Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ECO103_RS18585 Genome accession   NC_013353
Coordinates   3607769..3608749 (-) Length   326 a.a.
NCBI ID   WP_001055631.1    Uniprot ID   A0A3E1VZY4
Organism   Escherichia coli O103:H2 str. 12009     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3602769..3613749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECO103_RS18555 (ECO103_3524) yggI 3603419..3603916 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  ECO103_RS18560 (ECO103_3525) endA 3604011..3604718 (+) 708 WP_000286500.1 deoxyribonuclease I -
  ECO103_RS18565 (ECO103_3526) rsmE 3604798..3605529 (+) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ECO103_RS18570 (ECO103_3527) gshB 3605542..3606492 (+) 951 WP_000593273.1 glutathione synthase -
  ECO103_RS18575 (ECO103_3528) yqgE 3606601..3607164 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  ECO103_RS18580 (ECO103_3529) ruvX 3607164..3607580 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  ECO103_RS18585 (ECO103_3530) pilT 3607769..3608749 (-) 981 WP_001055631.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ECO103_RS18590 (ECO103_3531) yggS 3608767..3609471 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  ECO103_RS18595 (ECO103_3532) yggT 3609489..3610055 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  ECO103_RS18600 (ECO103_3533) yggU 3610052..3610342 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  ECO103_RS18605 (ECO103_3534) rdgB 3610350..3610943 (+) 594 WP_001174735.1 XTP/dITP diphosphatase -
  ECO103_RS18610 (ECO103_3535) hemW 3610936..3612072 (+) 1137 WP_000239917.1 radical SAM family heme chaperone HemW -
  ECO103_RS18615 (ECO103_3536) - 3612384..3613370 (+) 987 WP_000784013.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35946.11 Da        Isoelectric Point: 5.7980

>NTDB_id=35347 ECO103_RS18585 WP_001055631.1 3607769..3608749(-) (pilT) [Escherichia coli O103:H2 str. 12009]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=35347 ECO103_RS18585 WP_001055631.1 3607769..3608749(-) (pilT) [Escherichia coli O103:H2 str. 12009]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1VZY4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362

Multiple sequence alignment