Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E5790_RS04850 Genome accession   NZ_CP039255
Coordinates   1006598..1007635 (-) Length   345 a.a.
NCBI ID   WP_004147099.1    Uniprot ID   A0AAP7J2J0
Organism   Stenotrophomonas sp. PAMC25021     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1001598..1012635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5790_RS04825 (E5790_04820) - 1001963..1002670 (-) 708 WP_014036228.1 YitT family protein -
  E5790_RS04830 (E5790_04825) - 1002781..1002993 (-) 213 WP_014646220.1 DUF4287 domain-containing protein -
  E5790_RS04835 (E5790_04830) - 1003292..1004188 (+) 897 WP_111205533.1 LysR family transcriptional regulator -
  E5790_RS04840 (E5790_04835) - 1004251..1005234 (-) 984 WP_049405029.1 aldo/keto reductase -
  E5790_RS04845 (E5790_04840) pilU 1005356..1006450 (-) 1095 WP_043033789.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5790_RS04850 (E5790_04845) pilT 1006598..1007635 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  E5790_RS04855 (E5790_04850) - 1007723..1008400 (+) 678 WP_111205532.1 YggS family pyridoxal phosphate-dependent enzyme -
  E5790_RS04860 (E5790_04855) proC 1008419..1009240 (+) 822 WP_014646216.1 pyrroline-5-carboxylate reductase -
  E5790_RS04865 (E5790_04860) - 1009300..1010175 (-) 876 WP_014646215.1 LysR family transcriptional regulator -
  E5790_RS04870 (E5790_04865) - 1010274..1010876 (+) 603 WP_014646214.1 short chain dehydrogenase -
  E5790_RS04875 (E5790_04870) soxR 1010949..1011383 (-) 435 WP_021202603.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38658.41 Da        Isoelectric Point: 6.6654

>NTDB_id=357665 E5790_RS04850 WP_004147099.1 1006598..1007635(-) (pilT) [Stenotrophomonas sp. PAMC25021]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=357665 E5790_RS04850 WP_004147099.1 1006598..1007635(-) (pilT) [Stenotrophomonas sp. PAMC25021]
ATGGATATCGCCGAACTGCTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTTCGCCGGATCAACATCCCGGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTGGACTTCTCCTTCGAGATCCCGTCGCTGGCGCGC
TTCCGTGTGAACGCGTTCAACCAGAACCGTGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAGGTGCTCACCCTCGA
GGACCTGGCCTGCCCGCCGCTGTTCCGCGAGGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGTCCCACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGATACGCATGGCTTCAACGA
AGCGCTGCGCTCGGCACTGCGTGAGGACCCGGACATCATCCTGGTGGGCGAACTTCGCGACCTGGAAACCATCCGCCTGG
CACTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGAACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGTTCGATGCTGTCCGAGTCGCTGCGTGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTTGGCGGCGGTCGTACCGCAGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGAGTACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391

Multiple sequence alignment