Detailed information NTG0354485

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: EBI04_RS10310
• Genome accession: NZ_CP038452
• Coordinates: 1967829..1970498 (-)
• Length: 889 a.a.
• NCBI ID: WP_135257380.1
• Uniprot ID: -
• Organism: Thermus caldilimi strain YIM 78456
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1962829..1975498

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EBI04_RS10290	-	1963657..1964285 (-)	629	Protein_2047	histidine phosphatase family protein	-
EBI04_RS10295	purM	1964282..1965283 (-)	1002	WP_135257940.1	phosphoribosylformylglycinamidine cyclo-ligase	-
EBI04_RS10300	gatB	1965289..1966698 (-)	1410	WP_135257378.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
EBI04_RS10305	pilT	1966729..1967817 (-)	1089	WP_135257379.1	type IV pilus twitching motility protein PilT	Machinery gene
EBI04_RS10310	pilF	1967829..1970498 (-)	2670	WP_135257380.1	type IV pilus assembly ATPase PilB	Machinery gene
EBI04_RS10315	-	1970495..1970977 (-)	483	WP_135257381.1	YqeG family HAD IIIA-type phosphatase	-
EBI04_RS10320	pgeF	1971017..1971741 (-)	725	Protein_2053	peptidoglycan editing factor PgeF	-
EBI04_RS10325	-	1971821..1972225 (+)	405	WP_135257382.1	DUF4395 family protein	-
EBI04_RS10330	ribH	1972212..1972685 (-)	474	WP_135257383.1	6,7-dimethyl-8-ribityllumazine synthase	-
EBI04_RS10335	crcB	1972706..1973083 (+)	378	WP_038071939.1	fluoride efflux transporter CrcB	-
EBI04_RS10340	-	1973088..1973420 (+)	333	WP_135257384.1	DUF190 domain-containing protein	-
EBI04_RS10345	-	1973465..1974079 (+)	615	WP_135257385.1	sulfite oxidase-like oxidoreductase	-
EBI04_RS10350	-	1974076..1974345 (+)	270	WP_135257386.1	hypothetical protein	-
EBI04_RS10355	-	1974321..1975124 (-)	804	WP_135257387.1	histidinol-phosphatase HisJ family protein	-

Sequence

Protein

• Length: 889 a.a.
• Molecular weight: 98187.21 Da
• Isoelectric Point: 5.1272

>NTDB_id=354485 EBI04_RS10310 WP_135257380.1 1967829..1970498(-) (pilF) [Thermus caldilimi strain YIM 78456]
MSVLTIGDKRLGAILLDAGLLTDEELQMALEKHREVGGSLAEVIVDSGLLSERRIAQAIEDHFGIPLVELHTLEIPPKVK
ALLPAEKAKELQAIPFALDEEAGVVRVAFVNPLDTLALEEVEDLTGMVVEPYQATKSAFLYALAKNYPELNLPLPPPPSG
PSREELKVGELLVEKGLLDRNTLEEALVEQEKTGDLLGRILVRKGLPEEVLYRVLAEQKGMEFLPSTQGLSPDPAATNLL
LRSDALRYSAVPVGFRNGEVEVVLADPRHKEAVAELLGRPTRFFLTLPKEWEALFHQAYPEKSRLGEVLVQEGRLSREDL
REALEVQRRLPKAKPLGEILVELGLARPEDVEEALRKQRQGGGRLEDTLVASGKLKPEALAQAVAAQLGYPYINPEENPP
DPGAALLLPEDLARRYGVFPHHVENGKTLVLLMKDPRNILALDDVRLALKRKGLAYEVAPAVATEAAITKLIERFYGKEE
LGEIAKELSKGYQEEEAVTPELDESAAQKFVKQVIREAYLQDASDIHIEPRQFDVLVRLRIDGTLRQYTTLSKGALNSII
SVVKIMGGLNIAERRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKAADIPEIEGLGFAPGVFERFQEVIQKP
YGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIR
DSETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVELFNISAALIGVLSQRLVRRICDHCKVEVRPDPEVLRRLGL
SQEEIQGAKLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARKKGMKTLREDGIYKAFQGITTL
EEVLARTIE

Nucleotide

• Length: 2670 bp

>NTDB_id=354485 EBI04_RS10310 WP_135257380.1 1967829..1970498(-) (pilF) [Thermus caldilimi strain YIM 78456]
ATGAGCGTGCTTACCATCGGGGACAAACGGCTAGGTGCCATCCTATTGGATGCCGGCCTCCTCACCGACGAGGAGCTGCA
GATGGCCCTGGAGAAACACCGGGAGGTGGGGGGTTCCTTGGCCGAGGTCATCGTGGACTCGGGGCTGCTTTCGGAACGGC
GGATCGCCCAGGCCATCGAGGACCACTTCGGCATCCCCCTGGTGGAGCTTCACACCTTGGAGATCCCTCCCAAGGTGAAG
GCCCTCCTGCCGGCGGAGAAGGCCAAGGAGCTCCAGGCCATCCCCTTTGCCCTGGACGAGGAGGCAGGGGTGGTGCGGGT
GGCCTTCGTGAACCCCCTGGACACCTTGGCCCTCGAGGAGGTGGAGGACCTCACCGGGATGGTGGTGGAACCCTACCAGG
CCACCAAAAGCGCCTTCCTCTATGCCCTAGCCAAGAACTACCCCGAACTCAACCTGCCCCTACCCCCTCCCCCCAGCGGC
CCCAGCCGGGAGGAGCTGAAGGTGGGCGAGCTTCTGGTGGAGAAGGGCCTCCTGGACCGGAACACCCTGGAGGAAGCCCT
GGTGGAGCAGGAAAAGACCGGGGATCTCCTGGGCCGCATCCTGGTGCGCAAGGGGCTACCCGAGGAGGTCCTCTACCGGG
TTTTGGCCGAGCAGAAGGGAATGGAGTTTCTCCCCTCCACCCAGGGCCTATCCCCCGACCCCGCCGCCACCAACCTTCTC
CTGCGCTCGGATGCCCTGCGCTATAGCGCCGTGCCCGTGGGGTTCAGAAACGGGGAGGTGGAGGTGGTCCTTGCCGATCC
CCGGCACAAGGAGGCGGTGGCGGAGCTCCTTGGGCGCCCCACCCGTTTCTTCCTCACCCTTCCCAAGGAGTGGGAAGCCC
TTTTCCACCAGGCCTACCCGGAAAAAAGCCGCCTGGGGGAGGTCCTGGTCCAGGAGGGGCGCCTGAGCCGCGAGGACTTA
AGGGAGGCCCTCGAGGTGCAAAGGCGCCTGCCCAAGGCCAAGCCCCTGGGAGAGATCCTGGTGGAGCTGGGCTTGGCCCG
GCCCGAGGACGTGGAGGAGGCCTTGAGGAAGCAACGCCAGGGTGGGGGCCGCCTCGAGGACACCCTGGTGGCCTCGGGCA
AGCTCAAACCCGAGGCCTTAGCCCAGGCGGTGGCCGCCCAGCTGGGCTACCCCTACATCAACCCCGAGGAGAACCCCCCG
GACCCCGGGGCCGCCCTCCTCCTCCCCGAAGACCTGGCCCGGCGGTACGGGGTCTTCCCCCACCATGTGGAAAACGGCAA
GACCCTGGTTCTCCTTATGAAAGACCCCAGGAACATCCTAGCCCTGGACGACGTGCGCTTGGCCCTCAAGCGCAAGGGCC
TGGCCTATGAGGTGGCGCCCGCGGTGGCCACGGAGGCCGCCATCACCAAGCTCATCGAACGCTTTTACGGCAAGGAGGAG
CTGGGCGAGATTGCCAAGGAGCTTTCCAAGGGGTACCAGGAGGAGGAGGCCGTCACCCCTGAGCTGGACGAAAGCGCCGC
CCAGAAGTTCGTCAAGCAGGTGATCCGGGAGGCCTACCTGCAGGATGCCTCCGACATCCACATCGAACCCCGGCAGTTCG
ATGTGCTGGTGCGCCTCCGCATCGACGGCACCCTGCGCCAGTACACCACCCTGTCCAAGGGGGCCCTAAACTCCATTATC
AGCGTGGTCAAGATCATGGGCGGCCTCAACATCGCGGAAAGGCGCCTGCCCCAGGACGGCCGGGTGCGTTACCGGGAAGG
GGCCATCGACGTGGACCTGCGCCTCTCCACCCTGCCCACGGTCTACGGGGAAAAGGCGGTGATGCGCCTTCTGAAGAAGG
CCGCCGACATCCCCGAGATCGAAGGCCTGGGCTTCGCCCCCGGGGTCTTTGAGCGCTTCCAGGAGGTGATCCAAAAGCCT
TATGGCATCTTCCTCATCACCGGGCCCACCGGAAGCGGCAAAAGCTTTACCACCTTCTCCATCCTCAAGCGCATCGCCAC
CCCCGACAAGAACACCCAGACCATCGAGGATCCCGTGGAGTACGAGATCCCCGGCATCAACCAGACCCAGGTGAACCCGC
AGGCGGGGCTCACCTTCGCCCGGGCCCTCAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGG
GACTCGGAAACCGCCAAGATCGCCACCGAGGCCGCCCTGACCGGCCACCTGGTCATCGCCACCCTACACACCAACGACGC
CGCCCAAGCCATCACCCGCCTGGACGAGATGGGGGTGGAGCTCTTCAACATCTCCGCCGCCCTCATCGGGGTTCTTTCCC
AGCGGCTGGTGCGGAGGATCTGCGACCACTGCAAGGTGGAGGTGAGACCCGACCCCGAGGTCCTGCGGCGCCTGGGCCTT
TCCCAGGAGGAGATCCAAGGGGCCAAGCTCTACAAGGGGATGGGATGCGAGCGCTGCGGGGGCACCGGCTACAAGGGACG
CTACGCCATCCACGAACTCCTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCCGGCAAGTCGGCCACCGAGATCA
AGGAGATCGCCAGGAAAAAGGGTATGAAAACCCTGCGGGAAGACGGCATCTACAAGGCCTTCCAGGGAATCACCACCCTG
GAAGAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (173-303)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-480)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (45-148)

• Type II/IV secretion system protein (T2SSE) (513-779)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	87.191	100	0.873
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.229	100	0.564