Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EZZ25_RS01995 Genome accession   NZ_CP036499
Coordinates   426039..427145 (-) Length   368 a.a.
NCBI ID   WP_000422572.1    Uniprot ID   -
Organism   Vibrio cholerae strain 20000     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 421039..432145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZZ25_RS01970 (EZZ25_01970) gshB 421664..422620 (+) 957 WP_088124621.1 glutathione synthase -
  EZZ25_RS01975 (EZZ25_01975) - 422656..423219 (+) 564 WP_088124622.1 YqgE/AlgH family protein -
  EZZ25_RS01980 (EZZ25_01980) ruvX 423288..423710 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  EZZ25_RS01985 (EZZ25_01985) tyrS 423735..425015 (-) 1281 WP_144390760.1 tyrosine--tRNA ligase -
  EZZ25_RS01990 (EZZ25_01990) - 425124..425921 (-) 798 WP_155648880.1 helix-turn-helix transcriptional regulator -
  EZZ25_RS01995 (EZZ25_01995) pilU 426039..427145 (-) 1107 WP_000422572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EZZ25_RS02000 (EZZ25_02000) pilT 427160..428197 (-) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  EZZ25_RS02005 (EZZ25_02005) - 428223..428933 (+) 711 WP_001256648.1 YggS family pyridoxal phosphate-dependent enzyme -
  EZZ25_RS02010 (EZZ25_02010) proC 428986..429804 (+) 819 WP_000437946.1 pyrroline-5-carboxylate reductase -
  EZZ25_RS02015 (EZZ25_02015) - 429858..430415 (+) 558 WP_001087260.1 YggT family protein -
  EZZ25_RS02020 (EZZ25_02020) yggU 430415..430705 (+) 291 WP_000718490.1 DUF167 family protein YggU -
  EZZ25_RS02025 (EZZ25_02025) - 430751..431182 (+) 432 WP_001233674.1 DUF4426 domain-containing protein -
  EZZ25_RS02030 (EZZ25_02030) - 431252..431854 (+) 603 WP_000725016.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41307.27 Da        Isoelectric Point: 6.5076

>NTDB_id=349213 EZZ25_RS01995 WP_000422572.1 426039..427145(-) (pilU) [Vibrio cholerae strain 20000]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=349213 EZZ25_RS01995 WP_000422572.1 426039..427145(-) (pilU) [Vibrio cholerae strain 20000]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCACAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAGGCGAATTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTCAGTGCGTTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
GAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTCGGTGCAACCGGTTCGGGGA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCGATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAGGTGGGGCTCGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGTGAAATCCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACCGGGCATTTGTGTATGGCCACGCTGCACGCCAATAACGCCAACCAAGCGTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GCGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACTTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

100

100

1
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Vibrio cholerae strain A1552

42.216

90.761

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

90.761

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

42.462

88.315

0.375
  pilT Neisseria gonorrhoeae MS11

38.329

94.293

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361

Multiple sequence alignment