Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EPB57_RS20340 Genome accession   NZ_CP035700
Coordinates   2560973..2562013 (+) Length   346 a.a.
NCBI ID   WP_005381015.1    Uniprot ID   A0A2I3C9Z7
Organism   Vibrio alginolyticus strain 2013V-1302     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2555973..2567013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB57_RS20310 - 2557084..2557686 (-) 603 WP_005381009.1 XTP/dITP diphosphatase -
  EPB57_RS20315 - 2557805..2558236 (-) 432 WP_005381010.1 DUF4426 domain-containing protein -
  EPB57_RS20320 yggU 2558365..2558655 (-) 291 WP_005381011.1 DUF167 family protein YggU -
  EPB57_RS20325 - 2558655..2559212 (-) 558 WP_005381012.1 YggT family protein -
  EPB57_RS20330 proC 2559265..2560083 (-) 819 WP_005381013.1 pyrroline-5-carboxylate reductase -
  EPB57_RS20335 - 2560243..2560944 (-) 702 WP_154205023.1 YggS family pyridoxal phosphate-dependent enzyme -
  EPB57_RS20340 pilT 2560973..2562013 (+) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  EPB57_RS20345 pilU 2562044..2563150 (+) 1107 WP_005381016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EPB57_RS20350 ruvX 2563212..2563637 (-) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  EPB57_RS20355 - 2563673..2564236 (-) 564 WP_005381020.1 YqgE/AlgH family protein -
  EPB57_RS20360 gshB 2564364..2565314 (-) 951 WP_005381021.1 glutathione synthase -
  EPB57_RS20365 rsmE 2565328..2566059 (-) 732 WP_154205024.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EPB57_RS20370 endA 2566173..2566868 (-) 696 WP_154205025.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38532.02 Da        Isoelectric Point: 6.5039

>NTDB_id=343928 EPB57_RS20340 WP_005381015.1 2560973..2562013(+) (pilT) [Vibrio alginolyticus strain 2013V-1302]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVAREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=343928 EPB57_RS20340 WP_005381015.1 2560973..2562013(+) (pilT) [Vibrio alginolyticus strain 2013V-1302]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCCTCGGATCTACATCTTTCTGCAGGTGTCCCACCAAT
GGTTCGTATTGATGGGGATGTGAGAAAACTAGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTCGAAA
TCATGAACGATGCCCAACGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAGTTGCATAACGTTGGCCGA
TTCCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCAGTATTCCGTACCATTCCAAGTAGCATTCCAACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATTGCGAACGCTGAAAAAGGCTTAGTTCTGGTCACTGGCCCAACAGGTT
CAGGTAAATCGACGACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCACGTACTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAATGTTTGATCAACCAGCGTGAAGTTCACCGTGACACCCATAGTTTCCAAAA
TGCTTTGCGTAGTGCATTGCGCGAAGACCCAGACGTTATTCTGGTTGGTGAGATGCGTGACAAAGAAACCATCAGCCTTG
CCTTAACGGCGGCAGAAACGGGTCACTTAGTTTTCGGGACGTTGCACACCAGCTCGGCGGCAAAAACCATTGACCGTATT
ATCGATGTGTTTCCTGGTAGTGATAAAGATATGGTCCGCTCGATGCTATCTGAGTCCCTTCGTTCGGTTATCGCGCAAAA
GCTTCTGAAGCGTAATGGTGGTGGCCGTATTGCATGCCATGAAATTATGATGGCAACGCCTGCGATTCGTAACTTGATCC
GCGAAGACAAAGTCGCGCAGATGTATTCGATTATTCAAACAGGTGCGGCTCACGGCATGCAAACCATGGAGCAAAATGCT
CGTCAGTTAATGGCGCAAGGCATGGTTGCTCGTGAAGAGGTCGATAGTAAGATCGGATTAGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3C9Z7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.957

99.711

0.867
  pilT Vibrio cholerae strain A1552

86.957

99.711

0.867
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.429

95.087

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.399

0.671
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

65.766

96.243

0.633
  pilT Neisseria gonorrhoeae MS11

65.465

96.243

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.83

91.908

0.486
  pilU Vibrio cholerae strain A1552

41.317

96.532

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361

Multiple sequence alignment