Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynWH8101_RS00095 Genome accession   NZ_CP035914
Coordinates   21617..22693 (-) Length   358 a.a.
NCBI ID   WP_130128059.1    Uniprot ID   A0AAE7J2U0
Organism   Synechococcus sp. WH 8101     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16617..27693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynWH8101_RS00070 (SynWH8101_0014) - 16967..17485 (+) 519 WP_130130263.1 RNA-binding protein -
  SynWH8101_RS00075 (SynWH8101_0015) dusA 17530..18534 (-) 1005 WP_130128055.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynWH8101_RS00080 (SynWH8101_0016) msrB 18603..19115 (+) 513 WP_130128056.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynWH8101_RS00085 (SynWH8101_0017) - 19042..20355 (+) 1314 WP_130128057.1 NAD(P)/FAD-dependent oxidoreductase -
  SynWH8101_RS00090 (SynWH8101_0018) - 20348..21604 (-) 1257 WP_130128058.1 type II secretion system F family protein -
  SynWH8101_RS00095 (SynWH8101_0019) pilT 21617..22693 (-) 1077 WP_130128059.1 type IV pilus twitching motility protein PilT Machinery gene
  SynWH8101_RS00100 (SynWH8101_0020) pilB/pilB1 22704..24341 (-) 1638 WP_130128060.1 GspE/PulE family protein Machinery gene
  SynWH8101_RS00105 (SynWH8101_0021) grpE 24450..25199 (+) 750 WP_130128061.1 nucleotide exchange factor GrpE -
  SynWH8101_RS00110 (SynWH8101_0022) dnaJ 25244..26371 (+) 1128 WP_130128062.1 molecular chaperone DnaJ -
  SynWH8101_RS00115 (SynWH8101_0023) - 26374..26616 (+) 243 WP_130128063.1 sulfurtransferase TusA family protein -
  SynWH8101_RS00120 (SynWH8101_0024) rsgA 26603..27505 (+) 903 WP_130128064.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39061.59 Da        Isoelectric Point: 6.1664

>NTDB_id=344915 SynWH8101_RS00095 WP_130128059.1 21617..22693(-) (pilT) [Synechococcus sp. WH 8101]
MELMIEDLMEQLVQGSGSDLHLASGQPPYGRFNGQLRPLSETPLSEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGNTIPSIASLELPPVVVDTCARPRGLVLVTGPTGSGKTTTLAALLDHINHSRSEHILTI
EDPIEFVYRSDRSMVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPGQQTQIRVQLSGSLVAVFAQTLCRRLDPKPGQFGRVMAQEILINTPAIANLIREGKTAQLYSQLQTGADLGM
QTLERALADLVTAGSISRDEAMAKAGKPGELDRLLGGS

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=344915 SynWH8101_RS00095 WP_130128059.1 21617..22693(-) (pilT) [Synechococcus sp. WH 8101]
ATGGAGCTGATGATCGAAGACCTGATGGAGCAGCTGGTGCAGGGCAGCGGCAGCGACCTGCATCTGGCCAGCGGGCAGCC
GCCCTACGGCCGCTTCAACGGACAGCTCCGCCCCCTCTCGGAAACGCCGCTCAGCGAAGAAAGCTGCAACCGGCTGATCT
TTTCGATGCTCAACAACAGCCAGCGCAAGACCCTCGAACAAACCTGGGAGCTGGATTGCGCCTATGGCCTCAAGGGGGTG
GCCCGCTTCCGCGTCAACGTGTATCGGCAGAAGGGGAGCTACGCCGCCTGCCTGCGGGCCCTGGGCAACACGATCCCCAG
CATCGCCTCGCTGGAGCTGCCGCCGGTGGTGGTGGACACCTGCGCCCGACCGCGAGGCCTGGTGCTGGTGACCGGACCGA
CCGGATCAGGCAAAACCACCACCCTCGCCGCCCTGCTCGACCACATCAACCACAGCCGCAGCGAACACATCCTCACGATC
GAAGACCCGATCGAATTCGTGTATCGCAGCGACCGCAGCATGGTGCACCAGCGCCAGCTCAATGAAGACACCCGCAGCTT
TGCCAATGCGCTGCGCGCCGCCTTGCGCGAAGACCCGGACGTGATCCTGGTGGGTGAAATGCGCGATCTCGAAACGATCC
AGCTGGCGATCACCGCAGCGGAAACCGGTCATCTGGTGTTTGGAACCCTGCACACCAGCTCCGCCGCCCAGACGGTGGAT
CGAATGGTGGATGTGTTCCCGCCCGGCCAGCAGACCCAGATCCGGGTGCAACTCTCCGGCAGCCTGGTGGCCGTTTTTGC
CCAGACCCTCTGCCGTCGGCTCGATCCGAAACCGGGGCAGTTCGGCCGGGTGATGGCCCAGGAGATCCTGATCAACACTC
CGGCGATCGCGAACCTGATCCGTGAAGGCAAAACCGCCCAGCTCTATTCCCAGCTGCAAACCGGTGCGGATCTGGGCATG
CAGACCCTCGAACGGGCCCTGGCCGATCTGGTGACAGCCGGCAGCATCAGCCGGGATGAGGCCATGGCCAAAGCGGGTAA
ACCGGGTGAACTGGACCGCTTGCTGGGCGGATCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.035

96.089

0.5
  pilT Pseudomonas stutzeri DSM 10701

52.035

96.089

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.857

97.765

0.497
  pilT Acinetobacter baylyi ADP1

51.163

96.089

0.492
  pilT Neisseria meningitidis 8013

51.02

95.81

0.489
  pilT Neisseria gonorrhoeae MS11

50.729

95.81

0.486
  pilT Legionella pneumophila strain ERS1305867

51.026

95.251

0.486
  pilT Legionella pneumophila strain Lp02

51.026

95.251

0.486
  pilT Acinetobacter baumannii strain A118

50.581

96.089

0.486
  pilT Acinetobacter baumannii D1279779

50.581

96.089

0.486
  pilT Acinetobacter nosocomialis M2

50.581

96.089

0.486
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

94.972

0.475
  pilT Vibrio cholerae strain A1552

50

94.972

0.475
  pilU Pseudomonas stutzeri DSM 10701

39.769

96.927

0.385
  pilU Vibrio cholerae strain A1552

38.329

96.927

0.372
  pilU Acinetobacter baylyi ADP1

37.356

97.207

0.363

Multiple sequence alignment