Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   E5790_RS04845 Genome accession   NZ_CP039255
Coordinates   1005356..1006450 (-) Length   364 a.a.
NCBI ID   WP_043033789.1    Uniprot ID   A0A0X3QW38
Organism   Stenotrophomonas sp. PAMC25021     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1000356..1011450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5790_RS04820 (E5790_04815) - 1001357..1001902 (+) 546 WP_111205534.1 DNA-3-methyladenine glycosylase I -
  E5790_RS04825 (E5790_04820) - 1001963..1002670 (-) 708 WP_014036228.1 YitT family protein -
  E5790_RS04830 (E5790_04825) - 1002781..1002993 (-) 213 WP_014646220.1 DUF4287 domain-containing protein -
  E5790_RS04835 (E5790_04830) - 1003292..1004188 (+) 897 WP_111205533.1 LysR family transcriptional regulator -
  E5790_RS04840 (E5790_04835) - 1004251..1005234 (-) 984 WP_049405029.1 aldo/keto reductase -
  E5790_RS04845 (E5790_04840) pilU 1005356..1006450 (-) 1095 WP_043033789.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5790_RS04850 (E5790_04845) pilT 1006598..1007635 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  E5790_RS04855 (E5790_04850) - 1007723..1008400 (+) 678 WP_111205532.1 YggS family pyridoxal phosphate-dependent enzyme -
  E5790_RS04860 (E5790_04855) proC 1008419..1009240 (+) 822 WP_014646216.1 pyrroline-5-carboxylate reductase -
  E5790_RS04865 (E5790_04860) - 1009300..1010175 (-) 876 WP_014646215.1 LysR family transcriptional regulator -
  E5790_RS04870 (E5790_04865) - 1010274..1010876 (+) 603 WP_014646214.1 short chain dehydrogenase -
  E5790_RS04875 (E5790_04870) soxR 1010949..1011383 (-) 435 WP_021202603.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40569.61 Da        Isoelectric Point: 6.7680

>NTDB_id=357664 E5790_RS04845 WP_043033789.1 1005356..1006450(-) (pilU) [Stenotrophomonas sp. PAMC25021]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=357664 E5790_RS04845 WP_043033789.1 1005356..1006450(-) (pilU) [Stenotrophomonas sp. PAMC25021]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACGGCCGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCC
GATCACCCAGACCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAAT
TCGAGAAGACGCACGAATGCAACTTCGCCATCGGCCTGTCCGGCGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGC
AACCAGGTCGGCATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACCGTGGAAGAACTGAGCCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGCGCCACCGGTACCGGTAAATCGACCTCGCTGGCAGCGA
TGATCGGTTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAG
GGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGC
GCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAAGGCATGGATCACGCCATCGCGTTCGCCGAAACCGGCCACC
TGGTGCTGTGCACCCTGCATGCGAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGC
AACCAGCTGCTGATGGACCTGTCGCTGAATCTCAAGGGCGTGGTCGCGCAACAGCTGGTGCCATCGCCCGATGGCCGCTC
GCGCAAGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGACTCGGTCCAGCTTGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAG
ATCAGCTACGAGGACGCGCTGCGCTACGCCGATTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGA
CGCACGTACCCTGTCGCAGGGGCTGGACGGCGTGGAGATCTCGGAGATCCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X3QW38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.715

98.352

0.676
  pilU Acinetobacter baylyi ADP1

64.08

95.604

0.613
  pilU Vibrio cholerae strain A1552

54.467

95.33

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

94.78

0.407
  pilT Acinetobacter baumannii strain A118

40.303

90.659

0.365
  pilT Acinetobacter nosocomialis M2

40.303

90.659

0.365
  pilT Acinetobacter baumannii D1279779

40.303

90.659

0.365
  pilT Pseudomonas aeruginosa PAK

40.615

89.286

0.363

Multiple sequence alignment