Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HNEAP_RS09160 Genome accession   NC_013422
Coordinates   1976774..1977919 (+) Length   381 a.a.
NCBI ID   WP_012824697.1    Uniprot ID   D0L1U1
Organism   Halothiobacillus neapolitanus c2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1971774..1982919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNEAP_RS09140 (Hneap_1838) - 1973004..1973555 (-) 552 WP_012824693.1 YggT family protein -
  HNEAP_RS09145 (Hneap_1839) proC 1973552..1974400 (-) 849 WP_012824694.1 pyrroline-5-carboxylate reductase -
  HNEAP_RS09150 (Hneap_1840) - 1974685..1975356 (-) 672 WP_012824695.1 YggS family pyridoxal phosphate-dependent enzyme -
  HNEAP_RS09155 (Hneap_1841) pilT 1975671..1976720 (+) 1050 WP_012824696.1 type IV pilus twitching motility protein PilT Machinery gene
  HNEAP_RS09160 (Hneap_1842) pilU 1976774..1977919 (+) 1146 WP_012824697.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HNEAP_RS09165 (Hneap_1843) - 1978022..1979185 (+) 1164 WP_012824698.1 PilT/PilU family type 4a pilus ATPase -
  HNEAP_RS09170 (Hneap_1844) - 1980058..1980408 (+) 351 WP_012824699.1 c-type cytochrome -
  HNEAP_RS09175 (Hneap_1845) - 1980417..1981775 (+) 1359 WP_012824700.1 FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42700.57 Da        Isoelectric Point: 5.7778

>NTDB_id=35532 HNEAP_RS09160 WP_012824697.1 1976774..1977919(+) (pilU) [Halothiobacillus neapolitanus c2]
MDRQQAANYVQGLLRKAVELDASDLFVTVGAPPSAKINGEITHLTETSLSTNHTQMLVRSIMTDKQIASFEETREANFAI
SLPGVARFRVNAFMQRGSVGMVLRMIRSDVPKFSELLLPEILETISMTKRGLVIFVGATSSGKSTSLAAMLDYRNENSAG
HIVTIEDPIEYVHRHKGCLVTQREVGVDTDTFESALKNTLRQAPDVILIGEIRDRETMDHAIAFAETGHLCLATLHANNT
NQALDRIINFFPEERRHQLLMDLSLNLKSVVSQRLLRKEDGNGRVPAVEVLINTPLIGDLIFKGDVHEIKEVMKRSREHG
MQTFDQALLDLYDEGLVSFQEALRNADSQNDLRLNIKLHSRRGLPKDFEDGNDQLRVESDD

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=35532 HNEAP_RS09160 WP_012824697.1 1976774..1977919(+) (pilU) [Halothiobacillus neapolitanus c2]
ATGGATCGCCAGCAAGCAGCTAACTACGTTCAGGGCTTATTACGCAAAGCAGTGGAGCTTGATGCCTCCGACCTATTCGT
AACGGTAGGCGCCCCACCGAGTGCAAAAATCAACGGGGAAATCACCCACCTGACCGAAACAAGCTTGTCGACCAACCATA
CCCAAATGTTGGTTCGGTCGATCATGACCGACAAGCAGATCGCCTCGTTTGAAGAAACGCGCGAAGCGAATTTCGCGATT
TCTCTGCCCGGTGTCGCCCGCTTCCGTGTCAATGCGTTCATGCAGCGCGGCAGTGTGGGGATGGTTCTGCGGATGATTCG
ATCCGACGTGCCCAAATTCTCCGAATTGTTATTACCAGAAATCCTCGAAACCATTTCCATGACCAAACGTGGCTTGGTGA
TTTTCGTTGGCGCGACCAGCTCGGGGAAGTCGACCTCGCTTGCTGCCATGCTCGATTACCGCAACGAAAACTCGGCAGGC
CACATTGTTACCATCGAAGACCCGATTGAATACGTGCACCGACACAAAGGTTGCCTTGTGACCCAGCGTGAAGTGGGCGT
GGACACCGATACGTTCGAATCGGCGTTGAAAAACACCCTTCGACAAGCGCCAGATGTCATCCTGATTGGCGAAATCAGGG
ATCGCGAAACGATGGATCATGCCATTGCCTTCGCCGAAACCGGGCATCTTTGCCTGGCTACGCTGCACGCCAACAACACC
AATCAGGCACTGGACCGCATCATCAACTTCTTTCCGGAAGAACGTCGCCATCAACTGCTGATGGACCTTTCCCTGAACCT
GAAATCGGTTGTTTCCCAACGGCTGCTGCGCAAGGAAGACGGTAACGGCCGCGTTCCAGCGGTCGAAGTGTTGATCAACA
CGCCATTGATCGGCGACCTGATCTTCAAGGGCGACGTGCACGAAATCAAGGAAGTGATGAAACGCTCCCGCGAACACGGC
ATGCAAACTTTCGACCAAGCGTTGCTGGATCTGTACGACGAAGGCCTGGTGTCCTTCCAGGAAGCCCTGCGCAATGCGGA
TTCACAAAACGACCTTCGCCTTAATATCAAACTGCACAGTCGTCGCGGCCTACCGAAGGACTTCGAGGATGGGAACGATC
AATTGCGCGTTGAAAGTGATGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D0L1U1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.931

91.339

0.575
  pilU Acinetobacter baylyi ADP1

58.989

93.438

0.551
  pilU Vibrio cholerae strain A1552

54.768

96.325

0.528
  pilT Acinetobacter baumannii strain A118

44.807

88.451

0.396
  pilT Acinetobacter nosocomialis M2

44.807

88.451

0.396
  pilT Acinetobacter baumannii D1279779

44.807

88.451

0.396
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.611

87.664

0.391
  pilT Pseudomonas aeruginosa PAK

44.311

87.664

0.388
  pilT Acinetobacter baylyi ADP1

43.75

88.189

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.713

87.664

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.159

90.551

0.373
  pilT Vibrio cholerae strain A1552

41.159

90.551

0.373
  pilT Neisseria meningitidis 8013

41.056

89.501

0.367
  pilT Neisseria gonorrhoeae MS11

40.588

89.239

0.362

Multiple sequence alignment