Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EZZ25_RS02000 Genome accession   NZ_CP036499
Coordinates   427160..428197 (-) Length   345 a.a.
NCBI ID   WP_000350195.1    Uniprot ID   Q9KUQ3
Organism   Vibrio cholerae strain 20000     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422160..433197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZZ25_RS01975 (EZZ25_01975) - 422656..423219 (+) 564 WP_088124622.1 YqgE/AlgH family protein -
  EZZ25_RS01980 (EZZ25_01980) ruvX 423288..423710 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  EZZ25_RS01985 (EZZ25_01985) tyrS 423735..425015 (-) 1281 WP_144390760.1 tyrosine--tRNA ligase -
  EZZ25_RS01990 (EZZ25_01990) - 425124..425921 (-) 798 WP_155648880.1 helix-turn-helix transcriptional regulator -
  EZZ25_RS01995 (EZZ25_01995) pilU 426039..427145 (-) 1107 WP_000422572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EZZ25_RS02000 (EZZ25_02000) pilT 427160..428197 (-) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  EZZ25_RS02005 (EZZ25_02005) - 428223..428933 (+) 711 WP_001256648.1 YggS family pyridoxal phosphate-dependent enzyme -
  EZZ25_RS02010 (EZZ25_02010) proC 428986..429804 (+) 819 WP_000437946.1 pyrroline-5-carboxylate reductase -
  EZZ25_RS02015 (EZZ25_02015) - 429858..430415 (+) 558 WP_001087260.1 YggT family protein -
  EZZ25_RS02020 (EZZ25_02020) yggU 430415..430705 (+) 291 WP_000718490.1 DUF167 family protein YggU -
  EZZ25_RS02025 (EZZ25_02025) - 430751..431182 (+) 432 WP_001233674.1 DUF4426 domain-containing protein -
  EZZ25_RS02030 (EZZ25_02030) - 431252..431854 (+) 603 WP_000725016.1 XTP/dITP diphosphatase -
  EZZ25_RS02035 (EZZ25_02035) hemW 431848..433023 (+) 1176 WP_088124625.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38231.83 Da        Isoelectric Point: 6.6672

>NTDB_id=349214 EZZ25_RS02000 WP_000350195.1 427160..428197(-) (pilT) [Vibrio cholerae strain 20000]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=349214 EZZ25_RS02000 WP_000350195.1 427160..428197(-) (pilT) [Vibrio cholerae strain 20000]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTGCCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTTATCCCGACGTTAGA
GCAACTGGATGCTCCTGAGATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGGT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTGAACTATGTCAATGCTCATCACAATAAGCATATTTTGACTATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGTTCGGCATTGCGTGAAGACCCAGATGTGATCCTCGTCGGTGAGCTGCGTGACCAAGAAACCATTAGCTTGG
CGCTTACCGCGGCAGAAACCGGTCACTTGGTGTTTGGCACTCTACACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGCTGTCGGAATCGCTACGTGCGGTTATTGCGCAAAA
ACTCCTAAAACGCGTCGGGGGTGGCCGTGTCGCGTGTCATGAAATCATGCTGGCGACGCCGGCAATTCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATCGCTCGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCGTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KUQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

100

100

1
  pilT Vibrio cholerae strain A1552

100

100

1
  pilT Acinetobacter baumannii D1279779

71.726

97.391

0.699
  pilT Acinetobacter baumannii strain A118

71.726

97.391

0.699
  pilT Acinetobacter nosocomialis M2

71.429

97.391

0.696
  pilT Acinetobacter baylyi ADP1

72.424

95.652

0.693
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

64.545

95.652

0.617
  pilT Neisseria gonorrhoeae MS11

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.988

94.783

0.493
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.971

0.4
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment