Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SynWH8101_RS00100 Genome accession   NZ_CP035914
Coordinates   22704..24341 (-) Length   545 a.a.
NCBI ID   WP_130128060.1    Uniprot ID   A0AAE7J0Y9
Organism   Synechococcus sp. WH 8101     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 17704..29341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynWH8101_RS00080 (SynWH8101_0016) msrB 18603..19115 (+) 513 WP_130128056.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynWH8101_RS00085 (SynWH8101_0017) - 19042..20355 (+) 1314 WP_130128057.1 NAD(P)/FAD-dependent oxidoreductase -
  SynWH8101_RS00090 (SynWH8101_0018) - 20348..21604 (-) 1257 WP_130128058.1 type II secretion system F family protein -
  SynWH8101_RS00095 (SynWH8101_0019) pilT 21617..22693 (-) 1077 WP_130128059.1 type IV pilus twitching motility protein PilT Machinery gene
  SynWH8101_RS00100 (SynWH8101_0020) pilB/pilB1 22704..24341 (-) 1638 WP_130128060.1 GspE/PulE family protein Machinery gene
  SynWH8101_RS00105 (SynWH8101_0021) grpE 24450..25199 (+) 750 WP_130128061.1 nucleotide exchange factor GrpE -
  SynWH8101_RS00110 (SynWH8101_0022) dnaJ 25244..26371 (+) 1128 WP_130128062.1 molecular chaperone DnaJ -
  SynWH8101_RS00115 (SynWH8101_0023) - 26374..26616 (+) 243 WP_130128063.1 sulfurtransferase TusA family protein -
  SynWH8101_RS00120 (SynWH8101_0024) rsgA 26603..27505 (+) 903 WP_130128064.1 ribosome small subunit-dependent GTPase A -
  SynWH8101_RS00125 (SynWH8101_0025) - 27480..27821 (-) 342 WP_130128065.1 YbaB/EbfC family nucleoid-associated protein -
  SynWH8101_RS00130 (SynWH8101_0026) murB 27854..28771 (-) 918 WP_130130264.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 545 a.a.        Molecular weight: 60036.98 Da        Isoelectric Point: 5.1194

>NTDB_id=344916 SynWH8101_RS00100 WP_130128060.1 22704..24341(-) (pilB/pilB1) [Synechococcus sp. WH 8101]
MTVHAPAAAAQNPWHQQRLELELLLQVPVPERLDAAADAQLQEAMRRKDLTQQLQPLLARRDDSRDDSHDAPAPSLLDGF
NPEGVLEEDPDENARLAGLHEDLEQSLRDSETSPVVNLVNRILLQALQLGASDIHVEPQQAGLQVRFRQDGVLQSYSEPL
PSRLVPAVTSRFKIMADLDIAERRQAQDGRIRRRFQERTVDFRVNSLPSRFGEKIVLRLLDSSATQLGLDTLITDPAALE
LVRNLGAKPFGMILVTGPTGSGKSTTLYSLLAERNDPGINISTVEDPIEYTLPGITQCQVNRDKGFDFSTALRAFMRQDP
DVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDAPSAIARLSEMGVEPFMVSASLIGIVSQRLLRKVCNHCCESYQPD
ASELGRFGLMTHNEADVRFVRAHHHDGDGQACPHCQGSGYRGRVGVYEVLRMNEELATAVARGATTDVIRRLALESGMTT
LLGYSLELVRRGQTTLEEVGRMILTDAGLESERRARALHSLTCEGCGGGLQEGWLECPYCLTPRQ

Nucleotide


Download         Length: 1638 bp        

>NTDB_id=344916 SynWH8101_RS00100 WP_130128060.1 22704..24341(-) (pilB/pilB1) [Synechococcus sp. WH 8101]
TTGACCGTCCACGCACCTGCCGCCGCTGCACAGAACCCTTGGCACCAGCAACGACTGGAGCTGGAGCTGCTGCTCCAGGT
ACCAGTACCGGAGCGTCTCGACGCTGCCGCTGATGCGCAACTGCAGGAGGCGATGCGACGGAAGGATCTGACCCAGCAGC
TGCAACCCCTCCTGGCCCGGCGCGACGACAGCCGCGACGACAGCCACGACGCCCCAGCCCCATCCCTGCTCGATGGGTTC
AACCCGGAGGGAGTTCTTGAGGAGGACCCCGACGAGAACGCCCGACTGGCAGGCCTGCACGAAGATCTGGAGCAGAGCCT
GAGGGACTCCGAAACCTCACCGGTGGTGAATCTGGTGAACCGGATCCTGCTGCAGGCCCTGCAACTGGGTGCCAGCGACA
TTCATGTGGAACCGCAGCAGGCAGGCCTGCAGGTGCGGTTCCGACAGGACGGCGTGCTGCAGAGCTACAGCGAACCGTTG
CCGAGTCGGTTGGTGCCCGCCGTGACCTCCCGCTTCAAGATCATGGCCGACCTGGACATCGCCGAACGCCGCCAGGCACA
GGACGGCCGCATCCGCAGGCGCTTCCAGGAGCGCACAGTGGATTTCCGGGTCAACAGCCTGCCAAGCCGCTTCGGCGAAA
AGATTGTGCTGCGCCTGCTCGACAGCTCCGCCACCCAGCTGGGGCTGGACACCTTGATCACCGACCCGGCGGCCCTGGAG
CTGGTGCGCAATCTCGGCGCCAAACCCTTCGGAATGATCCTGGTGACCGGGCCAACGGGCTCAGGAAAATCGACAACGCT
GTATTCGCTGCTGGCGGAGCGCAACGATCCGGGCATCAACATCTCCACCGTTGAAGATCCGATCGAATACACCCTGCCGG
GCATCACGCAGTGCCAGGTGAACCGCGACAAAGGCTTTGATTTCAGCACCGCCCTGCGCGCCTTCATGCGCCAGGACCCG
GACGTGCTGCTGGTGGGCGAAACCCGCGACCTGGAGACCGCCAAGACAGCGATCGAAGCGGCGCTCACCGGTCACCTGGT
GCTGACCACACTCCATTGCAACGATGCACCGAGTGCCATCGCCCGCCTGAGCGAGATGGGGGTGGAACCGTTCATGGTCA
GCGCCTCCCTGATCGGCATCGTGTCGCAACGCCTGCTGCGCAAGGTGTGCAACCACTGCTGCGAGAGCTATCAGCCCGAT
GCCAGCGAACTGGGGCGGTTCGGACTGATGACCCACAACGAAGCGGATGTGCGCTTTGTGCGCGCCCATCACCACGATGG
CGACGGCCAGGCCTGTCCCCACTGCCAGGGCAGTGGGTATCGGGGCCGGGTGGGGGTTTATGAAGTGCTGCGGATGAATG
AGGAGCTGGCGACGGCGGTGGCCCGTGGCGCCACCACCGACGTGATCCGGCGGCTCGCTCTGGAAAGCGGCATGACGACG
CTGCTGGGCTACAGCCTGGAACTGGTGCGCCGCGGCCAGACCACCCTGGAGGAGGTGGGCCGAATGATCCTCACCGATGC
CGGTCTGGAATCGGAACGCCGCGCCCGAGCCCTGCACAGCCTCACCTGCGAGGGCTGCGGTGGCGGACTGCAGGAAGGCT
GGCTGGAATGTCCGTACTGTCTGACCCCACGTCAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-388)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

63.077

83.486

0.527
  pilF Thermus thermophilus HB27

42.767

87.523

0.374
  pilB Vibrio cholerae strain A1552

46.905

77.064

0.361

Multiple sequence alignment