Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EXU30_RS03665 Genome accession   NZ_CP036200
Coordinates   859531..860568 (-) Length   345 a.a.
NCBI ID   WP_130597870.1    Uniprot ID   -
Organism   Shewanella maritima strain D4-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 854531..865568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXU30_RS03640 (EXU30_03640) - 855097..855687 (-) 591 WP_130597865.1 YqgE/AlgH family protein -
  EXU30_RS03645 (EXU30_03645) yciH 855729..856058 (-) 330 WP_130597866.1 stress response translation initiation inhibitor YciH -
  EXU30_RS03650 (EXU30_03650) hemH 856218..857234 (-) 1017 WP_130597867.1 ferrochelatase -
  EXU30_RS03655 (EXU30_03655) - 857429..858010 (-) 582 WP_130597868.1 glutathione peroxidase -
  EXU30_RS03660 (EXU30_03660) pilU 858380..859495 (-) 1116 WP_130597869.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EXU30_RS03665 (EXU30_03665) pilT 859531..860568 (-) 1038 WP_130597870.1 type IV pilus twitching motility protein PilT Machinery gene
  EXU30_RS03670 (EXU30_03670) - 860596..861285 (+) 690 WP_130597871.1 YggS family pyridoxal phosphate-dependent enzyme -
  EXU30_RS03675 (EXU30_03675) proC 861383..862201 (+) 819 WP_130597872.1 pyrroline-5-carboxylate reductase -
  EXU30_RS03680 (EXU30_03680) - 862832..863380 (+) 549 WP_130597873.1 YggT family protein -
  EXU30_RS03685 (EXU30_03685) - 863583..864023 (+) 441 WP_130597874.1 DUF4426 domain-containing protein -
  EXU30_RS03690 (EXU30_03690) rdgB 864238..864834 (+) 597 WP_130597875.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38272.85 Da        Isoelectric Point: 6.9096

>NTDB_id=347496 EXU30_RS03665 WP_130597870.1 859531..860568(-) (pilT) [Shewanella maritima strain D4-2]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGEVRKINVPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPGLAR
FRVNAFNQSRGAGAVFRTIPSEILTLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHQNKQCLVNQREVHKHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGQITREDAMSKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=347496 EXU30_RS03665 WP_130597870.1 859531..860568(-) (pilT) [Shewanella maritima strain D4-2]
ATGGAAATCACTGAATTACTAGCCTTTAGTGTAAAACACAAGGCGTCAGATTTGCACCTTTCTGCAGGTGTTTCACCTAT
GATCCGTGTTGATGGTGAAGTGCGTAAAATTAACGTGCCAGCACTCGATCATCAAGGTGTTCATAGCTTAGTTTATGACA
TCATGAACGATAAGCAGCGTAAAGACTATGAAGAGCACCTTGAAATTGACTTTTCGTTTGAAGTACCTGGTCTTGCGCGT
TTTCGTGTAAACGCATTTAACCAGTCTCGCGGTGCTGGTGCTGTTTTCCGTACTATTCCCTCTGAAATCTTAACTCTTGA
GCAGCTAGGCGCGCCTGAAATCTTTAAAAAGATCTCTAGTTTCCCTCGTGGCCTAGTGCTGGTCACTGGCCCTACTGGTT
CGGGTAAGTCAACCACGCTTGCTGCTATGGTCGACTATATTAACGAGAATCGTCACGACCACATTTTGACCATTGAAGAC
CCTATTGAATTCGTGCACCAGAACAAACAATGTTTGGTTAACCAACGTGAGGTGCACAAACATACTCATAGCTTCAACGC
TGCGCTACGTAGTGCACTGCGTGAAGACCCTGACGTTATTCTTGTGGGTGAGATGCGTGACCTTGAAACCATTCGCCTAG
CAATGACGGCAGCAGAAACCGGTCACCTAGTGTTTGGTACCTTACATACCACCTCAGCGGCAAAAACCATTGACCGTGTG
GTTGACGTATTCCCAGCGGGTGAAAAAGACATGGTAAGAACCATGTTGTCAGAGTCACTACAGGCGGTTATTTCGCAAAC
TCTTATCAAGAAAATCGGTGGCGGGCGAGTCGCCGCTCACGAGATCATGATGGGTACGCCAGCTATTCGTAACCTCATTC
GTGAAGATAAAGTCGCGCAAATGTACTCGGCAATTCAAACTGGTATGGCTCATGGCATGCAGACGCTTGAGCAATGTTTG
CAAAACCTGGTTAACCGCGGCCAAATTACCCGTGAAGATGCGATGTCGAAGAGCTCGAATAAACAAGCTAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.3

99.42

0.788
  pilT Acinetobacter baumannii D1279779

79.3

99.42

0.788
  pilT Acinetobacter baumannii strain A118

79.3

99.42

0.788
  pilT Pseudomonas aeruginosa PAK

79.586

97.971

0.78
  pilT Pseudomonas stutzeri DSM 10701

77.391

100

0.774
  pilT Acinetobacter baylyi ADP1

79.939

95.362

0.762
  pilT Legionella pneumophila strain Lp02

74.78

98.841

0.739
  pilT Legionella pneumophila strain ERS1305867

74.78

98.841

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.681

0.71
  pilT Vibrio cholerae strain A1552

72.7

97.681

0.71
  pilT Neisseria meningitidis 8013

67.733

99.71

0.675
  pilT Neisseria gonorrhoeae MS11

67.442

99.71

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.558

98.261

0.487
  pilU Pseudomonas stutzeri DSM 10701

42.857

99.42

0.426
  pilU Vibrio cholerae strain A1552

41.964

97.391

0.409
  pilU Acinetobacter baylyi ADP1

39.466

97.681

0.386

Multiple sequence alignment