Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E3U44_RS03340 Genome accession   NZ_CP038033
Coordinates   682993..684030 (-) Length   345 a.a.
NCBI ID   WP_134356660.1    Uniprot ID   A0A4P7BUM4
Organism   Nitrosococcus wardiae strain D1FHS     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 677993..689030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3U44_RS03320 (E3U44_03320) - 679320..679703 (-) 384 WP_134356656.1 hypothetical protein -
  E3U44_RS03325 (E3U44_03325) - 679848..680528 (-) 681 WP_134356657.1 hypothetical protein -
  E3U44_RS03330 (E3U44_03330) - 680968..681780 (+) 813 WP_134356658.1 SDR family NAD(P)-dependent oxidoreductase -
  E3U44_RS03335 (E3U44_03335) pilU 681847..682983 (-) 1137 WP_134356659.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E3U44_RS03340 (E3U44_03340) pilT 682993..684030 (-) 1038 WP_134356660.1 type IV pilus twitching motility protein PilT Machinery gene
  E3U44_RS03345 (E3U44_03345) - 684151..684846 (+) 696 WP_134356661.1 YggS family pyridoxal phosphate-dependent enzyme -
  E3U44_RS03350 (E3U44_03350) proC 684941..685774 (+) 834 WP_134356662.1 pyrroline-5-carboxylate reductase -
  E3U44_RS03355 (E3U44_03355) - 685767..686330 (+) 564 WP_134356663.1 YggT family protein -
  E3U44_RS03360 (E3U44_03360) - 686337..686648 (+) 312 WP_134356664.1 DUF167 domain-containing protein -
  E3U44_RS03365 (E3U44_03365) - 687012..688235 (-) 1224 WP_134356665.1 AmpG family muropeptide MFS transporter -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38366.05 Da        Isoelectric Point: 6.5806

>NTDB_id=351647 E3U44_RS03340 WP_134356660.1 682993..684030(-) (pilT) [Nitrosococcus wardiae strain D1FHS]
MDIAELLAFSVKNSASDLHISAGLPPMIRVDGDVRRINLPPMEHKEVHAMIYDIMNDKQRKDYEEFLETDFSFEIPGLAR
FRVNAFNQNRGAAAVFRTIPSQVLTLEELDAPAVFKDIANHPRGIVLVTGPTGSGKSTTLAAMVNYKNENDFAHILTIED
PIEFVHESKKSLINQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMVRSMLSESLRAVISQTLLKKFGGGRAAAHEIMIGTPAIRNLIREDKIPQMYSAIQTGQGAGMQTLDQCL
IELARRNVVTKQEAVKKAVNKELFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=351647 E3U44_RS03340 WP_134356660.1 682993..684030(-) (pilT) [Nitrosococcus wardiae strain D1FHS]
ATGGATATCGCTGAACTGCTTGCCTTTAGTGTCAAGAATTCTGCCTCCGATTTACATATCTCAGCCGGACTCCCTCCGAT
GATTCGGGTAGACGGCGACGTGCGCCGGATCAATTTACCGCCCATGGAACACAAGGAAGTTCATGCCATGATTTACGATA
TTATGAATGACAAGCAGCGTAAGGATTATGAAGAATTTCTTGAAACCGATTTTTCCTTCGAGATCCCAGGATTAGCCCGT
TTCCGCGTGAATGCATTCAATCAAAACCGTGGAGCAGCAGCGGTATTTCGTACCATTCCCTCCCAGGTGTTGACTCTTGA
GGAACTCGATGCCCCGGCCGTGTTTAAGGATATCGCCAACCATCCCCGGGGAATTGTGTTGGTGACAGGACCTACAGGCT
CAGGAAAGTCAACTACCTTAGCGGCCATGGTTAATTATAAAAACGAGAATGATTTTGCCCACATTTTAACTATCGAAGAT
CCCATTGAGTTTGTTCATGAAAGCAAAAAAAGCTTAATTAATCAACGTGAAGTCCACCGGGATACCCATGGTTTTAGCGA
AGCGCTACGCTCGGCATTACGTGAAGACCCTGACATCATCCTGGTGGGGGAAATGCGGGATCTAGAAACCATTCGCTTAG
CTTTAACGGCGGCAGAGACGGGCCACTTGGTCTTTGGCACTTTGCATACCAGTTCTGCGGCAAAGACTATTGACCGTATT
ATCGATGTATTCCCTGCCGCAGAGAAAGATATGGTGCGCTCAATGCTCTCCGAATCTCTACGAGCTGTTATTTCTCAGAC
CCTCCTTAAAAAATTTGGCGGTGGTCGGGCGGCTGCCCATGAGATCATGATTGGCACCCCAGCCATCCGTAACCTCATTC
GTGAAGATAAGATCCCTCAAATGTATTCTGCTATCCAAACGGGTCAAGGAGCAGGCATGCAAACCTTGGATCAGTGTCTG
ATAGAGTTAGCCCGCCGCAATGTGGTGACCAAGCAGGAGGCGGTAAAAAAAGCAGTAAATAAAGAACTTTTCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7BUM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

79.942

99.71

0.797
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.198

99.71

0.78
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.81

97.681

0.701
  pilT Vibrio cholerae strain A1552

71.81

97.681

0.701
  pilT Neisseria meningitidis 8013

69.679

99.42

0.693
  pilT Neisseria gonorrhoeae MS11

69.388

99.42

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Vibrio cholerae strain A1552

39.823

98.261

0.391
  pilU Acinetobacter baylyi ADP1

39.118

98.551

0.386

Multiple sequence alignment