Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MSIP34_RS03160 Genome accession   NC_012969
Coordinates   645963..647006 (+) Length   347 a.a.
NCBI ID   WP_015829490.1    Uniprot ID   C6X9Q1
Organism   Methylovorus glucosotrophus SIP3-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 640963..652006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MSIP34_RS03130 (Msip34_0617) - 641002..641550 (+) 549 WP_015829485.1 DJ-1 family glyoxalase III -
  MSIP34_RS03135 (Msip34_0618) - 641547..642137 (+) 591 WP_015829486.1 nucleoside triphosphate pyrophosphatase -
  MSIP34_RS03140 (Msip34_0619) rng 642169..643626 (+) 1458 WP_013441450.1 ribonuclease G -
  MSIP34_RS03145 (Msip34_0620) - 643752..644327 (-) 576 WP_015829487.1 YggT family protein -
  MSIP34_RS03150 (Msip34_0621) proC 644329..645141 (-) 813 WP_015829488.1 pyrroline-5-carboxylate reductase -
  MSIP34_RS03155 (Msip34_0622) - 645219..645920 (-) 702 WP_015829489.1 YggS family pyridoxal phosphate-dependent enzyme -
  MSIP34_RS03160 (Msip34_0623) pilT 645963..647006 (+) 1044 WP_015829490.1 type IV pilus twitching motility protein PilT Machinery gene
  MSIP34_RS03165 (Msip34_0624) pilU 647055..648191 (+) 1137 WP_015829491.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MSIP34_RS03170 (Msip34_0625) - 648267..649235 (+) 969 WP_015829492.1 DUF72 domain-containing protein -
  MSIP34_RS03175 (Msip34_0626) - 649295..650572 (-) 1278 WP_015829493.1 dihydroorotase -
  MSIP34_RS03180 (Msip34_0627) - 650569..651522 (-) 954 WP_013441458.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38326.92 Da        Isoelectric Point: 6.5020

>NTDB_id=34791 MSIP34_RS03160 WP_015829490.1 645963..647006(+) (pilT) [Methylovorus glucosotrophus SIP3-4]
MDISDLLAFSVKNKASDLHLSAGMPPMIRVHGDVRKINLPSMDHSQVHDMVYDIMNDGQRKIYEETLECDFSFEIPNLAR
FRVNAFNQNRGAGAVFRTIPSKILSMEELNCPPIFREIAQNPRGIVLVTGPTGSGKSTTLAAMVDFINENEYGHILTVED
PIEFVHTSKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLCKAKDEQGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQNVGMQTLDQN
LQELVKRNIISVGEARGKAANKESFGG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=34791 MSIP34_RS03160 WP_015829490.1 645963..647006(+) (pilT) [Methylovorus glucosotrophus SIP3-4]
GTGGATATTTCGGATTTGCTGGCGTTCTCGGTTAAAAACAAGGCTTCTGATTTGCATCTTTCCGCCGGTATGCCACCCAT
GATCCGGGTGCATGGCGACGTGCGCAAAATCAACCTGCCCTCCATGGATCACAGCCAGGTGCACGACATGGTGTATGACA
TCATGAACGACGGCCAGCGCAAGATTTACGAAGAAACCCTGGAGTGCGATTTCTCGTTCGAGATTCCCAATCTTGCCCGT
TTCCGGGTCAATGCCTTCAACCAGAATCGCGGTGCGGGCGCGGTATTTCGTACCATTCCTTCCAAGATTCTGAGCATGGA
AGAGCTGAACTGTCCGCCTATCTTCCGTGAAATCGCGCAAAACCCGCGCGGCATCGTGCTGGTGACTGGCCCTACCGGTT
CCGGCAAATCCACCACGCTGGCGGCCATGGTCGATTTCATCAATGAAAACGAGTACGGCCATATCCTGACGGTGGAAGAC
CCGATTGAGTTCGTCCACACCTCGAAAAAGTGTTTGATCAACCAGCGCGAAGTCGGTCCGCATACCCTGTCGTTCTCGAA
TGCCTTGCGCTCAGCGCTGCGTGAAGACCCTGATGTGATTCTGGTTGGCGAAATGCGCGACCTGGAAACCATACGCCTGG
CCTTGTCGGCCGCTGAAACCGGCCACCTGGTGTTTGGCACCCTACATACCAGCTCGGCGGCCAAGACCATAGACCGTATC
GTCGATGTATTCCCCGCTGCCGAAAAGGAAATGGTGCGCTCCATGCTCTCCGAGTCGTTGCGGGCGGTGATCTCGCAAAC
CCTGTGCAAAGCCAAGGATGAACAGGGCCGTGTTGCCGCGCATGAGATCATGATAGGCACGCCGGCCATCCGTAACCTCA
TCCGCGAGAACAAGATTGCGCAGATGTATTCCGCCATCCAGACCGGGCAGAATGTGGGCATGCAGACGCTGGACCAGAAC
CTGCAGGAGCTGGTGAAGCGCAATATCATTTCGGTGGGCGAAGCCCGAGGCAAGGCCGCCAACAAGGAATCCTTCGGCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6X9Q1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.681

99.424

0.772
  pilT Acinetobacter baumannii D1279779

77.681

99.424

0.772
  pilT Acinetobacter baumannii strain A118

77.681

99.424

0.772
  pilT Acinetobacter baylyi ADP1

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Pseudomonas aeruginosa PAK

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

74.783

99.424

0.744
  pilT Legionella pneumophila strain ERS1305867

74.783

99.424

0.744
  pilT Neisseria meningitidis 8013

71.884

99.424

0.715
  pilT Neisseria gonorrhoeae MS11

71.594

99.424

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.751

97.406

0.66
  pilT Vibrio cholerae strain A1552

67.751

97.406

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Acinetobacter baylyi ADP1

42.56

96.83

0.412
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.542

0.392
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392

Multiple sequence alignment