Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EYC57_RS06645 Genome accession   NZ_CP036256
Coordinates   1397001..1398131 (+) Length   376 a.a.
NCBI ID   WP_131074828.1    Uniprot ID   -
Organism   Xanthomonas oryzae strain BAI23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1392001..1403131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EYC57_RS06620 (EYC57_06620) - 1392177..1392818 (-) 642 WP_131074825.1 M23 family metallopeptidase -
  EYC57_RS06625 (EYC57_06625) - 1392924..1393349 (-) 426 WP_011258216.1 HU family DNA-binding protein -
  EYC57_RS06630 (EYC57_06630) proC 1393906..1394766 (-) 861 WP_131074826.1 pyrroline-5-carboxylate reductase -
  EYC57_RS06635 (EYC57_06635) - 1394810..1395502 (-) 693 WP_131074827.1 YggS family pyridoxal phosphate-dependent enzyme -
  EYC57_RS06640 (EYC57_06640) pilT 1395850..1396887 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  EYC57_RS06645 (EYC57_06645) pilU 1397001..1398131 (+) 1131 WP_131074828.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EYC57_RS06650 (EYC57_06650) - 1398403..1399077 (+) 675 WP_024711780.1 YitT family protein -
  EYC57_RS06660 (EYC57_06660) - 1399304..1400137 (-) 834 WP_131074829.1 DUF72 domain-containing protein -
  EYC57_RS06665 (EYC57_06665) - 1400174..1400746 (-) 573 WP_014502539.1 DNA-3-methyladenine glycosylase I -
  EYC57_RS23780 - 1400743..1401953 (-) 1211 Protein_1214 hypothetical protein -
  EYC57_RS06680 (EYC57_06680) - 1402029..1402595 (+) 567 WP_053503124.1 YqgE/AlgH family protein -
  EYC57_RS06685 (EYC57_06685) ruvX 1402588..1403055 (+) 468 WP_014502544.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41956.17 Da        Isoelectric Point: 6.8286

>NTDB_id=347728 EYC57_RS06645 WP_131074828.1 1397001..1398131(+) (pilU) [Xanthomonas oryzae strain BAI23]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGHRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWESALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKDIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGMDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=347728 EYC57_RS06645 WP_131074828.1 1397001..1398131(+) (pilU) [Xanthomonas oryzae strain BAI23]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCACCGGCGATCAAGGTGAATGGCAAGATCAGCCCGATTACCCAGACGCCGCTGACTGCGCAGCAGAGCCGCGATCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTTTCAGGG
GTGGGGCGTTTTCGTGTGAGCTGTTTCTACCAGCGTAATCAGGTCGGTATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCCTGCCTCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCCACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGAAAGCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAATCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCAATCAGCTGCTGATGGACCTGTCGCTCAATCTCAAGGGCGTGGT
GGCGCAGCAGCTGATTCCGACGCCGGATGGCCGCAGCCGCCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAAATCCACAAGCTCAAGGACATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCTGGCGAAATCAGCTACGAAGATGCATTGCGCTACGCCGACTCGCAGAACGA
AGTGCGCTTGCGCATCAAGCTCTCGCAGGGGGGCGATGCCAAGACCTTGTCGCAGGGCATGGATGGTGTGGAGATTTCCG
AAGTTCGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.706

94.947

0.614
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter nosocomialis M2

39.535

91.489

0.362
  pilT Acinetobacter baumannii D1279779

39.535

91.489

0.362
  pilT Acinetobacter baumannii strain A118

39.535

91.489

0.362

Multiple sequence alignment