Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MSIP34_RS03165 Genome accession   NC_012969
Coordinates   647055..648191 (+) Length   378 a.a.
NCBI ID   WP_015829491.1    Uniprot ID   C6X9Q2
Organism   Methylovorus glucosotrophus SIP3-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 642055..653191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MSIP34_RS03140 (Msip34_0619) rng 642169..643626 (+) 1458 WP_013441450.1 ribonuclease G -
  MSIP34_RS03145 (Msip34_0620) - 643752..644327 (-) 576 WP_015829487.1 YggT family protein -
  MSIP34_RS03150 (Msip34_0621) proC 644329..645141 (-) 813 WP_015829488.1 pyrroline-5-carboxylate reductase -
  MSIP34_RS03155 (Msip34_0622) - 645219..645920 (-) 702 WP_015829489.1 YggS family pyridoxal phosphate-dependent enzyme -
  MSIP34_RS03160 (Msip34_0623) pilT 645963..647006 (+) 1044 WP_015829490.1 type IV pilus twitching motility protein PilT Machinery gene
  MSIP34_RS03165 (Msip34_0624) pilU 647055..648191 (+) 1137 WP_015829491.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MSIP34_RS03170 (Msip34_0625) - 648267..649235 (+) 969 WP_015829492.1 DUF72 domain-containing protein -
  MSIP34_RS03175 (Msip34_0626) - 649295..650572 (-) 1278 WP_015829493.1 dihydroorotase -
  MSIP34_RS03180 (Msip34_0627) - 650569..651522 (-) 954 WP_013441458.1 aspartate carbamoyltransferase catalytic subunit -
  MSIP34_RS03185 (Msip34_0628) pyrR 651522..652019 (-) 498 WP_015829494.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  MSIP34_RS03190 (Msip34_0629) ruvX 652043..652561 (-) 519 WP_015829495.1 Holliday junction resolvase RuvX -
  MSIP34_RS03195 (Msip34_0630) - 652594..653154 (-) 561 WP_013441461.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42504.05 Da        Isoelectric Point: 6.1869

>NTDB_id=34792 MSIP34_RS03165 WP_015829491.1 647055..648191(+) (pilU) [Methylovorus glucosotrophus SIP3-4]
MEEGQAEKFVFDLLRMMIAKKASDLFITAAFPPAMKIDGKMTPMSSQPLTPQHVREIARAIMNDKQAAEFEATRECNFAI
SMPRVARFRVNAFVQRGSVGLVFRTITSAIPKFDDLRLPEVLKEVSMTKRGLVILVGGTGSGKSTTLAAMMGYRNENSYG
HIITIEDPIEFIHDHKNCIVTQREVGVDTDNWFAALKNTLRQAPDVIMIGEIRDRETMDYAIAFAETGHLCMATLHANST
NQALDRIINFFPEERRQQLLMDLSLNMRAFVSQRLIPKRDGQGRAAAIEIMLNSPLISDLVLKGEVHGIKEIMAKSRELG
MQTFDQALFDLYERDEISYEDALRNADSINDIRLKIKLEGKDAQDRDMSSGLDKLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=34792 MSIP34_RS03165 WP_015829491.1 647055..648191(+) (pilU) [Methylovorus glucosotrophus SIP3-4]
ATGGAAGAAGGTCAGGCAGAGAAGTTTGTTTTTGATTTGTTGCGCATGATGATTGCCAAGAAAGCCTCTGATCTTTTCAT
CACGGCGGCCTTTCCACCGGCAATGAAGATCGATGGCAAGATGACGCCCATGAGCAGCCAGCCACTCACGCCTCAGCACG
TGCGCGAGATTGCCCGCGCCATCATGAATGACAAGCAGGCTGCCGAATTTGAAGCGACCCGCGAATGCAATTTCGCCATC
AGCATGCCGCGTGTGGCCCGGTTCCGGGTCAATGCCTTTGTGCAGCGTGGCTCGGTCGGTCTGGTATTCCGGACCATTAC
CTCGGCTATTCCCAAGTTTGATGACCTGCGCCTGCCCGAGGTGTTGAAGGAAGTCTCCATGACCAAGCGTGGCCTGGTGA
TTCTGGTCGGCGGCACGGGCTCCGGCAAATCCACCACGCTGGCGGCGATGATGGGCTACCGCAACGAAAACAGTTACGGT
CATATCATCACCATTGAAGACCCGATTGAATTCATACATGACCACAAAAACTGCATCGTGACCCAGCGCGAAGTGGGGGT
GGATACCGATAACTGGTTTGCCGCGCTGAAAAACACCCTGCGCCAGGCGCCTGATGTGATCATGATCGGCGAAATCCGCG
ATCGCGAAACCATGGACTACGCCATTGCCTTTGCCGAAACCGGCCACCTGTGCATGGCAACACTGCACGCCAACAGCACC
AACCAGGCGCTGGACCGTATCATCAACTTCTTCCCTGAAGAGCGTCGTCAGCAATTGCTGATGGATCTGTCGCTGAACAT
GCGGGCCTTTGTCTCCCAGCGTCTGATCCCCAAGCGCGATGGTCAGGGCCGTGCGGCGGCGATTGAGATCATGCTGAATT
CCCCCCTTATTTCTGACCTGGTGCTGAAGGGCGAAGTGCATGGCATCAAGGAGATCATGGCGAAGTCACGCGAACTGGGC
ATGCAGACCTTTGACCAGGCCCTGTTTGATTTGTATGAGCGCGATGAAATCAGCTACGAAGATGCGCTGCGCAATGCAGA
CTCCATCAATGATATCCGCCTCAAGATCAAGCTGGAAGGCAAGGACGCGCAAGACCGCGATATGTCCTCCGGGCTCGATA
AACTGGGAATAGTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6X9Q2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.732

94.709

0.585
  pilU Acinetobacter baylyi ADP1

60.345

92.063

0.556
  pilU Vibrio cholerae strain A1552

54.648

93.915

0.513
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.323

89.153

0.386
  pilT Legionella pneumophila strain Lp02

43.544

88.095

0.384
  pilT Legionella pneumophila strain ERS1305867

43.544

88.095

0.384
  pilT Acinetobacter nosocomialis M2

42.943

88.095

0.378
  pilT Acinetobacter baumannii D1279779

42.943

88.095

0.378
  pilT Acinetobacter baumannii strain A118

42.943

88.095

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.433

89.153

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.462

85.979

0.365
  pilT Vibrio cholerae strain A1552

42.462

85.979

0.365
  pilT Acinetobacter baylyi ADP1

41.141

88.095

0.362

Multiple sequence alignment