Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KAN02_RS14075 Genome accession   NZ_CP036171
Coordinates   2957251..2958288 (+) Length   345 a.a.
NCBI ID   WP_006581387.1    Uniprot ID   A0A0A7XL61
Organism   Acinetobacter nosocomialis strain KAN02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2952251..2963288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAN02_RS14065 (KAN02_14065) - 2955483..2956325 (-) 843 WP_004710225.1 undecaprenyl-diphosphate phosphatase -
  KAN02_RS14070 (KAN02_14070) - 2956426..2957124 (-) 699 WP_087606709.1 YggS family pyridoxal phosphate-dependent enzyme -
  KAN02_RS14075 (KAN02_14075) pilT 2957251..2958288 (+) 1038 WP_006581387.1 type IV pilus twitching motility protein PilT Machinery gene
  KAN02_RS14080 (KAN02_14080) pilU 2958316..2959434 (+) 1119 WP_002052019.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KAN02_RS14085 (KAN02_14085) fur 2959504..2959941 (-) 438 WP_002052029.1 ferric iron uptake transcriptional regulator -
  KAN02_RS14090 (KAN02_14090) - 2960053..2960451 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  KAN02_RS14095 (KAN02_14095) - 2960507..2960830 (-) 324 WP_002052062.1 RnfH family protein -
  KAN02_RS14100 (KAN02_14100) - 2960830..2961897 (-) 1068 WP_004710227.1 hypothetical protein -
  KAN02_RS14110 (KAN02_14110) - 2962050..2962505 (-) 456 WP_000782976.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38328.92 Da        Isoelectric Point: 6.7271

>NTDB_id=347297 KAN02_RS14075 WP_006581387.1 2957251..2958288(+) (pilT) [Acinetobacter nosocomialis strain KAN02]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=347297 KAN02_RS14075 WP_006581387.1 2957251..2958288(+) (pilT) [Acinetobacter nosocomialis strain KAN02]
ATGGATATTACAGAGCTACTTGCCTTCTCTGTGAAAAATGGCGCATCCGATTTGCACTTATCAGCTGGTATGCCACCAAT
GATTCGTGTCGATGGTGAAGTTCGTCGTATTAACTTGCCAGCCTTAGAACATAAAGATGTCCACCGCCTCGTTTATGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCGAATGTTGCGCGT
TTCCGTGTGAATGCATTTAACCAAAACCGTGGTGCTGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGACTAGGGCAAATCTTTAAAGATATTTGCGACTATCCTCGCGGGATTGTACTGGTCACAGGTCCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCAATGCTCGATTATATTAATGAAAACCGCTATGACCATATTTTAACGGTGGAAGAC
CCAATCGAATTTGTACACCAGTCTAAGAAATGTCTGATTAACCAGCGTGAAGTGCATCGTGATACACATGGCTTTAATGA
AGCCCTTCGTTCAGCACTACGTGAAGACCCAGATATTATTTTAGTCGGTGAGATGCGTGACCTTGAAACGATTCGATTAG
CACTTACTGCTGCCGAAACAGGTCACTTGGTATTTGGTACGCTTCACACCACATCGGCTGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCTTTACAAGCAGTTATTTCTCAGAC
CCTGCTTAAAAAGAATGGTGGTGGTCGTGTTGCGGCACATGAAATTATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCACAAATGTATTCAGCTATTCAGACTGGTGCTAACCATGGCATGACAACCCTCGACCAAAGCCTA
AAAGGTTTGGTTTCACGTGGTGTAATTAGTCCACAAACAGCCCGTACAGCGGCGAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A7XL61

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

100

100

1
  pilT Acinetobacter baumannii D1279779

99.71

100

0.997
  pilT Acinetobacter baumannii strain A118

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.721

99.71

0.835
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment