Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Pstu14405_RS02050 Genome accession   NZ_CP036186
Coordinates   441229..442263 (+) Length   344 a.a.
NCBI ID   WP_003282742.1    Uniprot ID   A0A2N8RHN6
Organism   Stutzerimonas stutzeri strain ATCC 14405     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 436229..447263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Pstu14405_RS02030 (Pstu14405_02025) - 436799..438766 (-) 1968 WP_036991432.1 dynamin-like GTPase family protein -
  Pstu14405_RS02035 (Pstu14405_02030) - 439038..439628 (-) 591 WP_003282747.1 YggT family protein -
  Pstu14405_RS02040 (Pstu14405_02035) proC 439638..440462 (-) 825 WP_003282745.1 pyrroline-5-carboxylate reductase -
  Pstu14405_RS02045 (Pstu14405_02040) - 440473..441162 (-) 690 WP_003282744.1 YggS family pyridoxal phosphate-dependent enzyme -
  Pstu14405_RS02050 (Pstu14405_02045) pilT 441229..442263 (+) 1035 WP_003282742.1 type IV pilus twitching motility protein PilT Machinery gene
  Pstu14405_RS02055 (Pstu14405_02050) pilU 442349..443494 (+) 1146 WP_003282740.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Pstu14405_RS02060 (Pstu14405_02055) - 443491..444327 (-) 837 WP_003282739.1 TIGR03915 family putative DNA repair protein -
  Pstu14405_RS02065 (Pstu14405_02060) - 444327..445538 (-) 1212 WP_003282738.1 putative DNA modification/repair radical SAM protein -
  Pstu14405_RS02070 (Pstu14405_02065) - 445672..446067 (+) 396 WP_003282737.1 NINE protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37916.65 Da        Isoelectric Point: 6.8360

>NTDB_id=347395 Pstu14405_RS02050 WP_003282742.1 441229..442263(+) (pilT) [Stutzerimonas stutzeri strain ATCC 14405]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVVSQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDSCL
KGLLAKGLISRDSAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=347395 Pstu14405_RS02050 WP_003282742.1 441229..442263(+) (pilT) [Stutzerimonas stutzeri strain ATCC 14405]
ATGGATATCACAGAACTGCTGGCCTTCAGCGCCAAGCAGGGTGCGTCGGATTTGCACCTTTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGGCGAATCAACCTGCCGGCCATGGACCACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAGTTTCTCGAGACTGACTTCTCGTTCGAAGTACCTGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAATCGGGGCGCCGGTGCGGTGTTCCGAACCATTCCCTCGAAAGTGCTGACCATGGA
AGATCTCGGCATGGGCGAGGTGTTTCGCAAGATCACCGACGTACCTCGGGGCTTGGTGCTGGTTACCGGGCCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCAATGCTCGATTATCTGAACAACACAAAGTACCACCACATTCTGACCATCGAAGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTCCATCGTGACACCCTGGGCTTCTCCGA
GGCGCTGCGTTCGGCACTGCGTGAAGACCCGGACATCATCCTGGTGGGCGAAATGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCTGCGGAAACCGGTCACCTGGTATTTGGCACCCTGCACACCACATCGGCCGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCCGAAGAGAAGTCCATGGTTCGCTCGATGCTCTCCGAGTCGTTGCAGGCGGTAGTCTCCCAAAC
GCTGCTGAAAAAGGTAGGCGGCGGCCGCGTGGCAGCCCACGAGATCATGATCGGTACGCCGGCGATCCGTAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATTCAGACCGGTGGTTCGCTGGGCATGCAGACGCTCGATTCGTGCCTC
AAGGGTCTGCTCGCCAAGGGGCTGATCTCGAGGGACAGCGCCAAGGAGAAGGCCAAGCAGCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N8RHN6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

99.419

100

0.994
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

83.43

100

0.834
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment