Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CAP2UW1_RS17855 Genome accession   NC_013194
Coordinates   4109229..4110275 (+) Length   348 a.a.
NCBI ID   WP_015768027.1    Uniprot ID   A0A9D8PVF2
Organism   Candidatus Accumulibacter phosphatis     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4104229..4115275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAP2UW1_RS17825 (CAP2UW1_3595) - 4104588..4105547 (+) 960 WP_015768021.1 aspartate carbamoyltransferase catalytic subunit -
  CAP2UW1_RS17830 (CAP2UW1_3596) - 4105551..4106807 (+) 1257 WP_015768022.1 dihydroorotase -
  CAP2UW1_RS17835 (CAP2UW1_3597) - 4106825..4107115 (-) 291 WP_015768023.1 DUF167 domain-containing protein -
  CAP2UW1_RS17840 (CAP2UW1_3598) - 4107119..4107658 (-) 540 WP_015768024.1 YggT family protein -
  CAP2UW1_RS17845 (CAP2UW1_3599) proC 4107658..4108473 (-) 816 WP_015768025.1 pyrroline-5-carboxylate reductase -
  CAP2UW1_RS17850 (CAP2UW1_3600) - 4108470..4109159 (-) 690 WP_015768026.1 YggS family pyridoxal phosphate-dependent enzyme -
  CAP2UW1_RS17855 (CAP2UW1_3601) pilT 4109229..4110275 (+) 1047 WP_015768027.1 type IV pilus twitching motility protein PilT Machinery gene
  CAP2UW1_RS17860 (CAP2UW1_3602) pilU 4110303..4111442 (+) 1140 WP_015768028.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CAP2UW1_RS17865 (CAP2UW1_3603) - 4111530..4112714 (-) 1185 WP_015768029.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  CAP2UW1_RS17870 (CAP2UW1_3604) - 4112736..4113152 (-) 417 WP_015768030.1 CopD family protein -
  CAP2UW1_RS17875 (CAP2UW1_3605) - 4113146..4114051 (-) 906 WP_015768031.1 SDR family oxidoreductase -
  CAP2UW1_RS17880 (CAP2UW1_3606) - 4114091..4115164 (+) 1074 WP_015768032.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38637.44 Da        Isoelectric Point: 7.3196

>NTDB_id=35158 CAP2UW1_RS17855 WP_015768027.1 4109229..4110275(+) (pilT) [Candidatus Accumulibacter phosphatis]
MDITELLAFSVKNKASDLHLSSGLPPMIRVNGDVRRINLPAMEHKDVHGMVYDIMNDGQRKHYEDTLECDFSFEIPNLAR
FRVNAFVQNRGAAAVFRTIPSKVLTLEDLNCPRIFKDISEYPRGIVLVTGPTGSGKSTTLAAMINHVNETMYGHILTVED
PIEFVHQSKKCLINQREIGPHTLSFQNALRSALREDPDVILVGEMRDLETIRLALSGAETGHLVFATLHTSSAAKTIDRI
IDVFPAAEKEMVRSMLSESIRAVISQTLLKTKDGQGRIAAHEILIGTPAIRNLIRENKIAQMYSTLQTGQQYGMQTLDHN
LLELVKRNLVAASEARSKAANKDVFPGA

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=35158 CAP2UW1_RS17855 WP_015768027.1 4109229..4110275(+) (pilT) [Candidatus Accumulibacter phosphatis]
ATGGACATCACGGAACTGCTCGCCTTCAGCGTCAAGAACAAGGCTTCCGACCTGCACCTGTCATCCGGGTTACCGCCGAT
GATCCGCGTGAATGGCGATGTGCGCCGCATCAACCTGCCAGCGATGGAACACAAGGACGTGCACGGCATGGTGTACGACA
TCATGAACGACGGCCAGCGCAAGCACTACGAAGACACGCTCGAATGCGACTTCTCGTTCGAGATTCCCAACCTCGCGCGC
TTTCGCGTCAACGCCTTCGTCCAGAACCGCGGCGCTGCTGCCGTATTCCGGACCATTCCGTCAAAGGTACTGACGCTCGA
AGATCTCAACTGCCCGAGGATCTTCAAGGACATCTCGGAATACCCGCGCGGGATTGTGCTGGTCACCGGACCGACGGGGT
CGGGCAAGTCAACAACCCTGGCGGCAATGATCAATCACGTCAATGAGACGATGTATGGCCACATTCTGACGGTCGAGGAT
CCGATCGAGTTTGTGCACCAGTCGAAGAAATGCCTGATCAACCAGCGCGAGATCGGCCCCCACACCCTGTCGTTCCAGAA
TGCGCTGCGCTCGGCCCTGCGCGAGGACCCGGACGTCATCCTGGTTGGCGAGATGCGCGATCTGGAAACGATCCGTCTGG
CGCTCTCCGGTGCCGAGACCGGCCACCTAGTCTTCGCCACCCTGCACACTTCCAGCGCTGCGAAGACGATCGACCGGATC
ATCGACGTGTTTCCTGCTGCCGAGAAGGAGATGGTCCGATCGATGTTGTCCGAGTCGATTCGCGCCGTGATCTCGCAAAC
GCTGCTCAAGACCAAGGACGGTCAGGGGCGAATCGCGGCGCACGAAATCCTCATCGGCACGCCAGCGATCCGCAACCTGA
TCCGCGAAAACAAGATCGCCCAGATGTATTCCACCCTGCAGACTGGCCAGCAGTATGGCATGCAGACCCTCGACCACAAC
CTGCTCGAGCTCGTCAAGCGAAATCTCGTTGCGGCAAGCGAGGCTCGCAGCAAGGCGGCCAACAAGGACGTCTTCCCCGG
CGCTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

75.942

99.138

0.753
  pilT Acinetobacter baumannii strain A118

75.942

99.138

0.753
  pilT Acinetobacter nosocomialis M2

75.942

99.138

0.753
  pilT Pseudomonas stutzeri DSM 10701

73.333

99.138

0.727
  pilT Acinetobacter baylyi ADP1

72.754

99.138

0.721
  pilT Pseudomonas aeruginosa PAK

73.746

97.414

0.718
  pilT Neisseria meningitidis 8013

72.303

98.563

0.713
  pilT Neisseria gonorrhoeae MS11

72.012

98.563

0.71
  pilT Legionella pneumophila strain ERS1305867

71.014

99.138

0.704
  pilT Legionella pneumophila strain Lp02

71.014

99.138

0.704
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.456

97.126

0.655
  pilT Vibrio cholerae strain A1552

67.456

97.126

0.655
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

99.138

0.491
  pilU Acinetobacter baylyi ADP1

40.882

97.701

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.264

0.394
  pilU Vibrio cholerae strain A1552

40

96.264

0.385

Multiple sequence alignment