Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EZV72_RS04525 Genome accession   NZ_CP036536
Coordinates   1063422..1064528 (+) Length   368 a.a.
NCBI ID   WP_137166114.1    Uniprot ID   -
Organism   Salinimonas lutimaris strain DPSR-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1058422..1069528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZV72_RS04495 - 1058826..1059455 (+) 630 WP_137166109.1 glutathione S-transferase family protein -
  EZV72_RS04500 - 1059740..1060174 (-) 435 WP_232364508.1 DUF4426 domain-containing protein -
  EZV72_RS04505 - 1060229..1060771 (-) 543 WP_137166110.1 YggT family protein -
  EZV72_RS04510 proC 1060791..1061612 (-) 822 WP_137166111.1 pyrroline-5-carboxylate reductase -
  EZV72_RS04515 - 1061652..1062341 (-) 690 WP_137166112.1 YggS family pyridoxal phosphate-dependent enzyme -
  EZV72_RS04520 pilT 1062373..1063410 (+) 1038 WP_137166113.1 type IV pilus twitching motility protein PilT Machinery gene
  EZV72_RS04525 pilU 1063422..1064528 (+) 1107 WP_137166114.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EZV72_RS04530 - 1064531..1064899 (+) 369 WP_137166115.1 DUF2007 domain-containing protein -
  EZV72_RS04535 ruvX 1064896..1065324 (-) 429 WP_137166116.1 Holliday junction resolvase RuvX -
  EZV72_RS04540 - 1065317..1065883 (-) 567 WP_137166117.1 YqgE/AlgH family protein -
  EZV72_RS04545 gshB 1065888..1066847 (-) 960 WP_137166118.1 glutathione synthase -
  EZV72_RS04550 - 1066960..1067688 (-) 729 WP_137166119.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EZV72_RS04555 - 1068373..1069233 (+) 861 WP_137166120.1 mechanosensitive ion channel family protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40549.57 Da        Isoelectric Point: 5.7259

>NTDB_id=349512 EZV72_RS04525 WP_137166114.1 1063422..1064528(+) (pilU) [Salinimonas lutimaris strain DPSR-4]
MLIDYLRQATEQGASDIFVTAGFPVSAKINGELVAIADAPLSQFQAKELVHSIMEPRYIEELNVQKEANFAVGISGLGRF
RCSAFWQRDSVGMVIRRIVTEIPKADDLGLPPILKDVIMSKRGLVLMVGATGTGKSTSLAALINHRNHNSKGHILTIEDP
IEFIHRHGQCVVTQREVGIDTHSFDEALKSSLRQAPDVILLGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPRELHNKLLFDLSLNLRAIVAQQLVPRNDGGRVAAIEVLLNTPFVSELIQNNRIGELKEAMAKSKEAGMQTFDMALY
ELLNADIINMEQALHHADSPNDLRLMVKLDTNEGSNLGSLSNVSIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=349512 EZV72_RS04525 WP_137166114.1 1063422..1064528(+) (pilU) [Salinimonas lutimaris strain DPSR-4]
ATGTTAATTGATTATTTGCGCCAGGCCACCGAACAGGGCGCCTCGGATATTTTTGTGACAGCCGGTTTTCCGGTCAGCGC
TAAAATCAATGGCGAACTGGTGGCGATAGCGGATGCCCCGTTGTCTCAGTTTCAGGCCAAGGAGCTGGTGCACAGCATCA
TGGAGCCGCGGTACATTGAAGAGCTGAATGTTCAAAAAGAAGCAAACTTTGCTGTGGGTATCAGCGGACTGGGGCGGTTT
CGGTGCAGTGCATTCTGGCAGCGCGATTCTGTTGGCATGGTGATTCGCCGGATTGTCACCGAAATTCCTAAAGCCGACGA
TCTGGGGCTGCCGCCTATTTTAAAAGATGTAATCATGAGCAAGCGCGGGCTGGTTCTGATGGTAGGGGCGACCGGCACGG
GTAAGTCGACCTCGCTGGCGGCGCTGATAAATCACCGTAATCACAATTCAAAAGGCCATATACTGACCATTGAGGACCCG
ATTGAATTTATCCACCGCCACGGTCAGTGTGTAGTGACCCAGCGTGAAGTGGGCATTGATACTCATTCATTTGACGAAGC
ACTGAAAAGCTCTTTGCGCCAGGCCCCGGATGTGATTTTGCTTGGTGAGATTCGCAGCATGGAAACCATGGAGTATGCCA
TGTCTTTTGCTGACACCGGGCATTTGTGTGTTGCCACTTTGCATGCCAACAATGCCAATCAGGCTATTGAGCGGATCATG
CACCTGGCCCCCCGTGAGCTGCACAACAAGCTGCTGTTTGATTTGAGTCTGAATCTGCGTGCTATTGTGGCTCAGCAACT
GGTGCCACGTAATGATGGTGGACGGGTGGCGGCCATTGAAGTGCTGCTCAATACCCCGTTTGTCAGCGAATTGATACAAA
ATAACCGGATTGGTGAGCTTAAAGAAGCGATGGCAAAAAGCAAAGAGGCTGGTATGCAGACCTTCGATATGGCACTGTAT
GAGCTGCTCAATGCAGATATTATCAATATGGAGCAGGCCCTGCATCATGCTGACTCTCCAAATGATCTTCGTCTGATGGT
AAAACTGGATACCAACGAAGGCTCTAACCTGGGTTCACTGAGTAATGTATCCATTGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.821

97.283

0.562
  pilU Vibrio cholerae strain A1552

55.068

99.185

0.546
  pilU Acinetobacter baylyi ADP1

56.609

94.565

0.535
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.551

98.098

0.408
  pilT Legionella pneumophila strain Lp02

42.462

88.315

0.375
  pilT Legionella pneumophila strain ERS1305867

42.462

88.315

0.375

Multiple sequence alignment