Detailed information NTG0347298

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KAN02_RS14080
• Genome accession: NZ_CP036171
• Coordinates: 2958316..2959434 (+)
• Length: 372 a.a.
• NCBI ID: WP_002052019.1
• Uniprot ID: A0A1V0YUM2
• Organism: Acinetobacter nosocomialis strain KAN02
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2953316..2964434

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KAN02_RS14065 (KAN02_14065)	-	2955483..2956325 (-)	843	WP_004710225.1	undecaprenyl-diphosphate phosphatase	-
KAN02_RS14070 (KAN02_14070)	-	2956426..2957124 (-)	699	WP_087606709.1	YggS family pyridoxal phosphate-dependent enzyme	-
KAN02_RS14075 (KAN02_14075)	pilT	2957251..2958288 (+)	1038	WP_006581387.1	type IV pilus twitching motility protein PilT	Machinery gene
KAN02_RS14080 (KAN02_14080)	pilU	2958316..2959434 (+)	1119	WP_002052019.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KAN02_RS14085 (KAN02_14085)	fur	2959504..2959941 (-)	438	WP_002052029.1	ferric iron uptake transcriptional regulator	-
KAN02_RS14090 (KAN02_14090)	-	2960053..2960451 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
KAN02_RS14095 (KAN02_14095)	-	2960507..2960830 (-)	324	WP_002052062.1	RnfH family protein	-
KAN02_RS14100 (KAN02_14100)	-	2960830..2961897 (-)	1068	WP_004710227.1	hypothetical protein	-
KAN02_RS14110 (KAN02_14110)	-	2962050..2962505 (-)	456	WP_000782976.1	bacteriohemerythrin	-
KAN02_RS14115 (KAN02_14115)	-	2962758..2963606 (-)	849	WP_009505210.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41980.18 Da
• Isoelectric Point: 6.6944

>NTDB_id=347298 KAN02_RS14080 WP_002052019.1 2958316..2959434(+) (pilU) [Acinetobacter nosocomialis strain KAN02]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPTVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLANEGPDQITDEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=347298 KAN02_RS14080 WP_002052019.1 2958316..2959434(+) (pilU) [Acinetobacter nosocomialis strain KAN02]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCCGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCAGGTGAAGTAATTGGTCAACTCTTACATTCCA
TCATGAGTGAAAAACAACGTAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCCATCATGAACCGCGATAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGTGACATGCCGGGTATGGTACTACGTCGAATTGAAACGAAAATTCCTTCAAT
TGATGACTTACAGTTGCCGACTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGTGCAACAG
GTACAGGTAAATCGACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAG
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAACGTGAAGTCGGTATTGATACAGACTCATTTGA
AATTGCTTTAAAAAATACGTTACGACAAGCGCCAGATGTCATCTTAATTGGTGAAATCCGTTCTCGCGAAGTCATGGACT
ACGCAATTGGCTTTGCTGAAACAGGACACCTTGTATTAGCTACAATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGACTTGTCTCTTAACTTAAAAGCCATGATTGCACA
GCAGCTTATTCCAACACCAGATGGTAATTCACGCCGTGCTGCGATTGAGATCTTAATTAACTCACCATTAATTTCAGACT
ATATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTGATGAAACGCTCACGTGAGCTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATACAAAGCCGGCCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTAACGATTAAACTTGCAAATGAAGGTCCTGATCAAATTACAGACGAAAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A1V0YUM2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.254	95.43	0.403