Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ET951_RS03375 Genome accession   NZ_CP035682
Coordinates   740974..742011 (-) Length   345 a.a.
NCBI ID   WP_000350205.1    Uniprot ID   D2YE46
Organism   Vibrio mimicus strain 2011V-1073     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 735974..747011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ET951_RS03350 - 736501..737064 (+) 564 WP_154112935.1 YqgE/AlgH family protein -
  ET951_RS03355 ruvX 737132..737554 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  ET951_RS03360 tyrS 737579..738859 (-) 1281 WP_154112936.1 tyrosine--tRNA ligase -
  ET951_RS03365 - 738968..739765 (-) 798 WP_154112937.1 helix-turn-helix transcriptional regulator -
  ET951_RS03370 pilU 739853..740959 (-) 1107 WP_154112938.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ET951_RS03375 pilT 740974..742011 (-) 1038 WP_000350205.1 type IV pilus twitching motility protein PilT Machinery gene
  ET951_RS03380 - 742037..742747 (+) 711 WP_154112939.1 YggS family pyridoxal phosphate-dependent enzyme -
  ET951_RS03385 proC 742802..743620 (+) 819 WP_148559082.1 pyrroline-5-carboxylate reductase -
  ET951_RS03390 - 743678..744235 (+) 558 WP_005509546.1 YggT family protein -
  ET951_RS03395 yggU 744235..744528 (+) 294 WP_154112940.1 DUF167 family protein YggU -
  ET951_RS03400 - 744622..745052 (+) 431 Protein_655 DUF4426 domain-containing protein -
  ET951_RS03405 - 745138..745740 (+) 603 WP_000725003.1 XTP/dITP diphosphatase -
  ET951_RS03410 hemW 745734..746918 (+) 1185 WP_154112941.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38248.81 Da        Isoelectric Point: 6.5572

>NTDB_id=343590 ET951_RS03375 WP_000350205.1 740974..742011(-) (pilT) [Vibrio mimicus strain 2011V-1073]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLVFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQSRGCSAVFRTIPTVIPTLDQLEAPEIFTKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=343590 ET951_RS03375 WP_000350205.1 740974..742011(-) (pilT) [Vibrio mimicus strain 2011V-1073]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGTGAAGTTAGGAAGCTCGGCGTGCCTGCTTTTACTCATTCTGACGTACATCGCTTGGTATTTGAAA
TAATGAATGATGCCCAGCGCAGTGAATATGAAGAAAAATTAGAAGTCGATTTTTCTTTTGAATTACCCAACGTTGGCCGC
TTCCGGGTTAACGCGTTTCACCAATCGCGAGGATGCTCTGCGGTATTTCGTACCATTCCGACGGTGATACCGACGTTAGA
TCAGCTTGAAGCTCCTGAGATTTTTACCAAGATCGCCAATTATGAAAAAGGCTTAGTGCTGGTTACAGGGCCTACCGGTT
CGGGTAAATCCACCACGCTCGCGGCGATGGTCGACTACGTGAATGCTCATCATAACAAGCATATTTTGACCATTGAAGAT
CCGATTGAATTTGTACACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
CGCACTGCGTTCGGCACTGCGTGAAGACCCGGATGTGATTCTGGTTGGTGAGCTGCGTGACCAAGAAACCATTAGCTTGG
CACTCACCGCGGCAGAAACGGGTCACTTGGTGTTTGGTACTCTGCATACCAGCTCGGCGGCAAAAACCATCGACCGTATT
ATCGACGTGTTTCCAGGCAGTGATAAAGACATGGTGCGCTCAATGCTTTCTGAATCTCTGCGTGCAGTTATCGCGCAAAA
GCTCTTAAAACGCGTGGGTGGCGGTCGTGTCGCGTGCCATGAAATCATGCTGGCGACTCCGGCAATCCGTAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCAGCGCATGGTATGCAGACGATGGAGCAAAATGCG
AAGCAGCTGATTGCGCGTGGCGTAGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D2YE46

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

98.261

100

0.983
  pilT Vibrio cholerae strain A1552

98.261

100

0.983
  pilT Acinetobacter baumannii D1279779

72.024

97.391

0.701
  pilT Acinetobacter baumannii strain A118

72.024

97.391

0.701
  pilT Acinetobacter nosocomialis M2

71.726

97.391

0.699
  pilT Acinetobacter baylyi ADP1

72.121

95.652

0.69
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

66.471

98.551

0.655
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

64.242

95.652

0.614
  pilT Neisseria gonorrhoeae MS11

63.939

95.652

0.612
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.294

94.783

0.496
  pilU Vibrio cholerae strain A1552

41.916

96.812

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment