Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MMOL_RS03205 Genome accession   NC_012968
Coordinates   669318..670454 (+) Length   378 a.a.
NCBI ID   WP_015831580.1    Uniprot ID   C6WUE4
Organism   Methylotenera mobilis JLW8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 664318..675454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMOL_RS03185 (Mmol_0629) - 665826..666401 (-) 576 WP_015831576.1 YggT family protein -
  MMOL_RS03190 (Mmol_0630) proC 666418..667224 (-) 807 WP_015831577.1 pyrroline-5-carboxylate reductase -
  MMOL_RS03195 (Mmol_0631) - 667365..668063 (-) 699 WP_015831578.1 YggS family pyridoxal phosphate-dependent enzyme -
  MMOL_RS03200 (Mmol_0632) pilT 668137..669180 (+) 1044 WP_015831579.1 type IV pilus twitching motility protein PilT Machinery gene
  MMOL_RS03205 (Mmol_0633) pilU 669318..670454 (+) 1137 WP_015831580.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MMOL_RS03210 (Mmol_0634) - 670556..671830 (-) 1275 WP_015831581.1 dihydroorotase -
  MMOL_RS03215 (Mmol_0635) - 671827..672789 (-) 963 WP_015831582.1 aspartate carbamoyltransferase catalytic subunit -
  MMOL_RS03220 (Mmol_0636) pyrR 672794..673351 (-) 558 WP_015831583.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  MMOL_RS03225 (Mmol_0637) ruvX 673437..673907 (-) 471 WP_015831584.1 Holliday junction resolvase RuvX -
  MMOL_RS03230 (Mmol_0638) - 673925..674506 (-) 582 WP_015831585.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41867.22 Da        Isoelectric Point: 6.4204

>NTDB_id=34766 MMOL_RS03205 WP_015831580.1 669318..670454(+) (pilU) [Methylotenera mobilis JLW8]
MEKGQAEKFVFDLLRLMLAKNASDLFITVGFPPAIKVDGKVTPLSSQVLTALQAREISRAIMNDKQTAEFDATNECNFAI
GIPSVARFRVNAFVQRGTVGLVFRTITTKIPEIDELGLPPVLKDVAMTKRGLVIFVGGTGSGKSTSLAAMVGYRNQNSYG
HIITIEDPVEFVHEHKNCIITQREVGVDTDNWHVALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHANST
NQALDRIINFFPEERRHQLLMDLSLNVRAFVSQRLIPKKEGKGRTAAMEVMLNSPLISDLIFKGEVHEIKEIIAKSRELG
MQTFDQSLFDLYEADAITYEDALRNADSVNDLRLKIKLEGKGAHSKDLSAGLDALGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=34766 MMOL_RS03205 WP_015831580.1 669318..670454(+) (pilU) [Methylotenera mobilis JLW8]
ATGGAAAAAGGGCAGGCTGAGAAGTTTGTATTCGACTTATTACGGTTGATGTTGGCAAAAAATGCCTCTGATTTGTTCAT
TACCGTTGGCTTCCCGCCGGCAATCAAGGTGGATGGTAAGGTAACGCCACTCAGCAGTCAGGTATTAACAGCGCTGCAGG
CACGTGAAATCTCACGCGCGATTATGAACGACAAGCAAACCGCTGAATTTGATGCTACCAATGAGTGTAATTTTGCGATT
GGCATCCCAAGTGTTGCGCGCTTCCGCGTAAATGCTTTTGTACAGCGCGGTACTGTGGGCTTAGTGTTTAGAACGATTAC
CACCAAAATTCCTGAAATCGATGAATTGGGCTTGCCGCCAGTACTCAAAGACGTGGCAATGACTAAACGTGGATTGGTCA
TTTTTGTGGGTGGTACCGGTTCTGGTAAGTCTACCTCACTGGCGGCGATGGTGGGCTATCGTAACCAAAATAGCTACGGG
CATATTATTACCATTGAAGACCCGGTGGAGTTCGTGCATGAGCACAAGAACTGTATCATCACCCAGCGTGAGGTGGGGGT
GGACACGGACAATTGGCATGTTGCCCTCAAAAACACCTTACGTCAGGCGCCAGATGTGATTTTGATTGGTGAGATTCGCG
ATCGCGAAACCATGGACTATGCAATTGCGTTTGCCGAAACCGGCCACCTTTGCATGGCTACATTGCATGCGAATAGTACC
AATCAAGCATTGGATCGCATTATTAACTTCTTCCCGGAAGAGCGTCGCCATCAACTACTGATGGACTTATCACTCAACGT
GCGTGCATTTGTGTCACAGCGCTTGATTCCTAAAAAAGAAGGCAAGGGCAGAACTGCCGCCATGGAAGTCATGCTTAACT
CACCATTAATCTCCGATTTAATCTTCAAAGGCGAAGTGCATGAAATTAAAGAGATTATCGCTAAATCGCGCGAATTGGGG
ATGCAAACCTTTGACCAATCATTGTTTGATTTGTATGAGGCGGATGCCATTACTTACGAAGATGCCTTGCGCAATGCTGA
CTCGGTGAATGACCTGCGCCTCAAAATTAAACTGGAAGGTAAAGGTGCGCACAGCAAAGACCTATCCGCCGGACTTGATG
CATTGGGTATTGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6WUE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.707

95.767

0.601
  pilU Acinetobacter baylyi ADP1

60.92

92.063

0.561
  pilU Vibrio cholerae strain A1552

57.771

90.212

0.521
  pilT Pseudomonas aeruginosa PAK

44.807

89.153

0.399
  pilT Acinetobacter nosocomialis M2

44.345

88.889

0.394
  pilT Acinetobacter baumannii D1279779

44.345

88.889

0.394
  pilT Acinetobacter baumannii strain A118

44.345

88.889

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

89.153

0.392
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

44.72

85.185

0.381
  pilT Legionella pneumophila strain Lp02

42.643

88.095

0.376
  pilT Legionella pneumophila strain ERS1305867

42.643

88.095

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.711

84.127

0.368
  pilT Vibrio cholerae strain A1552

43.711

84.127

0.368

Multiple sequence alignment