Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KKOR_RS01075 Genome accession   NC_013166
Coordinates   223698..224837 (+) Length   379 a.a.
NCBI ID   WP_012800155.1    Uniprot ID   -
Organism   Kangiella koreensis DSM 16069     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 218698..229837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KKOR_RS01045 (Kkor_0213) rdgB 218998..219630 (-) 633 WP_012800149.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  KKOR_RS01050 (Kkor_0214) - 219737..220198 (-) 462 WP_012800150.1 DUF4426 domain-containing protein -
  KKOR_RS01055 (Kkor_0215) - 220355..220942 (-) 588 WP_012800151.1 YggT family protein -
  KKOR_RS01060 (Kkor_0216) proC 220964..221821 (-) 858 WP_012800152.1 pyrroline-5-carboxylate reductase -
  KKOR_RS01065 (Kkor_0217) - 221932..222615 (-) 684 WP_012800153.1 YggS family pyridoxal phosphate-dependent enzyme -
  KKOR_RS01070 (Kkor_0218) pilT 222654..223688 (+) 1035 WP_012800154.1 type IV pilus twitching motility protein PilT Machinery gene
  KKOR_RS01075 (Kkor_0219) pilU 223698..224837 (+) 1140 WP_012800155.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KKOR_RS01080 (Kkor_0220) - 224882..227143 (-) 2262 WP_012800156.1 EAL domain-containing protein -
  KKOR_RS01085 (Kkor_0221) ruvX 227257..227694 (-) 438 WP_012800157.1 Holliday junction resolvase RuvX -
  KKOR_RS01090 (Kkor_0222) - 227694..228257 (-) 564 WP_012800158.1 YqgE/AlgH family protein -
  KKOR_RS01095 (Kkor_0223) - 228306..229229 (-) 924 WP_143715028.1 energy transducer TonB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42668.14 Da        Isoelectric Point: 6.6903

>NTDB_id=35077 KKOR_RS01075 WP_012800155.1 223698..224837(+) (pilU) [Kangiella koreensis DSM 16069]
MDINPLLKALMEHKGSDLFITVHRPPCIKINGQIKPVTKSMLTPAQVEALIHSSMNEKQMKELEEKKECQYAISVENIGR
FRASAFYQRNQPGLVVRRIETKIPTTEELKIPSILNDLVMKKRGIIIFVGATGTGKSTSLAAMIGYRNRNSAGHIISIED
PIEYVHRHDQCIITQREVGIDTESFETALKNTLRQAPDVILIGEVRSKETMANAITFAETGHLCLCTLHANNANQAIDRI
LHFFPKEEHSRVLMDLSLNTRAIIAQQLVPTKDGKGRRAAIEILINSPLISDHIRKGEVHMLKELMARSRELGMQTFDQA
LFDLYVEGEINYDDALLYADSANDLRLKIKLHEKDADSLSEGTEGLQVEMDEDRGGRMF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=35077 KKOR_RS01075 WP_012800155.1 223698..224837(+) (pilU) [Kangiella koreensis DSM 16069]
ATGGATATTAATCCACTATTAAAAGCACTAATGGAGCACAAGGGTTCGGATTTGTTTATAACGGTCCACCGTCCCCCTTG
TATTAAAATCAACGGACAGATTAAACCGGTAACTAAAAGTATGCTGACACCAGCTCAGGTTGAAGCCTTGATTCATTCTT
CAATGAATGAAAAGCAAATGAAGGAGCTGGAAGAAAAGAAAGAATGTCAGTATGCGATCAGTGTCGAAAATATTGGTCGT
TTCCGTGCCAGTGCCTTTTATCAACGTAACCAACCTGGTTTAGTGGTTCGCCGAATTGAAACCAAAATCCCGACCACAGA
AGAGCTCAAGATTCCAAGTATATTGAACGATCTGGTCATGAAAAAGCGTGGGATCATCATTTTTGTGGGCGCAACCGGTA
CCGGTAAATCAACTTCCTTAGCAGCGATGATTGGATATCGTAATCGAAACTCTGCTGGCCACATTATCAGTATTGAGGAC
CCGATTGAGTATGTGCATCGTCATGATCAATGCATTATTACTCAGCGCGAAGTAGGGATTGATACAGAGTCGTTTGAGAC
GGCACTGAAAAATACTTTACGCCAGGCACCTGATGTTATTCTGATTGGTGAGGTTCGCTCTAAAGAAACTATGGCCAATG
CAATCACCTTCGCTGAGACTGGTCACTTATGTTTATGTACCTTACACGCCAATAATGCAAACCAGGCGATTGACCGTATT
TTGCACTTCTTCCCCAAAGAAGAACATAGTCGAGTATTAATGGATTTATCGTTAAATACTCGTGCCATTATTGCTCAGCA
ATTGGTTCCTACTAAAGATGGTAAAGGCCGTCGTGCAGCGATTGAGATTCTGATTAACTCACCTTTGATTTCTGATCATA
TTCGTAAAGGTGAAGTTCATATGTTGAAAGAGTTGATGGCTCGTTCAAGAGAGCTTGGTATGCAAACTTTTGACCAAGCA
TTATTTGACTTATATGTCGAAGGTGAAATCAATTATGACGATGCATTGCTGTATGCGGACTCAGCTAATGACTTGCGTCT
TAAAATTAAACTGCATGAAAAAGATGCGGACTCTCTTTCCGAGGGGACAGAGGGCTTGCAGGTGGAAATGGATGAAGACC
GTGGTGGTCGAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.526

100

0.657
  pilU Acinetobacter baylyi ADP1

63.099

93.668

0.591
  pilU Vibrio cholerae strain A1552

55.256

97.889

0.541
  pilT Acinetobacter baylyi ADP1

41.349

89.974

0.372
  pilT Pseudomonas stutzeri DSM 10701

41.493

88.391

0.367
  pilT Acinetobacter baumannii D1279779

41.692

87.335

0.364
  pilT Acinetobacter baumannii strain A118

41.692

87.335

0.364
  pilT Acinetobacter nosocomialis M2

41.692

87.335

0.364
  pilT Pseudomonas aeruginosa PAK

41.194

88.391

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.39

87.335

0.361
  pilT Vibrio cholerae strain A1552

41.39

87.335

0.361

Multiple sequence alignment