Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E2K93_RS11975 Genome accession   NZ_CP038493
Coordinates   2770237..2771280 (+) Length   347 a.a.
NCBI ID   WP_135439318.1    Uniprot ID   -
Organism   Thalassotalea sp. HSM 43     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2765237..2776280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2K93_RS11940 (E2K93_11940) - 2765424..2765642 (+) 219 WP_135439311.1 hypothetical protein -
  E2K93_RS11945 (E2K93_11945) hemW 2765689..2766834 (-) 1146 WP_135439312.1 radical SAM family heme chaperone HemW -
  E2K93_RS11950 (E2K93_11950) - 2766844..2767434 (-) 591 WP_135439313.1 XTP/dITP diphosphatase -
  E2K93_RS11955 (E2K93_11955) - 2767521..2767964 (-) 444 WP_135439314.1 DUF4426 domain-containing protein -
  E2K93_RS11960 (E2K93_11960) - 2768048..2768605 (-) 558 WP_135439315.1 YggT family protein -
  E2K93_RS11965 (E2K93_11965) proC 2768698..2769513 (-) 816 WP_135439316.1 pyrroline-5-carboxylate reductase -
  E2K93_RS11970 (E2K93_11970) - 2769517..2770197 (-) 681 WP_135439317.1 YggS family pyridoxal phosphate-dependent enzyme -
  E2K93_RS11975 (E2K93_11975) pilT 2770237..2771280 (+) 1044 WP_135439318.1 type IV pilus twitching motility protein PilT Machinery gene
  E2K93_RS11980 (E2K93_11980) pilU 2771290..2772405 (+) 1116 WP_135439319.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E2K93_RS11985 (E2K93_11985) metK 2772716..2773906 (+) 1191 WP_135439320.1 methionine adenosyltransferase -
  E2K93_RS11990 (E2K93_11990) rsmE 2774063..2774791 (+) 729 WP_135439321.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  E2K93_RS11995 (E2K93_11995) gshB 2774821..2775777 (+) 957 WP_135439322.1 glutathione synthase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38700.30 Da        Isoelectric Point: 5.4508

>NTDB_id=354747 E2K93_RS11975 WP_135439318.1 2770237..2771280(+) (pilT) [Thalassotalea sp. HSM 43]
MDITELLAFSVEHNASDLHLSTGMPPAIRVDGDVRKINIPPLEDKDVNALVYDIMTDRQRKEYEENLEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSKILSLDELGCPDIFRQISENPRGLVLVTGPTGSGKSTTLAAMVDYINESKRDHILTIED
PIEFVHDNKQCLINQREVHRDTLSFSNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPGEEKDMVRSMLSESLRAVISQTLIKKVGGGRVAAHEIMVGIPAIRNLIREDKIAQMYSAIQTGMSHGMQTMDQCL
QGLLSRGLITREDAMAKAADKNQFKVY

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=354747 E2K93_RS11975 WP_135439318.1 2770237..2771280(+) (pilT) [Thalassotalea sp. HSM 43]
ATGGATATTACCGAACTTTTAGCGTTTAGTGTTGAACACAACGCATCAGATTTACACCTTTCCACTGGTATGCCACCCGC
TATTCGTGTTGATGGCGATGTACGTAAGATTAACATTCCACCGCTAGAAGACAAAGATGTAAATGCTCTAGTGTATGACA
TCATGACCGATCGTCAGCGCAAAGAATACGAAGAAAATCTTGAAGTGGATTTCTCCTTTGAAGTGCCAAACCTAGCTCGT
TTCAGGGTAAATGCGTTCAATCAGAACCGAGGCCCTGCTGCTGTATTTCGTACCATCCCAAGTAAAATTTTATCATTGGA
TGAGTTGGGTTGCCCAGATATTTTCCGCCAAATCTCTGAAAACCCTCGTGGTTTAGTATTGGTCACCGGTCCAACGGGTT
CGGGTAAATCAACAACCTTGGCAGCGATGGTAGATTACATCAACGAATCTAAGCGCGATCACATTCTAACCATCGAAGAT
CCAATCGAATTCGTGCACGACAACAAGCAATGTCTGATTAACCAACGTGAAGTGCACCGTGATACGTTAAGCTTTAGCAA
CGCGTTGCGTAGTGCGCTACGTGAAGATCCCGATGTTATTCTGGTTGGTGAGATGCGTGACCTTGAAACCATTCGTCTTG
CGATGACCGCGGCAGAAACCGGTCACTTAGTATTTGGTACCTTGCATACCACCTCGGCACCAAAAACCATTGACCGTATT
GTTGATGTATTCCCTGGTGAAGAAAAAGACATGGTTCGCTCTATGTTGTCTGAATCATTGCGTGCGGTAATTTCGCAAAC
ACTGATTAAGAAAGTAGGTGGTGGCCGAGTTGCTGCACACGAAATCATGGTCGGTATCCCGGCGATTCGTAACCTGATTC
GTGAAGATAAGATTGCGCAAATGTACTCGGCAATTCAAACCGGTATGTCACATGGTATGCAAACCATGGATCAATGTTTA
CAAGGCTTATTAAGTCGTGGCCTGATCACCCGTGAAGACGCTATGGCAAAAGCAGCTGATAAGAATCAGTTTAAAGTTTA
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.616

99.135

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.744

99.135

0.761
  pilT Acinetobacter nosocomialis M2

76.744

99.135

0.761
  pilT Acinetobacter baumannii D1279779

76.453

99.135

0.758
  pilT Acinetobacter baumannii strain A118

76.453

99.135

0.758
  pilT Acinetobacter baylyi ADP1

75.581

99.135

0.749
  pilT Legionella pneumophila strain Lp02

72.674

99.135

0.72
  pilT Legionella pneumophila strain ERS1305867

72.674

99.135

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.92

97.118

0.689
  pilT Vibrio cholerae strain A1552

70.92

97.118

0.689
  pilT Neisseria meningitidis 8013

69.208

98.271

0.68
  pilT Neisseria gonorrhoeae MS11

68.915

98.271

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.623

99.424

0.533
  pilU Vibrio cholerae strain A1552

40.922

100

0.409
  pilU Pseudomonas stutzeri DSM 10701

39.481

100

0.395
  pilU Acinetobacter baylyi ADP1

39.08

100

0.392

Multiple sequence alignment