Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MMOL_RS03200 Genome accession   NC_012968
Coordinates   668137..669180 (+) Length   347 a.a.
NCBI ID   WP_015831579.1    Uniprot ID   C6WUE3
Organism   Methylotenera mobilis JLW8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 663137..674180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMOL_RS03180 (Mmol_0628) - 663724..665811 (-) 2088 WP_015831575.1 dynamin family protein -
  MMOL_RS03185 (Mmol_0629) - 665826..666401 (-) 576 WP_015831576.1 YggT family protein -
  MMOL_RS03190 (Mmol_0630) proC 666418..667224 (-) 807 WP_015831577.1 pyrroline-5-carboxylate reductase -
  MMOL_RS03195 (Mmol_0631) - 667365..668063 (-) 699 WP_015831578.1 YggS family pyridoxal phosphate-dependent enzyme -
  MMOL_RS03200 (Mmol_0632) pilT 668137..669180 (+) 1044 WP_015831579.1 type IV pilus twitching motility protein PilT Machinery gene
  MMOL_RS03205 (Mmol_0633) pilU 669318..670454 (+) 1137 WP_015831580.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MMOL_RS03210 (Mmol_0634) - 670556..671830 (-) 1275 WP_015831581.1 dihydroorotase -
  MMOL_RS03215 (Mmol_0635) - 671827..672789 (-) 963 WP_015831582.1 aspartate carbamoyltransferase catalytic subunit -
  MMOL_RS03220 (Mmol_0636) pyrR 672794..673351 (-) 558 WP_015831583.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  MMOL_RS03225 (Mmol_0637) ruvX 673437..673907 (-) 471 WP_015831584.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38311.97 Da        Isoelectric Point: 6.5735

>NTDB_id=34765 MMOL_RS03200 WP_015831579.1 668137..669180(+) (pilT) [Methylotenera mobilis JLW8]
MDISDLLAFSVKNKASDLHLSAGLPPMIRVHGDIRKINVPAMDHSEVHDMVYDIMNDGQRKVYEETLECDFSFEIPNLAR
FRVNAFNHNRGSGAVFRTIPSKILTLEQLNCPAIFKDIADTPRGICLVTGPTGSGKSTTLAAMIDYINDKEFGHILTVED
PIEFVHQSKKCLINQREVGPHTLSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHMVFGTLHTSSAAKTVDRI
IDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDEQGRVAAHEIMIGTPAIRNLIRENKIPQMYSAIQTGQNVGMQTLDQN
LQDLVKRGVVSANEARAKAANKDAIMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=34765 MMOL_RS03200 WP_015831579.1 668137..669180(+) (pilT) [Methylotenera mobilis JLW8]
GTGGATATTTCGGATTTACTCGCATTTTCAGTGAAAAATAAAGCCTCAGATTTACACTTATCTGCCGGCCTGCCTCCGAT
GATCCGTGTACACGGGGATATTAGAAAAATTAATGTACCGGCGATGGATCACAGCGAAGTGCATGACATGGTGTATGACA
TCATGAATGATGGGCAGCGCAAGGTGTATGAAGAAACGCTAGAGTGTGACTTTTCATTCGAGATTCCCAACTTGGCGCGT
TTTCGTGTGAATGCTTTTAACCATAATCGTGGCTCTGGCGCTGTATTCCGTACCATTCCTTCAAAAATACTAACCTTAGA
GCAGCTAAATTGCCCGGCTATTTTTAAAGATATTGCCGATACCCCGCGTGGTATCTGTTTGGTAACAGGGCCGACTGGTT
CCGGTAAATCCACCACGTTGGCGGCGATGATTGACTATATTAACGATAAAGAATTTGGGCATATCCTGACGGTAGAAGAC
CCGATTGAGTTTGTGCATCAATCTAAAAAATGTTTAATTAACCAACGTGAAGTTGGTCCGCATACCTTGTCATTTAACAA
TGCATTACGTTCAGCATTGCGTGAGGACCCGGACGTTATCTTGGTGGGTGAGTTGCGTGATCTGGAGACTATCCGCTTAG
CGCTTACGGCCGCGGAAACCGGGCATATGGTGTTTGGTACCTTACATACAAGCTCTGCTGCTAAAACGGTAGACCGTATT
ATTGACGTGTTCCCAGCAGCAGAAAAAGAAATGGTGCGCTCTATGCTGTCTGAATCTTTACGCGCGGTGATTTCGCAAAC
CTTGTTAAAAACTAAAGATGAGCAGGGGCGTGTTGCTGCGCACGAGATTATGATCGGGACGCCGGCGATTCGTAACCTGA
TTCGTGAAAATAAAATTCCGCAAATGTATTCTGCGATTCAAACTGGGCAAAATGTCGGTATGCAGACCTTGGATCAGAAC
TTACAGGATTTAGTAAAGCGCGGTGTGGTTTCGGCCAACGAAGCACGTGCAAAAGCAGCAAACAAAGATGCGATTATGGG
TTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6WUE3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.012

99.712

0.758
  pilT Acinetobacter baumannii D1279779

76.012

99.712

0.758
  pilT Acinetobacter baumannii strain A118

76.012

99.712

0.758
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

72.807

98.559

0.718
  pilT Legionella pneumophila strain ERS1305867

72.807

98.559

0.718
  pilT Pseudomonas stutzeri DSM 10701

73.451

97.695

0.718
  pilT Pseudomonas aeruginosa PAK

73.156

97.695

0.715
  pilT Neisseria meningitidis 8013

71.304

99.424

0.709
  pilT Neisseria gonorrhoeae MS11

71.014

99.424

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.822

97.406

0.68
  pilT Vibrio cholerae strain A1552

69.822

97.406

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

97.695

0.493
  pilU Acinetobacter baylyi ADP1

42.353

97.983

0.415
  pilU Vibrio cholerae strain A1552

40.23

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403

Multiple sequence alignment