Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E5678_RS14850 Genome accession   NZ_CP039252
Coordinates   3261880..3262923 (+) Length   347 a.a.
NCBI ID   WP_136179244.1    Uniprot ID   A0A4P7RCL6
Organism   Hydrogenophaga sp. PAMC20947     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3256880..3267923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5678_RS14830 (E5678_14830) - 3257001..3259883 (-) 2883 Protein_2948 L-glutamate gamma-semialdehyde dehydrogenase -
  E5678_RS14835 (E5678_14835) - 3259988..3260476 (+) 489 WP_210731922.1 Lrp/AsnC ligand binding domain-containing protein -
  E5678_RS14840 (E5678_14840) - 3260532..3261095 (+) 564 WP_247596792.1 DUF3617 domain-containing protein -
  E5678_RS14845 (E5678_14845) - 3261134..3261841 (-) 708 WP_136179243.1 YggS family pyridoxal phosphate-dependent enzyme -
  E5678_RS14850 (E5678_14850) pilT 3261880..3262923 (+) 1044 WP_136179244.1 type IV pilus twitching motility protein PilT Machinery gene
  E5678_RS14855 (E5678_14855) pilU 3263068..3264204 (+) 1137 WP_136179245.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5678_RS14860 (E5678_14860) - 3264668..3265570 (+) 903 WP_136179246.1 NAD(P)-dependent oxidoreductase -
  E5678_RS14865 (E5678_14865) - 3265648..3266268 (-) 621 WP_136179247.1 BON domain-containing protein -
  E5678_RS14870 (E5678_14870) - 3266265..3266885 (-) 621 WP_136179248.1 SIS domain-containing protein -
  E5678_RS14875 (E5678_14875) - 3266913..3267407 (-) 495 WP_136179249.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38195.84 Da        Isoelectric Point: 6.9312

>NTDB_id=357635 E5678_RS14850 WP_136179244.1 3261880..3262923(+) (pilT) [Hydrogenophaga sp. PAMC20947]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEPLDHKGVHSMVYDIMNDSQRKQYEEFLECDFSFEIEGLSR
FRVNAFNHNRGAGAVFRTIPSKILSLEQLNAPKIFGDLALRPRGMVLVTGPTGSGKSTTLAAMVNHLNETEYGHILTVED
PVEFVHESKKCLINQREVGPHTLSFANALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKDMVRAMLSESLVAVISQTLCKVKDGSGRVAAHEIMIGTSAIRNLIREAKVAQMYSAIQTGNSVGMQTLDQN
LSELVRRNLISPAEARSKAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=357635 E5678_RS14850 WP_136179244.1 3261880..3262923(+) (pilT) [Hydrogenophaga sp. PAMC20947]
ATGGACATCACGCAACTGCTCGCCTTCAGCGTCAAGAACAAAGCATCGGATTTGCACCTCTCGGCAGGCCTGCCGCCCAT
GATACGGGTGCACGGAGACGTGCGCCGGATCAACGTCGAACCGCTGGACCACAAAGGTGTCCACAGCATGGTGTACGACA
TCATGAACGACAGTCAGCGCAAGCAATACGAAGAGTTCCTGGAGTGCGATTTCTCGTTTGAAATCGAAGGTCTGTCGCGT
TTTCGCGTCAATGCGTTCAACCACAACCGCGGCGCCGGCGCGGTGTTTCGAACCATTCCGAGCAAAATCCTGTCACTGGA
GCAGCTCAACGCCCCCAAGATTTTCGGCGACCTCGCGCTGCGTCCACGCGGCATGGTGCTGGTCACGGGCCCGACCGGCT
CGGGCAAATCGACCACGCTGGCCGCCATGGTCAACCACCTGAACGAGACCGAGTACGGCCACATTCTGACCGTGGAAGAC
CCGGTCGAATTCGTGCACGAGTCCAAGAAGTGCCTGATCAACCAACGCGAAGTCGGTCCACACACCCTGTCCTTTGCCAA
CGCCCTGCGCTCGGCCCTGCGGGAAGATCCCGACGCCATTCTGGTGGGTGAGATGCGCGATCTGGAAACCATTCGACTGG
CCATGACGGCCGCGGAAACCGGTCACCTCGTGTTCGGCACGCTGCACACCTCCAGCGCTGCCAAAACCATTGACCGCATC
ATTGACGTGTTCCCGGCAGAAGAAAAAGACATGGTGCGCGCCATGTTGTCGGAATCGCTGGTAGCGGTGATCTCACAAAC
CCTGTGCAAGGTGAAAGATGGCAGCGGCCGGGTCGCAGCGCACGAAATCATGATCGGCACCAGCGCCATCCGAAACCTCA
TCCGTGAGGCCAAGGTCGCGCAGATGTACTCGGCTATCCAGACCGGCAACAGTGTGGGCATGCAGACACTGGACCAGAAT
CTCTCGGAGCTCGTCAGACGCAACCTGATCAGCCCGGCCGAGGCCCGCAGCAAGGCCAAGATTCCGGACAACTTCCCTGG
GTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7RCL6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

72.566

97.695

0.709
  pilT Legionella pneumophila strain ERS1305867

72.566

97.695

0.709
  pilT Neisseria meningitidis 8013

69.412

97.983

0.68
  pilT Neisseria gonorrhoeae MS11

69.118

97.983

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.569

98.271

0.654
  pilT Vibrio cholerae strain A1552

66.569

98.271

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Vibrio cholerae strain A1552

41.194

96.542

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395

Multiple sequence alignment