Detailed information NTG0351646

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: E3U44_RS03335
• Genome accession: NZ_CP038033
• Coordinates: 681847..682983 (-)
• Length: 378 a.a.
• NCBI ID: WP_134356659.1
• Uniprot ID: A0A4P7BWG3
• Organism: Nitrosococcus wardiae strain D1FHS
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 676847..687983

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E3U44_RS03315 (E3U44_03315)	-	677522..678763 (-)	1242	WP_134356655.1	cation:proton antiporter	-
E3U44_RS03320 (E3U44_03320)	-	679320..679703 (-)	384	WP_134356656.1	hypothetical protein	-
E3U44_RS03325 (E3U44_03325)	-	679848..680528 (-)	681	WP_134356657.1	hypothetical protein	-
E3U44_RS03330 (E3U44_03330)	-	680968..681780 (+)	813	WP_134356658.1	SDR family NAD(P)-dependent oxidoreductase	-
E3U44_RS03335 (E3U44_03335)	pilU	681847..682983 (-)	1137	WP_134356659.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
E3U44_RS03340 (E3U44_03340)	pilT	682993..684030 (-)	1038	WP_134356660.1	type IV pilus twitching motility protein PilT	Machinery gene
E3U44_RS03345 (E3U44_03345)	-	684151..684846 (+)	696	WP_134356661.1	YggS family pyridoxal phosphate-dependent enzyme	-
E3U44_RS03350 (E3U44_03350)	proC	684941..685774 (+)	834	WP_134356662.1	pyrroline-5-carboxylate reductase	-
E3U44_RS03355 (E3U44_03355)	-	685767..686330 (+)	564	WP_134356663.1	YggT family protein	-
E3U44_RS03360 (E3U44_03360)	-	686337..686648 (+)	312	WP_134356664.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 378 a.a.
• Molecular weight: 41738.83 Da
• Isoelectric Point: 6.9714

>NTDB_id=351646 E3U44_RS03335 WP_134356659.1 681847..682983(-) (pilU) [Nitrosococcus wardiae strain D1FHS]
MTDPATELEALLKAMVKHDASDLFITAGFPPSFKIHGKVMPASQRPLSESQAKELVFTAMTSQQQQEFSQTHEGNFALGR
ENLGRFRVNAFYQRSSAGMVLRRIKTEIPSFEQIHLPATLCQLTMISRGMVIFVGGTGSGKSTSLAAMVGYRNRNSKGHI
VTIEDPIEYIHEHEGCIITQREVGIDTQSYEVALKNTLRQAPDVILIGEIRSRETMEYAMAFAETGHLCLSTLHANNANQ
ALERIINFFPEDRRRQLLLDLSLNLKAVVAQRLIPTADGKGRRAAVEILINTPLIAELIYKGDVVGIKDLMKRSTEQGMQ
SFDKAVYQLYKDGAISYEDALRNADSANEVRLMVKLGGEGAAQKLSAAIEGVTLQGPE

Nucleotide

• Length: 1137 bp

>NTDB_id=351646 E3U44_RS03335 WP_134356659.1 681847..682983(-) (pilU) [Nitrosococcus wardiae strain D1FHS]
ATGACAGACCCAGCAACAGAACTAGAGGCTCTGCTAAAGGCCATGGTCAAGCACGATGCCTCGGATCTCTTTATTACGGC
AGGATTTCCCCCTAGTTTCAAGATCCACGGTAAAGTTATGCCAGCTAGCCAGCGGCCTTTGTCGGAAAGTCAAGCAAAGG
AGTTGGTATTCACAGCGATGACTTCCCAGCAGCAGCAGGAGTTTAGCCAAACCCATGAGGGAAATTTTGCCCTTGGCAGA
GAAAATCTCGGTCGGTTTCGTGTCAATGCCTTCTACCAACGGAGTAGTGCAGGTATGGTGCTGCGCCGCATCAAAACAGA
GATCCCCAGCTTTGAGCAGATCCACTTGCCCGCGACATTATGTCAACTGACCATGATTAGCCGCGGTATGGTGATCTTTG
TAGGGGGAACAGGGTCGGGTAAATCCACCTCCCTTGCCGCCATGGTAGGTTACCGTAATCGTAATAGCAAGGGCCATATT
GTGACTATTGAAGATCCCATTGAGTATATCCATGAGCATGAAGGCTGTATCATCACCCAGCGGGAAGTGGGTATCGATAC
TCAGTCTTACGAAGTGGCGCTTAAGAATACGTTACGGCAAGCTCCAGATGTGATCTTGATTGGTGAGATTCGGAGTCGGG
AAACCATGGAATATGCTATGGCCTTTGCCGAAACAGGTCATCTGTGTTTATCAACCCTTCATGCGAATAATGCTAATCAG
GCTTTAGAACGCATTATCAATTTCTTCCCTGAAGATCGACGCCGGCAATTGCTGTTGGATCTTTCACTGAATCTTAAGGC
TGTGGTTGCCCAGCGCCTGATTCCGACCGCAGATGGTAAGGGACGTCGGGCTGCAGTAGAGATCCTTATTAATACGCCAC
TAATTGCTGAGCTTATTTACAAGGGGGATGTTGTAGGTATCAAGGATTTGATGAAACGCTCGACCGAACAAGGGATGCAA
AGTTTTGATAAAGCTGTTTATCAATTATATAAAGATGGGGCTATTAGTTATGAAGATGCGCTGCGCAATGCCGATTCGGC
AAACGAAGTGCGTCTAATGGTAAAGCTAGGGGGTGAGGGAGCGGCCCAGAAATTAAGCGCTGCCATCGAAGGGGTTACTC
TTCAGGGACCAGAGTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (12-275)

3D structure

• AlphaFold DB: A0A4P7BWG3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	63.957	97.619	0.624
pilU	Acinetobacter baylyi ADP1	62.254	93.915	0.585
pilU	Vibrio cholerae strain A1552	56.098	97.619	0.548
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.108	90.741	0.373