Detailed information NTG0347396

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Pstu14405_RS02055
• Genome accession: NZ_CP036186
• Coordinates: 442349..443494 (+)
• Length: 381 a.a.
• NCBI ID: WP_003282740.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri strain ATCC 14405
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 437349..448494

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Pstu14405_RS02035 (Pstu14405_02030)	-	439038..439628 (-)	591	WP_003282747.1	YggT family protein	-
Pstu14405_RS02040 (Pstu14405_02035)	proC	439638..440462 (-)	825	WP_003282745.1	pyrroline-5-carboxylate reductase	-
Pstu14405_RS02045 (Pstu14405_02040)	-	440473..441162 (-)	690	WP_003282744.1	YggS family pyridoxal phosphate-dependent enzyme	-
Pstu14405_RS02050 (Pstu14405_02045)	pilT	441229..442263 (+)	1035	WP_003282742.1	type IV pilus twitching motility protein PilT	Machinery gene
Pstu14405_RS02055 (Pstu14405_02050)	pilU	442349..443494 (+)	1146	WP_003282740.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Pstu14405_RS02060 (Pstu14405_02055)	-	443491..444327 (-)	837	WP_003282739.1	TIGR03915 family putative DNA repair protein	-
Pstu14405_RS02065 (Pstu14405_02060)	-	444327..445538 (-)	1212	WP_003282738.1	putative DNA modification/repair radical SAM protein	-
Pstu14405_RS02070 (Pstu14405_02065)	-	445672..446067 (+)	396	WP_003282737.1	NINE protein	-
Pstu14405_RS02075 (Pstu14405_02070)	-	446141..447415 (-)	1275	WP_003282736.1	dihydroorotase	-
Pstu14405_RS02080 (Pstu14405_02075)	-	447415..448431 (-)	1017	WP_003282735.1	aspartate carbamoyltransferase catalytic subunit	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42499.79 Da
• Isoelectric Point: 6.8856

>NTDB_id=347396 Pstu14405_RS02055 WP_003282740.1 442349..443494(+) (pilU) [Stutzerimonas stutzeri strain ATCC 14405]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIDELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=347396 Pstu14405_RS02055 WP_003282740.1 442349..443494(+) (pilU) [Stutzerimonas stutzeri strain ATCC 14405]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTTGAAAAGGGCGGCTCCGATCTGTTCATTACGGCCGGCGTGCCACCTTC
AATGAAGGTCAACGGCAAGATCATGCCGGTTAGCAAGACAGCGATGTCCCCGGAGATGACCCGGGAAACCGTGCATGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAATCACGAGTGCAACTTCGCCATCAGTGCCCGTGGTATCGGCCGC
TTCCGTGTCAGCGCCTTTTACCAGCGCAACCTCGCCGGAATGGTGTTGCGGCGCATCGAGACCAACATTCCCACTATCGA
TGAGCTCAAGCTGCCGGACGTCCTCAAGAAGCTGTCGATGACCAAACGCGGACTGGTGCTGTTCGTTGGCGCCACCGGTA
CCGGCAAGTCGACTTCATTGGCTTCGATGATTGGCTACCGCAACAAGAATTCCAGCGGGCACATCATTTCTATCGAGGAC
CCGATCGAATTCATCCACCAGCACCAGAGTTGCATCGTCACCCAGCGCGAAGTCGGTATCGATACCAGCTCGTTCGAGGT
AGCACTGAAGAACACCCTGCGCCAGGCGCCAGACGTGATCCTCATCGGTGAGATTCGTACCCGCGAAACCATGGACTACG
CCGTGGCGTTCGCCGAAACTGGCCACCTGTGCCTGGCGACGCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATTAACTTCTTCCCGCCTGATCGGCATAACCAGGTGTGGATGGATCTGTCCCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACCCCTGACGGCAAGGGCCGTCGTGCGGTGATCGAGGTGCTGATCAATACGCCGTTGGCGGCCGATCTGA
TTCGCAAGGGCGAGGTGCATGAACTCAAGTCGCTGATGAAGCGCTCGACCGAATTGGGCATGCAGACCTTCGATCAGGCG
CTATACAACCTCTATGTGCAGGGTGAAATCACCTACGAAGACGCGCTTTTGCATGCCGACTCGGCCAACGACCTGCGCCT
GCTGATCAAGCTGGGTTCGGAAACCGATGGTGAGCATCTGACCAGCGTGTCGCAAGGCCTGAGTCTGGAGGTCAGCGACG
AGGATCCAGGCCGCCGCTTCCGCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	94.226	100	0.942
pilU	Acinetobacter baylyi ADP1	66.387	93.701	0.622
pilU	Vibrio cholerae strain A1552	59.429	91.864	0.546
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388