Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EKN05_RS00670 Genome accession   NZ_CP037923
Coordinates   138355..139335 (+) Length   326 a.a.
NCBI ID   WP_001055627.1    Uniprot ID   A0A4P7TIP5
Organism   Shigella flexneri 5a str. M90T     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 133355..144335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKN05_RS00640 (EKN05_000640) - 133755..134718 (-) 964 Protein_126 TRAP transporter substrate-binding protein -
  EKN05_RS00645 (EKN05_000645) hemW 135032..136168 (-) 1137 WP_000239914.1 radical SAM family heme chaperone HemW -
  EKN05_RS00650 (EKN05_000650) - 136161..136754 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  EKN05_RS00655 (EKN05_000655) yggU 136762..137052 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  EKN05_RS00660 (EKN05_000660) yggT 137049..137615 (-) 567 WP_001094818.1 osmotic shock tolerance protein YggT -
  EKN05_RS00665 (EKN05_000665) yggS 137633..138337 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  EKN05_RS00670 (EKN05_000670) pilT 138355..139335 (+) 981 WP_001055627.1 type IV pilus twitching motility protein PilT Machinery gene
  EKN05_RS00675 (EKN05_000675) ruvX 139511..139927 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  EKN05_RS00680 (EKN05_000680) - 139927..140490 (-) 564 WP_024260306.1 YqgE/AlgH family protein -
  EKN05_RS00685 (EKN05_000685) gshB 140599..141549 (-) 951 WP_000593267.1 glutathione synthase -
  EKN05_RS00690 (EKN05_000690) rsmE 141562..142293 (-) 732 WP_005105806.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EKN05_RS00695 (EKN05_000695) endA 142373..143080 (-) 708 WP_000286517.1 deoxyribonuclease I -
  EKN05_RS00700 (EKN05_000700) - 143175..143672 (-) 498 WP_005051770.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35942.12 Da        Isoelectric Point: 5.7980

>NTDB_id=350627 EKN05_RS00670 WP_001055627.1 138355..139335(+) (pilT) [Shigella flexneri 5a str. M90T]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=350627 EKN05_RS00670 WP_001055627.1 138355..139335(+) (pilT) [Shigella flexneri 5a str. M90T]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCTACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTTGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7TIP5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362

Multiple sequence alignment