Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EXU30_RS03660 Genome accession   NZ_CP036200
Coordinates   858380..859495 (-) Length   371 a.a.
NCBI ID   WP_130597869.1    Uniprot ID   A0A411PED6
Organism   Shewanella maritima strain D4-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 853380..864495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXU30_RS03630 (EXU30_03630) - 854053..854319 (-) 267 WP_242620311.1 GIY-YIG nuclease family protein -
  EXU30_RS03635 (EXU30_03635) ruvX 854507..854929 (-) 423 WP_130597864.1 Holliday junction resolvase RuvX -
  EXU30_RS03640 (EXU30_03640) - 855097..855687 (-) 591 WP_130597865.1 YqgE/AlgH family protein -
  EXU30_RS03645 (EXU30_03645) yciH 855729..856058 (-) 330 WP_130597866.1 stress response translation initiation inhibitor YciH -
  EXU30_RS03650 (EXU30_03650) hemH 856218..857234 (-) 1017 WP_130597867.1 ferrochelatase -
  EXU30_RS03655 (EXU30_03655) - 857429..858010 (-) 582 WP_130597868.1 glutathione peroxidase -
  EXU30_RS03660 (EXU30_03660) pilU 858380..859495 (-) 1116 WP_130597869.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EXU30_RS03665 (EXU30_03665) pilT 859531..860568 (-) 1038 WP_130597870.1 type IV pilus twitching motility protein PilT Machinery gene
  EXU30_RS03670 (EXU30_03670) - 860596..861285 (+) 690 WP_130597871.1 YggS family pyridoxal phosphate-dependent enzyme -
  EXU30_RS03675 (EXU30_03675) proC 861383..862201 (+) 819 WP_130597872.1 pyrroline-5-carboxylate reductase -
  EXU30_RS03680 (EXU30_03680) - 862832..863380 (+) 549 WP_130597873.1 YggT family protein -
  EXU30_RS03685 (EXU30_03685) - 863583..864023 (+) 441 WP_130597874.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41089.93 Da        Isoelectric Point: 5.4302

>NTDB_id=347495 EXU30_RS03660 WP_130597869.1 858380..859495(-) (pilU) [Shewanella maritima strain D4-2]
MDVTPFLKVMVEKKASDLFITAGFPPSAKIDGELRALSEHSFNSEQSLDFVESLMTDAQKQEFKDTSECNFAFAAKELGR
FRVSAFWQRESAGCVMRRIETRIPEVEELKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPENKHNQLLFDLSLNLRGIVAQQLVAKSDGSGRRAAIEILINTPRVADLIAKNDLHLLKETMAKSNEQGMQTFDQA
LLALYLEGEISYADALHHADSPNDLRLMIKLQNNDTSSTGGFMEGVTLDLD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=347495 EXU30_RS03660 WP_130597869.1 858380..859495(-) (pilU) [Shewanella maritima strain D4-2]
ATGGATGTCACACCATTTCTAAAAGTAATGGTTGAGAAGAAAGCCTCGGATTTATTTATCACTGCAGGCTTTCCACCCAG
CGCCAAAATTGATGGTGAGCTACGTGCGTTAAGTGAGCATAGCTTTAACTCTGAGCAGTCATTAGATTTCGTAGAATCGC
TAATGACAGATGCGCAAAAACAAGAGTTTAAAGATACCAGCGAGTGTAACTTTGCATTTGCTGCTAAAGAACTTGGCCGT
TTTCGTGTTAGCGCATTTTGGCAGCGAGAATCTGCAGGTTGTGTGATGCGTCGTATCGAAACGCGCATTCCTGAAGTGGA
AGAACTAAAACTACCGCCAATTTTAAAAGACCTAGTTATGAGTAAACGTGGTCTTATTATTATGGTTGGTGGTACAGGTA
CGGGTAAGTCTACCTCGCTTGCAGCCTTGGTTGGTTATCGTAATGCTCACGCTCGCGGGCACATTTTAACCATTGAGGAC
CCGGTAGAATTTGTGCACGATCATCGTAAAAGCATTATTACTCAGCGCGAGGTGGGTATTGATACTGAATCGTTCGATGC
TGCGCTTAAAAGCTCATTGCGTCAGGCACCTGATGTTATCTTGATTGGTGAGATCCGTACTCAAGAGACCATGGAGTTTG
CACTGTCGTTTGCTGAGACGGGCCACCTTTGTATGGCAACGCTTCACGCTAACAACGCTAACCAGGCATTAGACCGTATT
ATGCACCTTGTGCCAGAGAATAAGCACAACCAGTTATTGTTCGATTTATCTTTGAACTTACGCGGTATTGTGGCGCAGCA
GCTTGTTGCTAAGTCTGACGGCAGCGGACGTCGCGCTGCGATTGAAATTTTGATTAATACGCCGCGTGTTGCCGACCTAA
TCGCTAAGAATGATCTACACTTGCTTAAAGAGACCATGGCAAAATCAAATGAGCAAGGGATGCAGACATTCGACCAAGCG
TTATTAGCCTTATATCTTGAGGGTGAAATTAGTTATGCTGATGCACTACACCATGCTGACTCACCAAATGATTTACGCTT
GATGATCAAGCTACAAAACAACGATACCTCAAGTACTGGTGGCTTTATGGAAGGTGTCACCTTAGATTTGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411PED6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.569

100

0.596
  pilU Vibrio cholerae strain A1552

57.951

100

0.58
  pilU Acinetobacter baylyi ADP1

59.375

94.879

0.563
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.443

97.305

0.394
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.727

88.949

0.38
  pilT Vibrio cholerae strain A1552

42.727

88.949

0.38
  pilT Legionella pneumophila strain Lp02

41.39

89.218

0.369
  pilT Legionella pneumophila strain ERS1305867

41.39

89.218

0.369
  pilT Acinetobacter baylyi ADP1

40.058

92.183

0.369
  pilT Acinetobacter baumannii D1279779

39.766

92.183

0.367
  pilT Acinetobacter baumannii strain A118

39.766

92.183

0.367
  pilT Acinetobacter nosocomialis M2

39.766

92.183

0.367
  pilT Pseudomonas aeruginosa PAK

38.841

92.992

0.361

Multiple sequence alignment