Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   E5678_RS14855 Genome accession   NZ_CP039252
Coordinates   3263068..3264204 (+) Length   378 a.a.
NCBI ID   WP_136179245.1    Uniprot ID   A0A4P7RAD0
Organism   Hydrogenophaga sp. PAMC20947     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3258068..3269204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5678_RS14835 (E5678_14835) - 3259988..3260476 (+) 489 WP_210731922.1 Lrp/AsnC ligand binding domain-containing protein -
  E5678_RS14840 (E5678_14840) - 3260532..3261095 (+) 564 WP_247596792.1 DUF3617 domain-containing protein -
  E5678_RS14845 (E5678_14845) - 3261134..3261841 (-) 708 WP_136179243.1 YggS family pyridoxal phosphate-dependent enzyme -
  E5678_RS14850 (E5678_14850) pilT 3261880..3262923 (+) 1044 WP_136179244.1 type IV pilus twitching motility protein PilT Machinery gene
  E5678_RS14855 (E5678_14855) pilU 3263068..3264204 (+) 1137 WP_136179245.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5678_RS14860 (E5678_14860) - 3264668..3265570 (+) 903 WP_136179246.1 NAD(P)-dependent oxidoreductase -
  E5678_RS14865 (E5678_14865) - 3265648..3266268 (-) 621 WP_136179247.1 BON domain-containing protein -
  E5678_RS14870 (E5678_14870) - 3266265..3266885 (-) 621 WP_136179248.1 SIS domain-containing protein -
  E5678_RS14875 (E5678_14875) - 3266913..3267407 (-) 495 WP_136179249.1 YraN family protein -
  E5678_RS14880 (E5678_14880) rsmI 3267407..3268360 (+) 954 WP_136179250.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  E5678_RS14885 (E5678_14885) - 3268368..3268688 (-) 321 WP_136179251.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42014.54 Da        Isoelectric Point: 7.2753

>NTDB_id=357636 E5678_RS14855 WP_136179245.1 3263068..3264204(+) (pilU) [Hydrogenophaga sp. PAMC20947]
MERDQASKFINDLLKLMVSRGGSDLFLTAEFPPAIKVDGKVVKVSPQPLNAVHTLSLARAIMNDKQAAEFERTKECNFAI
SPPTVGRFRVSAFIQQGKVGMVLRTIPATLPTIDKLGLPQVLKDVSLSKRGLCILVGATGSGKSTSLAAMVDWRNEHTFG
HIITIEDPVEFVHPHKNCVVTQREVGLDTDNWEAALHNTLRQAPDVILMGEIRTRETMEHAIQFSETGHLCLATLHANSA
NQALDRIINFFPEERRPQLLMDLSLNLRALVSQRLVPRQDNKGRFAAVEIMLNSPLISDLIFKGEVAEIKEIMKKSTQMG
MQTFDQALFHAFEANLITLEDALRNADSLNDLRLQIKLNSQRAKTQDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=357636 E5678_RS14855 WP_136179245.1 3263068..3264204(+) (pilU) [Hydrogenophaga sp. PAMC20947]
ATGGAACGCGATCAGGCATCAAAATTCATCAATGATCTGCTCAAGCTGATGGTCAGCAGGGGTGGCAGTGATCTGTTCCT
GACCGCGGAATTCCCGCCCGCCATCAAGGTCGACGGCAAGGTGGTGAAGGTGTCTCCCCAGCCCCTCAACGCGGTGCACA
CCCTGTCGCTGGCGCGCGCCATCATGAACGACAAGCAGGCGGCGGAATTCGAGCGCACCAAGGAGTGCAACTTCGCCATT
TCGCCACCCACCGTGGGTCGCTTCCGGGTCAGCGCCTTCATCCAGCAAGGCAAGGTCGGCATGGTGTTGCGGACCATTCC
CGCCACGCTGCCCACCATCGACAAACTGGGGCTGCCCCAGGTATTGAAAGACGTGTCGCTGAGCAAGCGCGGTCTGTGCA
TTCTGGTGGGCGCCACAGGCTCAGGCAAGTCCACGTCGCTGGCGGCCATGGTCGACTGGCGCAACGAACACACCTTCGGC
CATATCATCACCATCGAAGACCCGGTCGAATTTGTGCATCCCCACAAAAACTGCGTGGTGACCCAGCGCGAAGTGGGACT
GGACACCGACAACTGGGAAGCGGCTCTGCACAACACGCTGCGCCAGGCACCCGACGTGATCTTGATGGGCGAGATCCGCA
CCCGGGAAACCATGGAACATGCGATCCAGTTCTCGGAAACCGGTCACCTCTGCCTGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCCCGCAACTGCTGATGGACCTGTCGCTCAACTT
GCGCGCGCTGGTTTCCCAGCGGCTGGTGCCGCGCCAGGACAACAAAGGGCGTTTCGCAGCGGTGGAGATCATGTTGAACT
CTCCCCTGATTTCCGACCTGATCTTCAAAGGCGAAGTGGCCGAGATCAAGGAGATCATGAAGAAGAGCACCCAGATGGGC
ATGCAGACCTTCGACCAGGCCTTGTTCCACGCGTTTGAAGCCAATCTCATCACGCTGGAAGACGCTCTGCGCAACGCCGA
TTCGCTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGTGCCAAAACACAGGATCTGGCGGCGGGCACCGAGC
ACCTGACTATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7RAD0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.929

96.825

0.59
  pilU Acinetobacter baylyi ADP1

55.028

94.709

0.521
  pilU Vibrio cholerae strain A1552

52.586

92.063

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Pseudomonas aeruginosa PAK

43.62

89.153

0.389
  pilT Acinetobacter baumannii strain A118

44.201

84.392

0.373
  pilT Acinetobacter nosocomialis M2

44.201

84.392

0.373
  pilT Acinetobacter baumannii D1279779

44.201

84.392

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.84

89.153

0.373
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362
  pilT Legionella pneumophila strain Lp02

41.641

87.037

0.362
  pilT Legionella pneumophila strain ERS1305867

41.641

87.037

0.362

Multiple sequence alignment