Detailed information NTG0034731

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: PAU_RS16260
• Genome accession: NC_012962
• Coordinates: 3851641..3852642 (-)
• Length: 333 a.a.
• NCBI ID: WP_015835399.1
• Uniprot ID: -
• Organism: Photorhabdus asymbiotica
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3846641..3857642

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PAU_RS16230 (PAT0076)	-	3847166..3847807 (+)	642	WP_015835393.1	DsbA family protein	-
PAU_RS16235 (PAT0077)	-	3847845..3848519 (-)	675	WP_015835394.1	A24 family peptidase	-
PAU_RS16240 (PAT0078)	rsmE	3848816..3849547 (+)	732	WP_041382307.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
PAU_RS16245 (PAT0079)	gshB	3849558..3850508 (+)	951	WP_015835396.1	glutathione synthase	-
PAU_RS16250 (PAT0080)	-	3850616..3851179 (+)	564	WP_015835397.1	YqgE/AlgH family protein	-
PAU_RS16255 (PAT0081)	ruvX	3851180..3851599 (+)	420	WP_015835398.1	Holliday junction resolvase RuvX	-
PAU_RS16260 (PAT0082)	pilT	3851641..3852642 (-)	1002	WP_015835399.1	type IV pilus twitching motility protein PilT	Machinery gene
PAU_RS16265 (PAT0083)	-	3852664..3853359 (+)	696	WP_015835400.1	YggS family pyridoxal phosphate-dependent enzyme	-
PAU_RS16270 (PAT0084)	proC	3853385..3854206 (+)	822	WP_015835401.1	pyrroline-5-carboxylate reductase	-
PAU_RS16275 (PAT0085)	-	3854225..3854773 (+)	549	WP_015835402.1	YggT family protein	-
PAU_RS16280 (PAT0086)	-	3854802..3855395 (+)	594	WP_041382310.1	XTP/dITP diphosphatase	-
PAU_RS16285 (PAT0087)	hemW	3855388..3856518 (+)	1131	WP_015835404.1	radical SAM family heme chaperone HemW	-

Sequence

Protein

• Length: 333 a.a.
• Molecular weight: 36873.21 Da
• Isoelectric Point: 6.7618

>NTDB_id=34731 PAU_RS16260 WP_015835399.1 3851641..3852642(-) (pilT) [Photorhabdus asymbiotica]
MNMDKLVALSVKHNASDLHLCVGQVPVLRIDGVLYPQTQLPSINNALLEEWSRQFLSENQRQQLLTSGQVDFVLETENGQ
RLRGNFFRQQSGFSVVLRLITSQCLSLEQLKAPDIIGEMILREESGLILVTGATGSGKSTTLTAIVDRLNSYCCHHIITL
EDPVEFVHNSRNCLIQQRQIGLDSSSYQEALKGALRQDPDVLLLGELRDKETIRLALTAAETGHLVFSTLHTRGAIQTID
RLVDIFSAEEKGWVRSQLAGSLKAVIAQQLVSARGGGRIAVYEILVVNQAVSHLIREGKNHQILTLMQTGAASGMQTYEQ
GWQQRKLQGLLAE

Nucleotide

• Length: 1002 bp

>NTDB_id=34731 PAU_RS16260 WP_015835399.1 3851641..3852642(-) (pilT) [Photorhabdus asymbiotica]
ATGAATATGGATAAGTTAGTGGCCCTTAGTGTAAAGCATAATGCTTCAGATCTGCATCTTTGTGTTGGGCAGGTTCCTGT
ATTACGTATAGATGGTGTGCTTTATCCACAAACTCAACTGCCATCTATAAATAATGCATTGCTGGAAGAGTGGAGCCGGC
AGTTTTTATCAGAAAATCAGCGACAACAGCTATTAACTAGTGGACAGGTGGATTTTGTGCTGGAGACAGAAAATGGCCAA
CGATTGAGAGGAAACTTTTTTCGCCAGCAATCTGGGTTTTCAGTAGTTTTACGTTTAATTACATCTCAGTGCCTCTCTTT
GGAACAATTAAAAGCGCCTGACATTATCGGTGAAATGATTTTGCGAGAAGAAAGTGGATTGATTCTGGTAACGGGGGCTA
CAGGTAGCGGGAAATCAACAACGTTAACTGCCATTGTGGATAGGCTGAATAGTTATTGTTGTCATCACATTATTACATTG
GAAGATCCTGTGGAGTTTGTCCACAATAGTCGCAATTGCCTGATACAACAACGACAAATTGGTTTGGATTCGTCCAGCTA
TCAAGAAGCGTTGAAAGGGGCTCTCCGACAAGATCCTGATGTACTGCTGTTGGGTGAGCTACGAGATAAAGAGACAATTC
GCTTGGCGTTAACTGCGGCAGAAACGGGGCATTTAGTATTTTCAACTTTACATACCAGAGGGGCTATACAAACAATCGAC
CGGCTGGTGGATATTTTTTCAGCTGAAGAAAAAGGATGGGTACGTAGCCAATTGGCTGGGAGTTTAAAGGCTGTGATAGC
ACAGCAATTAGTCTCGGCGCGGGGAGGAGGGCGAATTGCTGTGTATGAAATATTGGTGGTTAATCAAGCGGTTAGTCATC
TGATACGGGAGGGGAAAAATCATCAGATATTAACGCTTATGCAGACAGGTGCTGCCAGTGGGATGCAAACCTATGAGCAA
GGCTGGCAGCAGCGTAAATTACAGGGGTTACTAGCAGAGTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (11-272)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.245	99.399	0.489
pilT	Vibrio cholerae strain A1552	49.245	99.399	0.489
pilT	Neisseria gonorrhoeae MS11	48.649	100	0.486
pilT	Neisseria meningitidis 8013	48.649	100	0.486
pilT	Pseudomonas stutzeri DSM 10701	47.147	100	0.471
pilT	Acinetobacter baylyi ADP1	47.13	99.399	0.468
pilT	Acinetobacter baumannii D1279779	46.988	99.7	0.468
pilT	Acinetobacter baumannii strain A118	46.988	99.7	0.468
pilT	Legionella pneumophila strain ERS1305867	48.438	96.096	0.465
pilT	Legionella pneumophila strain Lp02	48.438	96.096	0.465
pilT	Pseudomonas aeruginosa PAK	46.269	100	0.465
pilT	Acinetobacter nosocomialis M2	46.687	99.7	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.39	99.399	0.411
pilU	Pseudomonas stutzeri DSM 10701	37.576	99.099	0.372
ctsE	Campylobacter jejuni subsp. jejuni 81-176	32.8	100	0.369
pilU	Acinetobacter baylyi ADP1	37.461	96.997	0.363
pilU	Vibrio cholerae strain A1552	36.697	98.198	0.36