Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   E2K93_RS11980 Genome accession   NZ_CP038493
Coordinates   2771290..2772405 (+) Length   371 a.a.
NCBI ID   WP_135439319.1    Uniprot ID   A0A4P7JN88
Organism   Thalassotalea sp. HSM 43     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2766290..2777405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2K93_RS11950 (E2K93_11950) - 2766844..2767434 (-) 591 WP_135439313.1 XTP/dITP diphosphatase -
  E2K93_RS11955 (E2K93_11955) - 2767521..2767964 (-) 444 WP_135439314.1 DUF4426 domain-containing protein -
  E2K93_RS11960 (E2K93_11960) - 2768048..2768605 (-) 558 WP_135439315.1 YggT family protein -
  E2K93_RS11965 (E2K93_11965) proC 2768698..2769513 (-) 816 WP_135439316.1 pyrroline-5-carboxylate reductase -
  E2K93_RS11970 (E2K93_11970) - 2769517..2770197 (-) 681 WP_135439317.1 YggS family pyridoxal phosphate-dependent enzyme -
  E2K93_RS11975 (E2K93_11975) pilT 2770237..2771280 (+) 1044 WP_135439318.1 type IV pilus twitching motility protein PilT Machinery gene
  E2K93_RS11980 (E2K93_11980) pilU 2771290..2772405 (+) 1116 WP_135439319.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E2K93_RS11985 (E2K93_11985) metK 2772716..2773906 (+) 1191 WP_135439320.1 methionine adenosyltransferase -
  E2K93_RS11990 (E2K93_11990) rsmE 2774063..2774791 (+) 729 WP_135439321.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  E2K93_RS11995 (E2K93_11995) gshB 2774821..2775777 (+) 957 WP_135439322.1 glutathione synthase -
  E2K93_RS12000 (E2K93_12000) - 2775950..2776504 (+) 555 WP_135439323.1 YqgE/AlgH family protein -
  E2K93_RS12005 (E2K93_12005) - 2776720..2777202 (-) 483 WP_135439324.1 FKBP-type peptidyl-prolyl cis-trans isomerase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40965.84 Da        Isoelectric Point: 5.4153

>NTDB_id=354748 E2K93_RS11980 WP_135439319.1 2771290..2772405(+) (pilU) [Thalassotalea sp. HSM 43]
MYLHELLSNMVSQDASDLFITVNLAASAKINGELVAIDEKVLSYDEALDLVLSSMNDKNKAEYEATKECNYAIAVDGIGR
FRISAFWQREMPGMVVRRIVTDIPDVDKLGLPQVLKDLVMSKRGLVLFVGGTGTGKSTSMASLIGYRNRNARGHILTIED
PVEFVHEHARSMITQREVGMDTESFDAALKSSLRQAPDVILIGEIRSQEIMEHALSFAETGHLCIATLHANNANQAIDRI
MHLVPAEQHQKLQFDLALNLRGIVAQQLVPTRDGHGRRAAIEILLNSPYIAELIKKGDVGQIKEVMTKSRELGMQTFDQA
LFDLYESGDISYTDALHHADSPNDLRLMIKLRSNDQTGIGGLKGATLDGVE

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=354748 E2K93_RS11980 WP_135439319.1 2771290..2772405(+) (pilU) [Thalassotalea sp. HSM 43]
ATGTATTTACACGAACTGTTAAGCAATATGGTCAGCCAAGATGCATCGGATTTATTTATAACCGTTAATCTAGCTGCCAG
TGCCAAAATCAATGGCGAGCTAGTCGCCATTGATGAAAAGGTTCTCAGTTATGATGAGGCACTTGATCTTGTCTTAAGCT
CGATGAATGACAAGAACAAAGCTGAATATGAAGCCACCAAAGAATGTAACTACGCTATCGCCGTAGATGGTATTGGCCGT
TTTCGTATTTCAGCGTTTTGGCAACGCGAAATGCCCGGCATGGTTGTTCGTCGCATCGTTACCGATATACCGGATGTCGA
CAAGCTAGGCTTGCCACAGGTATTGAAAGACTTGGTGATGTCAAAACGTGGCTTGGTATTGTTTGTCGGTGGTACCGGTA
CGGGTAAGTCAACGTCAATGGCGTCGTTAATTGGTTATCGTAACCGTAATGCGCGTGGTCACATATTAACCATCGAAGAT
CCGGTGGAATTTGTTCATGAGCATGCCCGTTCAATGATCACTCAACGCGAAGTCGGCATGGATACTGAATCCTTTGATGC
TGCTTTGAAGAGCTCATTGCGACAAGCGCCAGATGTTATTCTGATTGGTGAGATTCGCTCGCAAGAAATCATGGAGCATG
CACTAAGCTTTGCTGAAACCGGCCATTTATGTATTGCTACACTGCATGCTAACAACGCCAACCAAGCAATTGACCGAATT
ATGCACTTGGTACCCGCTGAGCAACATCAAAAATTACAGTTTGATTTAGCCTTGAACTTACGCGGCATTGTCGCCCAACA
GTTGGTACCGACCCGTGACGGTCATGGTCGCCGCGCTGCTATCGAAATTCTATTGAACTCACCTTATATCGCTGAGTTAA
TCAAAAAAGGCGATGTCGGTCAGATCAAAGAGGTAATGACGAAGTCTCGTGAATTGGGCATGCAAACCTTCGATCAGGCG
CTGTTTGATTTGTATGAATCCGGTGATATCAGTTACACCGATGCATTGCATCACGCCGATTCACCAAACGATCTACGCTT
AATGATCAAACTTCGCAGTAACGATCAAACCGGTATTGGTGGCTTAAAAGGCGCAACACTGGACGGCGTTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7JN88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.64

95.148

0.558
  pilU Vibrio cholerae strain A1552

55.978

99.191

0.555
  pilU Acinetobacter baylyi ADP1

58.239

94.879

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.947

96.765

0.396
  pilT Acinetobacter nosocomialis M2

39.181

92.183

0.361
  pilT Acinetobacter baumannii D1279779

39.181

92.183

0.361
  pilT Acinetobacter baumannii strain A118

39.181

92.183

0.361

Multiple sequence alignment