAACGCGCATGTGGCAGAGCTTATCCGTA
ATGGTGAACTACACGCGTTGAAAAAAGCCATGTTGAGCGGGGAGCATTTTGGTATGCAGACCTTTGATCAATCGCTGTAT
GCGCTTTATGTACAAGGGAGTATCAGCGAGGATGAGGCGCTGCGACATGCCGACTCGGCCAATGAATTAAAGTTGATGAT
CAAAACCGGTGCTAAGCACAGTGCGCCGGGCAGCGCACTGGATCATGTACGGATTGAAAGAGCGGGCTCGCTGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A4GJD3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.929

98.387

0.599
  pilU Pseudomonas stutzeri DSM 10701

53.107

95.161

0.505
  pilU Acinetobacter baylyi ADP1

49.304

96.505

0.476

Multiple sequence alignment