Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CAP2UW1_RS17860 Genome accession   NC_013194
Coordinates   4110303..4111442 (+) Length   379 a.a.
NCBI ID   WP_015768028.1    Uniprot ID   A0A9D8PRQ8
Organism   Candidatus Accumulibacter phosphatis     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4105303..4116442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAP2UW1_RS17830 (CAP2UW1_3596) - 4105551..4106807 (+) 1257 WP_015768022.1 dihydroorotase -
  CAP2UW1_RS17835 (CAP2UW1_3597) - 4106825..4107115 (-) 291 WP_015768023.1 DUF167 domain-containing protein -
  CAP2UW1_RS17840 (CAP2UW1_3598) - 4107119..4107658 (-) 540 WP_015768024.1 YggT family protein -
  CAP2UW1_RS17845 (CAP2UW1_3599) proC 4107658..4108473 (-) 816 WP_015768025.1 pyrroline-5-carboxylate reductase -
  CAP2UW1_RS17850 (CAP2UW1_3600) - 4108470..4109159 (-) 690 WP_015768026.1 YggS family pyridoxal phosphate-dependent enzyme -
  CAP2UW1_RS17855 (CAP2UW1_3601) pilT 4109229..4110275 (+) 1047 WP_015768027.1 type IV pilus twitching motility protein PilT Machinery gene
  CAP2UW1_RS17860 (CAP2UW1_3602) pilU 4110303..4111442 (+) 1140 WP_015768028.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CAP2UW1_RS17865 (CAP2UW1_3603) - 4111530..4112714 (-) 1185 WP_015768029.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  CAP2UW1_RS17870 (CAP2UW1_3604) - 4112736..4113152 (-) 417 WP_015768030.1 CopD family protein -
  CAP2UW1_RS17875 (CAP2UW1_3605) - 4113146..4114051 (-) 906 WP_015768031.1 SDR family oxidoreductase -
  CAP2UW1_RS17880 (CAP2UW1_3606) - 4114091..4115164 (+) 1074 WP_015768032.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  CAP2UW1_RS17885 (CAP2UW1_3607) - 4115157..4116332 (+) 1176 WP_015768033.1 EAL and HDOD domain-containing protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42608.02 Da        Isoelectric Point: 7.0409

>NTDB_id=35159 CAP2UW1_RS17860 WP_015768028.1 4110303..4111442(+) (pilU) [Candidatus Accumulibacter phosphatis]
MEHDQALKFMHELLRLMTQKNGSDLFITAHFPPAIKIDGKVMPVSNQPLLPQHSAELARSIMNDRQAAEFEEKRECNFAI
SPTGIGRFRVNALVQQGRVAVVCRTINLVVPTLDELELPPVLKDISLAARGLVIFVGGTGTGKTTSLAALVDHRNRNSHG
HIITIEDPIEFVHEHKKCIVTQREVGVDTDSWETALKNTLRQAPNVIMMGEIRDRQTMDYAIAFAETGHLCLATLHANNT
NQAIDRVINFFPEERRHQLLMDLSLNLRAFVSQRLLPKKDGKGRVPAIEVMLNSPLVSDLVFKGQVHEIKEVMKRSRELG
MQTFDQALFDLYEGGQIRYEDALRNADSLNDLRLQIKLHGKESKDRDLSAGIGHLDIVQ

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=35159 CAP2UW1_RS17860 WP_015768028.1 4110303..4111442(+) (pilU) [Candidatus Accumulibacter phosphatis]
ATGGAACACGATCAAGCACTCAAGTTCATGCACGAGTTGCTGCGTCTGATGACGCAGAAGAATGGGTCGGACCTCTTCAT
CACGGCCCATTTTCCACCGGCCATCAAGATTGACGGCAAGGTGATGCCGGTGTCCAACCAACCGCTCCTCCCCCAGCACT
CCGCGGAACTGGCGCGCTCGATCATGAACGACCGTCAGGCCGCCGAGTTCGAAGAAAAGAGGGAATGCAACTTTGCCATC
TCGCCTACCGGGATTGGCCGTTTTCGCGTCAATGCATTGGTACAGCAGGGGCGCGTCGCGGTGGTCTGCCGGACGATCAA
CCTGGTGGTACCGACCCTTGACGAACTCGAATTGCCACCGGTACTCAAGGACATTTCGCTTGCCGCGCGCGGTCTGGTGA
TTTTTGTCGGCGGCACGGGCACCGGCAAGACCACGTCTTTGGCCGCACTCGTCGATCACCGCAACCGTAACAGCCACGGT
CATATCATCACGATCGAGGATCCCATCGAGTTCGTTCACGAGCACAAGAAGTGCATCGTCACTCAGCGCGAGGTCGGCGT
GGACACCGACTCCTGGGAGACAGCACTGAAGAATACGCTACGTCAGGCGCCGAACGTGATCATGATGGGAGAGATTCGTG
ATCGCCAGACCATGGACTACGCCATCGCCTTCGCCGAAACCGGACACTTGTGCCTGGCCACGCTGCACGCCAACAACACC
AACCAGGCCATCGACCGGGTCATCAACTTCTTCCCCGAGGAACGGCGCCATCAGTTGCTGATGGATCTTTCTCTCAACCT
GCGTGCGTTCGTCTCCCAGCGCCTGCTGCCGAAGAAGGATGGCAAGGGAAGGGTGCCGGCGATTGAGGTCATGCTCAATT
CACCACTCGTTTCCGACCTCGTTTTCAAGGGTCAGGTGCATGAGATCAAGGAAGTGATGAAGCGATCGCGCGAACTGGGT
ATGCAGACTTTCGACCAGGCGCTGTTCGATCTCTACGAGGGCGGGCAGATCAGGTACGAGGATGCCCTGCGCAACGCCGA
CTCGCTGAATGACCTGCGCCTGCAGATCAAGCTGCATGGCAAGGAATCGAAAGACCGGGATCTCAGCGCCGGCATCGGTC
ATCTGGATATCGTCCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(127-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.117

94.723

0.588
  pilU Acinetobacter baylyi ADP1

56.546

94.723

0.536
  pilU Vibrio cholerae strain A1552

51.724

91.821

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.179

88.391

0.391
  pilT Pseudomonas aeruginosa PAK

42.388

88.391

0.375
  pilT Acinetobacter baylyi ADP1

41.543

88.918

0.369
  pilT Pseudomonas stutzeri DSM 10701

41.194

88.391

0.364
  pilT Acinetobacter baumannii D1279779

41.39

87.335

0.361
  pilT Acinetobacter baumannii strain A118

41.39

87.335

0.361
  pilT Acinetobacter nosocomialis M2

41.39

87.335

0.361

Multiple sequence alignment