Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HNEAP_RS09155 Genome accession   NC_013422
Coordinates   1975671..1976720 (+) Length   349 a.a.
NCBI ID   WP_012824696.1    Uniprot ID   -
Organism   Halothiobacillus neapolitanus c2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1970671..1981720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNEAP_RS09130 (Hneap_1836) metW 1971141..1971743 (-) 603 WP_012824691.1 methionine biosynthesis protein MetW -
  HNEAP_RS09135 (Hneap_1837) metX 1971740..1972951 (-) 1212 WP_012824692.1 homoserine O-succinyltransferase MetX -
  HNEAP_RS09140 (Hneap_1838) - 1973004..1973555 (-) 552 WP_012824693.1 YggT family protein -
  HNEAP_RS09145 (Hneap_1839) proC 1973552..1974400 (-) 849 WP_012824694.1 pyrroline-5-carboxylate reductase -
  HNEAP_RS09150 (Hneap_1840) - 1974685..1975356 (-) 672 WP_012824695.1 YggS family pyridoxal phosphate-dependent enzyme -
  HNEAP_RS09155 (Hneap_1841) pilT 1975671..1976720 (+) 1050 WP_012824696.1 type IV pilus twitching motility protein PilT Machinery gene
  HNEAP_RS09160 (Hneap_1842) pilU 1976774..1977919 (+) 1146 WP_012824697.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HNEAP_RS09165 (Hneap_1843) - 1978022..1979185 (+) 1164 WP_012824698.1 PilT/PilU family type 4a pilus ATPase -
  HNEAP_RS09170 (Hneap_1844) - 1980058..1980408 (+) 351 WP_012824699.1 c-type cytochrome -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38783.35 Da        Isoelectric Point: 7.0292

>NTDB_id=35531 HNEAP_RS09155 WP_012824696.1 1975671..1976720(+) (pilT) [Halothiobacillus neapolitanus c2]
MAEQRGIDSLLTFSVKNGASDLHLSAGEQPRVRIDGDVRKVNVPVMEHKDVHAMVYDIMNDKQRKEYEDNLEVDFSFELP
GVARFRVNAFNQNRGAAAVFRTIPSEILSLEQLATPSVFKDLCLFPRGIVLVTGPTGSGKSTTLAAMIDYINDNRNDHIL
TIEDPIEFVHNSKKCLINQREVHRDTHSFNAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKT
IDRVVDVFPAAEKDMVRSMLSESLRAVISQTLLKKIGGGRIAAHEIMLGSPAIRNLIRENKIAQMYSAIQTGQTQGMQTL
DQNLQQLVKRGLVAKDEARARAQNKTDFM

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=35531 HNEAP_RS09155 WP_012824696.1 1975671..1976720(+) (pilT) [Halothiobacillus neapolitanus c2]
ATGGCAGAACAACGCGGTATTGATTCCCTTCTTACGTTTTCAGTAAAAAACGGCGCCTCAGATCTTCATCTGTCTGCCGG
TGAGCAGCCTCGCGTGCGGATCGACGGCGATGTGCGCAAAGTCAACGTGCCCGTTATGGAGCACAAAGACGTGCACGCCA
TGGTGTACGACATCATGAACGACAAGCAACGCAAGGAATACGAAGATAATCTTGAGGTGGATTTTTCGTTCGAACTTCCC
GGCGTGGCTCGCTTCCGGGTTAACGCATTCAACCAGAACCGCGGAGCGGCTGCGGTATTTCGAACCATTCCCAGTGAAAT
CCTATCTCTTGAACAATTGGCCACGCCAAGTGTGTTCAAGGATTTGTGCCTGTTTCCGCGCGGCATCGTGCTGGTCACCG
GCCCGACGGGTTCCGGTAAATCAACGACTCTGGCCGCGATGATCGATTACATCAACGATAATCGTAACGACCACATTCTG
ACGATCGAAGACCCGATCGAATTCGTTCACAACAGTAAAAAGTGCCTGATCAACCAACGTGAAGTGCATCGCGATACCCA
CAGTTTCAATGCGGCACTTCGCTCGGCCTTACGCGAAGATCCCGATGTGATTCTGGTCGGCGAGTTGCGCGATCTGGAAA
CCATCCGTCTGGCGCTCACCGCAGCGGAAACCGGCCACCTGGTGTTTGCGACCTTGCACACAAGTTCTGCCGCCAAAACC
ATCGACCGCGTGGTGGATGTGTTCCCCGCTGCGGAGAAAGATATGGTTCGTTCGATGCTGTCAGAATCACTACGCGCTGT
AATCTCCCAAACACTGCTCAAGAAAATCGGTGGTGGGCGGATTGCTGCGCACGAGATCATGCTGGGCAGCCCGGCGATTC
GTAACCTGATCCGTGAAAACAAAATCGCCCAAATGTACTCGGCCATTCAAACCGGCCAAACACAGGGCATGCAGACACTC
GATCAGAACTTGCAGCAGCTCGTCAAACGCGGGCTGGTCGCTAAAGACGAAGCCCGTGCCAGAGCACAAAACAAAACGGA
CTTCATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.968

98.281

0.756
  pilT Acinetobacter baumannii strain A118

76.968

98.281

0.756
  pilT Acinetobacter baumannii D1279779

76.968

98.281

0.756
  pilT Pseudomonas aeruginosa PAK

74.269

97.994

0.728
  pilT Pseudomonas stutzeri DSM 10701

73.392

97.994

0.719
  pilT Acinetobacter baylyi ADP1

73.392

97.994

0.719
  pilT Legionella pneumophila strain Lp02

72.222

97.994

0.708
  pilT Legionella pneumophila strain ERS1305867

72.222

97.994

0.708
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.217

96.562

0.688
  pilT Vibrio cholerae strain A1552

71.217

96.562

0.688
  pilT Neisseria meningitidis 8013

69.912

97.135

0.679
  pilT Neisseria gonorrhoeae MS11

69.617

97.135

0.676
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.422

99.14

0.49
  pilU Pseudomonas stutzeri DSM 10701

40.816

98.281

0.401
  pilU Vibrio cholerae strain A1552

40.816

98.281

0.401
  pilU Acinetobacter baylyi ADP1

40.312

91.691

0.37

Multiple sequence alignment