Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EPB57_RS20345 Genome accession   NZ_CP035700
Coordinates   2562044..2563150 (+) Length   368 a.a.
NCBI ID   WP_005381016.1    Uniprot ID   A0A2I3C9Z5
Organism   Vibrio alginolyticus strain 2013V-1302     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2557044..2568150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB57_RS20310 - 2557084..2557686 (-) 603 WP_005381009.1 XTP/dITP diphosphatase -
  EPB57_RS20315 - 2557805..2558236 (-) 432 WP_005381010.1 DUF4426 domain-containing protein -
  EPB57_RS20320 yggU 2558365..2558655 (-) 291 WP_005381011.1 DUF167 family protein YggU -
  EPB57_RS20325 - 2558655..2559212 (-) 558 WP_005381012.1 YggT family protein -
  EPB57_RS20330 proC 2559265..2560083 (-) 819 WP_005381013.1 pyrroline-5-carboxylate reductase -
  EPB57_RS20335 - 2560243..2560944 (-) 702 WP_154205023.1 YggS family pyridoxal phosphate-dependent enzyme -
  EPB57_RS20340 pilT 2560973..2562013 (+) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  EPB57_RS20345 pilU 2562044..2563150 (+) 1107 WP_005381016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EPB57_RS20350 ruvX 2563212..2563637 (-) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  EPB57_RS20355 - 2563673..2564236 (-) 564 WP_005381020.1 YqgE/AlgH family protein -
  EPB57_RS20360 gshB 2564364..2565314 (-) 951 WP_005381021.1 glutathione synthase -
  EPB57_RS20365 rsmE 2565328..2566059 (-) 732 WP_154205024.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EPB57_RS20370 endA 2566173..2566868 (-) 696 WP_154205025.1 deoxyribonuclease I -
  EPB57_RS20375 - 2567019..2567516 (-) 498 WP_005381025.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41254.36 Da        Isoelectric Point: 6.5943

>NTDB_id=343929 EPB57_RS20345 WP_005381016.1 2562044..2563150(+) (pilU) [Vibrio alginolyticus strain 2013V-1302]
MDLDKFLEGMLALKASDLYITVGAPILFRVDGELRPQGEKLTESDVAALLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=343929 EPB57_RS20345 WP_005381016.1 2562044..2563150(+) (pilU) [Vibrio alginolyticus strain 2013V-1302]
ATGGATCTAGATAAATTTCTCGAAGGCATGTTGGCGCTGAAAGCGTCGGATCTTTACATCACGGTTGGAGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAAAAACTCACCGAGAGTGATGTGGCTGCATTACTCGACAGCGCTA
TGGAACCAGATCGGCGTCAGGAATTTCGTAAAAGTCGCGAGTCGAATTTTGCCATTGTGAGAGATTGTGGCCGCTTCCGT
GTGAGTGCCTTTTTTCAACGCGAGTTACCAGGCGCAGTGATTCGTCGTATTGAAACTAATATTCCTACTTTTGAGCAGTT
AAAGTTACCGTTAGTGTTGCAAGATTTGGCGATAGCTAAGCGTGGTTTGGTGCTGGTGGTCGGTGCTACTGGTTCGGGTA
AATCGACCACTATGGCGGCGATGACAGGCTATCGAAACAGCAATAAAACCGGGCACATTTTGACGGTCGAAGATCCGATC
GAGTTCGTGCATGAACACAAGCGCTGTATCGTGACTCAACGTGAAGTTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGCCAAGCACCAGATATGATTTTGATTGGTGAGATCCGTAGCCGTGAAACAATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACCTGTGTATGGCAACGTTGCACGCCAACAATGCAAACCAAGCTTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTCGACTTGTCGATGAATCTGAAAGGCGTGGTTGGTCAGCAGCTGAT
CCGAGATAAAAATGGGCAGGGACGTCATGGTGTATTTGAGATCTTGCTAAATAGCCCACGTGTGTCGGATTTGATTCGTC
GCGGAGATTTACATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTTTAT
AAGTTAGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTACATAGCGCCGACTCGGCGAATGATCTGCGCTTGATGTT
GAAAACGCAGCGTGGTGAAGCATTCTCTACGGGCAGTTTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3C9Z5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.337

100

0.823
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.231

88.315

0.399
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.692

89.946

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361

Multiple sequence alignment