Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EBI04_RS10305 Genome accession   NZ_CP038452
Coordinates   1966729..1967817 (-) Length   362 a.a.
NCBI ID   WP_135257379.1    Uniprot ID   -
Organism   Thermus caldilimi strain YIM 78456     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1961729..1972817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBI04_RS10290 - 1963657..1964285 (-) 629 Protein_2047 histidine phosphatase family protein -
  EBI04_RS10295 purM 1964282..1965283 (-) 1002 WP_135257940.1 phosphoribosylformylglycinamidine cyclo-ligase -
  EBI04_RS10300 gatB 1965289..1966698 (-) 1410 WP_135257378.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  EBI04_RS10305 pilT 1966729..1967817 (-) 1089 WP_135257379.1 type IV pilus twitching motility protein PilT Machinery gene
  EBI04_RS10310 pilF 1967829..1970498 (-) 2670 WP_135257380.1 type IV pilus assembly ATPase PilB Machinery gene
  EBI04_RS10315 - 1970495..1970977 (-) 483 WP_135257381.1 YqeG family HAD IIIA-type phosphatase -
  EBI04_RS10320 pgeF 1971017..1971741 (-) 725 Protein_2053 peptidoglycan editing factor PgeF -
  EBI04_RS10325 - 1971821..1972225 (+) 405 WP_135257382.1 DUF4395 family protein -
  EBI04_RS10330 ribH 1972212..1972685 (-) 474 WP_135257383.1 6,7-dimethyl-8-ribityllumazine synthase -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 40037.30 Da        Isoelectric Point: 7.2368

>NTDB_id=354484 EBI04_RS10305 WP_135257379.1 1966729..1967817(-) (pilT) [Thermus caldilimi strain YIM 78456]
MAKALDIVDLLTLAAERGASDLVITVGLPPMIKVDGEFHPTEYETLSPQDTRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPATIKGFEELGLPKNIAEIALSPRGLVLVTGPTGSGKSTTLASMIDYINERKPVHIV
TIEDPIEFFHKHKKAIVNQREIGSDTHGFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIIDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKSHQLRSVIQTGGQYGMIT
MDACLADLYRRKLITYELGLSRAVDPKEFMRLAGVQEGSKRP

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=354484 EBI04_RS10305 WP_135257379.1 1966729..1967817(-) (pilT) [Thermus caldilimi strain YIM 78456]
ATGGCCAAAGCACTGGATATCGTGGATCTTCTGACCCTGGCGGCGGAGCGGGGGGCCAGCGACCTGGTGATCACCGTGGG
CCTTCCCCCCATGATCAAGGTGGACGGGGAGTTCCACCCCACGGAGTACGAGACCCTCTCCCCCCAGGACACCCGCCGGC
TGATGTACGCCCTCATGGACGAGAAGCAGCAGCGGATCTTTGAGGAGGAAAAGGAGCTGGACTTCTCCTTCAGCCTCCCG
GGAAAAGGGCGCTACCGGGTGAACGTCTTCCTGCAACGGGGAAGCGTGGGAGGGGTCTTAAGGGTGGTGCCCGCCACCAT
CAAGGGCTTTGAGGAGCTGGGCCTGCCCAAGAACATCGCCGAGATCGCCCTAAGCCCCCGGGGCCTGGTCCTGGTCACGG
GGCCCACCGGGTCGGGGAAGAGCACCACCTTAGCCTCCATGATCGACTACATCAACGAGCGCAAGCCGGTGCACATCGTG
ACCATCGAGGATCCCATAGAGTTCTTCCACAAGCACAAGAAGGCCATCGTCAACCAGCGGGAAATCGGCTCCGACACCCA
CGGCTTCCACAAGGCGCTTAGAAGCGTTCTCCGCCAGGCCCCAGACGTGATCCTGGTGGGGGAGATGCGGGACTACGAAA
CCATCGCCGCCGCCATCACCGCCGCGGAGACCGGGCACCTGGTCATGGGCACCCTGCACACCAACTCCGCCCCGGAGACC
ATCGACCGCATCATCGACGTCTTCCCGGAAAATCAGCAAGAGCAGGTGCGGGTGCAGCTTTCCAACAACCTGGTGGCCGT
CCTCACCCAGCAGCTCCTGCCCAAGGCCTTCGGGGGCGGTCGGGTGCTGGCCTACGAGCTCATGATCGCCACCCCTGCGG
TAAGGGCTTTGATCCGGGAGGGCAAGAGCCACCAGCTTAGGAGCGTCATCCAGACGGGCGGCCAGTACGGGATGATCACC
ATGGACGCCTGCCTGGCCGACCTCTACCGGCGCAAGCTCATCACCTACGAGCTGGGCCTCTCCCGGGCGGTGGACCCCAA
GGAGTTCATGCGCCTGGCCGGGGTGCAGGAGGGGAGCAAGCGCCCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

68.272

97.514

0.666
  pilT Pseudomonas stutzeri DSM 10701

54.203

95.304

0.517
  pilT Pseudomonas aeruginosa PAK

54.203

95.304

0.517
  pilT Acinetobacter baumannii strain A118

53.757

95.58

0.514
  pilT Acinetobacter nosocomialis M2

53.757

95.58

0.514
  pilT Acinetobacter baumannii D1279779

53.757

95.58

0.514
  pilT Acinetobacter baylyi ADP1

53.623

95.304

0.511
  pilT Neisseria meningitidis 8013

52.647

93.923

0.494
  pilT Neisseria gonorrhoeae MS11

51.917

93.646

0.486
  pilT Legionella pneumophila strain ERS1305867

50.725

95.304

0.483
  pilT Legionella pneumophila strain Lp02

50.725

95.304

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.121

91.16

0.475
  pilT Vibrio cholerae strain A1552

52.121

91.16

0.475
  pilU Pseudomonas stutzeri DSM 10701

47.23

94.751

0.448
  pilU Acinetobacter baylyi ADP1

40.947

99.171

0.406
  pilU Vibrio cholerae strain A1552

46.129

85.635

0.395

Multiple sequence alignment