Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EYC57_RS06640 Genome accession   NZ_CP036256
Coordinates   1395850..1396887 (+) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas oryzae strain BAI23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1390850..1401887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EYC57_RS06610 (EYC57_06610) - 1391178..1391720 (-) 543 WP_131074823.1 hypothetical protein -
  EYC57_RS06615 (EYC57_06615) - 1391778..1392119 (-) 342 WP_131074824.1 DUF3861 family protein -
  EYC57_RS06620 (EYC57_06620) - 1392177..1392818 (-) 642 WP_131074825.1 M23 family metallopeptidase -
  EYC57_RS06625 (EYC57_06625) - 1392924..1393349 (-) 426 WP_011258216.1 HU family DNA-binding protein -
  EYC57_RS06630 (EYC57_06630) proC 1393906..1394766 (-) 861 WP_131074826.1 pyrroline-5-carboxylate reductase -
  EYC57_RS06635 (EYC57_06635) - 1394810..1395502 (-) 693 WP_131074827.1 YggS family pyridoxal phosphate-dependent enzyme -
  EYC57_RS06640 (EYC57_06640) pilT 1395850..1396887 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  EYC57_RS06645 (EYC57_06645) pilU 1397001..1398131 (+) 1131 WP_131074828.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EYC57_RS06650 (EYC57_06650) - 1398403..1399077 (+) 675 WP_024711780.1 YitT family protein -
  EYC57_RS06660 (EYC57_06660) - 1399304..1400137 (-) 834 WP_131074829.1 DUF72 domain-containing protein -
  EYC57_RS06665 (EYC57_06665) - 1400174..1400746 (-) 573 WP_014502539.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=347727 EYC57_RS06640 WP_003489919.1 1395850..1396887(+) (pilT) [Xanthomonas oryzae strain BAI23]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=347727 EYC57_RS06640 WP_003489919.1 1395850..1396887(+) (pilT) [Xanthomonas oryzae strain BAI23]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCGCTGGACCATAAGCAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTACGAGGAATTCCTCGAAGTCGACTTCTCGTTCGAAATTCCGTCGCTGGCGCGT
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCCTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCTTGATCCTGGTGACCGGCCCGACTGGTT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAATCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGTTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCAGTGATTTCGCAGGC
GCTGCTGAAGAAAGTCGGCGGCGGGCGTACCGCAGCATGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTCGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCGCGCGAGTACGCCAAGGACAAGCGGATATTCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment