Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KKOR_RS01070 Genome accession   NC_013166
Coordinates   222654..223688 (+) Length   344 a.a.
NCBI ID   WP_012800154.1    Uniprot ID   C7R6X3
Organism   Kangiella koreensis DSM 16069     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 217654..228688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KKOR_RS01040 (Kkor_0212) hemW 217760..218992 (-) 1233 WP_012800148.1 radical SAM family heme chaperone HemW -
  KKOR_RS01045 (Kkor_0213) rdgB 218998..219630 (-) 633 WP_012800149.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  KKOR_RS01050 (Kkor_0214) - 219737..220198 (-) 462 WP_012800150.1 DUF4426 domain-containing protein -
  KKOR_RS01055 (Kkor_0215) - 220355..220942 (-) 588 WP_012800151.1 YggT family protein -
  KKOR_RS01060 (Kkor_0216) proC 220964..221821 (-) 858 WP_012800152.1 pyrroline-5-carboxylate reductase -
  KKOR_RS01065 (Kkor_0217) - 221932..222615 (-) 684 WP_012800153.1 YggS family pyridoxal phosphate-dependent enzyme -
  KKOR_RS01070 (Kkor_0218) pilT 222654..223688 (+) 1035 WP_012800154.1 type IV pilus twitching motility protein PilT Machinery gene
  KKOR_RS01075 (Kkor_0219) pilU 223698..224837 (+) 1140 WP_012800155.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KKOR_RS01080 (Kkor_0220) - 224882..227143 (-) 2262 WP_012800156.1 EAL domain-containing protein -
  KKOR_RS01085 (Kkor_0221) ruvX 227257..227694 (-) 438 WP_012800157.1 Holliday junction resolvase RuvX -
  KKOR_RS01090 (Kkor_0222) - 227694..228257 (-) 564 WP_012800158.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38282.88 Da        Isoelectric Point: 6.7305

>NTDB_id=35076 KKOR_RS01070 WP_012800154.1 222654..223688(+) (pilT) [Kangiella koreensis DSM 16069]
MDITELLAFSVKNNASDLHISAGMPPLIRVDGDIRRINVPPFEDKEVHTLIYDIMNDKQRKDYEEFLEADFSFELKDLAR
FRVNAFVQNRGRAAVFRTIPSRVLTLEELNAPKIFQDISLEPRGLVLVTGPTGSGKSTTLAGMIDYVNENKHDHILTIED
PIEFVHESKKCLVNQREVHRDTHSFSNALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKAMVRSMLSESLQAVIAQTLLKKNGGGRVAAHEILVGTPAIRNLIREDKVAQMYSAMQTGLSLGMQTLDHSL
KQLVSKGVVSRTDARAKAKSKEDF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=35076 KKOR_RS01070 WP_012800154.1 222654..223688(+) (pilT) [Kangiella koreensis DSM 16069]
ATGGATATTACAGAGCTTTTAGCTTTCAGTGTAAAGAATAATGCATCGGATTTGCATATTTCCGCGGGTATGCCGCCTCT
TATTCGTGTCGATGGTGACATTCGTCGTATCAATGTACCGCCATTTGAAGACAAGGAAGTGCACACGTTAATCTACGATA
TCATGAATGATAAGCAGCGAAAGGATTATGAAGAATTCCTTGAGGCCGACTTCTCGTTTGAATTAAAAGATTTGGCACGT
TTCCGTGTGAATGCCTTTGTACAAAATCGTGGCCGTGCCGCCGTTTTTCGTACAATTCCTTCTCGCGTCTTAACTTTAGA
AGAGTTAAATGCGCCAAAAATTTTCCAGGATATCTCATTAGAGCCACGCGGACTGGTTTTGGTTACGGGACCAACGGGCT
CCGGTAAATCAACAACGCTGGCCGGTATGATCGATTATGTGAATGAAAATAAGCATGATCACATTCTAACCATTGAGGAC
CCCATCGAGTTCGTACACGAAAGTAAGAAATGTCTGGTTAACCAGCGTGAGGTTCATCGTGATACGCACAGCTTCTCTAA
CGCATTGCGTTCAGCGCTTCGTGAAGATCCCGATATTATTCTGGTCGGTGAGATGCGTGACCTTGAAACTATTCGCTTAG
CGCTAACAGCGGCAGAAACTGGTCACTTGGTATTTGGTACCCTGCATACAACCTCTGCGGCTAAAACGATTGACCGTGTG
GTTGACGTATTCCCTGCAGGTGAAAAGGCGATGGTTCGCTCAATGTTATCAGAATCATTACAGGCGGTTATTGCGCAAAC
GCTATTGAAGAAAAATGGTGGTGGCCGTGTGGCAGCCCATGAAATCTTGGTGGGTACCCCAGCTATTCGAAACCTGATTC
GTGAAGATAAGGTCGCGCAAATGTACTCTGCGATGCAGACAGGTTTGTCACTTGGTATGCAGACGCTGGATCACAGCTTA
AAGCAGTTGGTCAGCAAGGGTGTTGTGTCGCGCACTGATGCTCGTGCAAAAGCTAAGAGCAAAGAAGATTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C7R6X3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.395

100

0.814
  pilT Pseudomonas stutzeri DSM 10701

79.651

100

0.797
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

72.965

100

0.73
  pilT Legionella pneumophila strain ERS1305867

72.965

100

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

97.965

0.689
  pilT Vibrio cholerae strain A1552

70.326

97.965

0.689
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Vibrio cholerae strain A1552

42.609

100

0.427
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

39.181

99.419

0.39

Multiple sequence alignment