Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EZV72_RS04520 Genome accession   NZ_CP036536
Coordinates   1062373..1063410 (+) Length   345 a.a.
NCBI ID   WP_137166113.1    Uniprot ID   -
Organism   Salinimonas lutimaris strain DPSR-4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1057373..1068410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZV72_RS04490 yaaA 1058026..1058799 (+) 774 WP_137166108.1 peroxide stress protein YaaA -
  EZV72_RS04495 - 1058826..1059455 (+) 630 WP_137166109.1 glutathione S-transferase family protein -
  EZV72_RS04500 - 1059740..1060174 (-) 435 WP_232364508.1 DUF4426 domain-containing protein -
  EZV72_RS04505 - 1060229..1060771 (-) 543 WP_137166110.1 YggT family protein -
  EZV72_RS04510 proC 1060791..1061612 (-) 822 WP_137166111.1 pyrroline-5-carboxylate reductase -
  EZV72_RS04515 - 1061652..1062341 (-) 690 WP_137166112.1 YggS family pyridoxal phosphate-dependent enzyme -
  EZV72_RS04520 pilT 1062373..1063410 (+) 1038 WP_137166113.1 type IV pilus twitching motility protein PilT Machinery gene
  EZV72_RS04525 pilU 1063422..1064528 (+) 1107 WP_137166114.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EZV72_RS04530 - 1064531..1064899 (+) 369 WP_137166115.1 DUF2007 domain-containing protein -
  EZV72_RS04535 ruvX 1064896..1065324 (-) 429 WP_137166116.1 Holliday junction resolvase RuvX -
  EZV72_RS04540 - 1065317..1065883 (-) 567 WP_137166117.1 YqgE/AlgH family protein -
  EZV72_RS04545 gshB 1065888..1066847 (-) 960 WP_137166118.1 glutathione synthase -
  EZV72_RS04550 - 1066960..1067688 (-) 729 WP_137166119.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38349.10 Da        Isoelectric Point: 6.7833

>NTDB_id=349511 EZV72_RS04520 WP_137166113.1 1062373..1063410(+) (pilT) [Salinimonas lutimaris strain DPSR-4]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGQMRRLNIPELDHKQVHGLIYEIMNDAQRKEYEENLEVDFSFEVKDLSR
FRVNAFVQNRGAAAVLRTIPSRVLTLDELGAPEIFKSIINQPTGLVLVTGATGSGKSTTLAAMIDHINAHQRHHILTIED
PIEFVHDNKLSLLNQREVHRDTKSFNNALRSALREDPDVILVGELRDLETIRLAMSAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAAEKSMIRSMLSESLRAVISQTLLKKIGGGRVAAHEIMLGVPAIRNLIREDKVPQMYSVIQTGQAMGMQTMDQCL
QRLVAKGVITQQDAAEKSIDKQNSF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=349511 EZV72_RS04520 WP_137166113.1 1062373..1063410(+) (pilT) [Salinimonas lutimaris strain DPSR-4]
GTGGATATTACCGAACTTTTAGCTTTCAGTGTAAAGAATAACGCCTCGGATTTACACTTGTCAGCAGGCCTGCCGCCTAT
CATCCGGGTTGACGGACAGATGCGCAGGCTGAATATCCCCGAGCTGGATCATAAGCAGGTTCATGGCCTGATTTATGAAA
TTATGAATGATGCCCAGCGCAAGGAGTATGAAGAAAACCTGGAAGTTGACTTTTCCTTTGAAGTGAAAGACTTATCACGT
TTCCGGGTGAATGCATTTGTTCAGAACCGGGGCGCTGCTGCGGTGTTACGTACCATTCCGTCCCGGGTTCTGACCCTGGA
TGAACTGGGCGCACCGGAAATCTTTAAATCGATTATTAACCAGCCCACAGGACTGGTGCTGGTAACCGGCGCCACCGGCT
CAGGTAAAAGTACCACGCTGGCGGCGATGATAGACCATATCAATGCCCATCAGCGTCATCATATTCTGACTATCGAAGAT
CCAATCGAGTTTGTGCACGACAATAAGTTGAGTCTGCTCAACCAGCGTGAAGTGCACCGTGATACCAAGAGCTTTAATAA
CGCGCTGCGCTCGGCGCTGCGTGAAGACCCGGATGTTATTCTGGTGGGTGAATTGCGGGATCTGGAAACCATCCGGCTGG
CGATGTCAGCGGCAGAAACCGGCCATCTGGTATTTGGTACACTGCATACTAACTCGGCGCCGAAAACCATCGACCGGATT
ATTGATGTATTTCCGGCGGCCGAAAAGTCGATGATACGCTCAATGTTGTCAGAGTCTCTGCGGGCGGTTATTTCACAAAC
CCTGCTTAAAAAAATCGGCGGTGGCCGGGTGGCGGCCCATGAGATTATGCTGGGTGTACCGGCGATCCGAAACCTGATTC
GTGAAGATAAGGTTCCGCAGATGTATTCGGTGATTCAGACCGGCCAGGCCATGGGCATGCAAACCATGGATCAGTGTCTG
CAGCGGCTGGTGGCAAAAGGGGTCATTACCCAGCAGGATGCCGCTGAAAAATCGATTGATAAACAAAACTCTTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

74.052

99.42

0.736
  pilT Pseudomonas stutzeri DSM 10701

73.761

99.42

0.733
  pilT Acinetobacter baylyi ADP1

75.684

95.362

0.722
  pilT Acinetobacter baumannii D1279779

74.551

96.812

0.722
  pilT Acinetobacter baumannii strain A118

74.551

96.812

0.722
  pilT Acinetobacter nosocomialis M2

74.251

96.812

0.719
  pilT Legionella pneumophila strain Lp02

71.261

98.841

0.704
  pilT Legionella pneumophila strain ERS1305867

71.261

98.841

0.704
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.81

97.681

0.701
  pilT Vibrio cholerae strain A1552

71.81

97.681

0.701
  pilT Neisseria meningitidis 8013

66.667

100

0.667
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.664
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.353

99.42

0.53
  pilU Vibrio cholerae strain A1552

42.687

97.101

0.414
  pilU Pseudomonas stutzeri DSM 10701

41.888

98.261

0.412
  pilU Acinetobacter baylyi ADP1

40.476

97.391

0.394

Multiple sequence alignment