Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ET951_RS03370 Genome accession   NZ_CP035682
Coordinates   739853..740959 (-) Length   368 a.a.
NCBI ID   WP_154112938.1    Uniprot ID   -
Organism   Vibrio mimicus strain 2011V-1073     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 734853..745959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ET951_RS03345 gshB 735509..736465 (+) 957 WP_148537205.1 glutathione synthase -
  ET951_RS03350 - 736501..737064 (+) 564 WP_154112935.1 YqgE/AlgH family protein -
  ET951_RS03355 ruvX 737132..737554 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  ET951_RS03360 tyrS 737579..738859 (-) 1281 WP_154112936.1 tyrosine--tRNA ligase -
  ET951_RS03365 - 738968..739765 (-) 798 WP_154112937.1 helix-turn-helix transcriptional regulator -
  ET951_RS03370 pilU 739853..740959 (-) 1107 WP_154112938.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ET951_RS03375 pilT 740974..742011 (-) 1038 WP_000350205.1 type IV pilus twitching motility protein PilT Machinery gene
  ET951_RS03380 - 742037..742747 (+) 711 WP_154112939.1 YggS family pyridoxal phosphate-dependent enzyme -
  ET951_RS03385 proC 742802..743620 (+) 819 WP_148559082.1 pyrroline-5-carboxylate reductase -
  ET951_RS03390 - 743678..744235 (+) 558 WP_005509546.1 YggT family protein -
  ET951_RS03395 yggU 744235..744528 (+) 294 WP_154112940.1 DUF167 family protein YggU -
  ET951_RS03400 - 744622..745052 (+) 431 Protein_655 DUF4426 domain-containing protein -
  ET951_RS03405 - 745138..745740 (+) 603 WP_000725003.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41336.46 Da        Isoelectric Point: 7.3350

>NTDB_id=343589 ET951_RS03370 WP_154112938.1 739853..740959(-) (pilU) [Vibrio mimicus strain 2011V-1073]
MELNQYLDGMLSHKASDLYITVGAPILYRVDGELRAQGEVLSLANVTTLLHAMMDDARQAEFKQTREANFAVVRESGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPAVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMQYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVVGQQLLRDKNGKGRHGVFEVLLNSPRVADLIRRGDLHELKMTMARSKEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDNYASGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=343589 ET951_RS03370 WP_154112938.1 739853..740959(-) (pilU) [Vibrio mimicus strain 2011V-1073]
ATGGAGTTGAATCAATATCTGGATGGGATGCTGAGCCATAAAGCATCGGATCTTTACATCACCGTTGGAGCGCCGATTTT
ATATCGGGTCGATGGTGAATTACGCGCGCAAGGTGAAGTGCTCAGTTTGGCGAATGTGACGACCTTACTGCATGCAATGA
TGGATGACGCGAGACAAGCGGAATTTAAGCAGACGCGTGAAGCGAACTTTGCTGTAGTACGTGAAAGTGGACGTTTCCGG
GTTAGTGCGTTTTTCCAACGTGAATTGCCGGGCGCGGTGATCCGCCGGATTGAAACGCGTATTCCCACCTTTGAAGAGCT
GAAATTACCCGCGGTACTGCAAAATTTAGCGATCGCGAAACGCGGTTTAGTCTTAGTAGTGGGTGCTACGGGGTCAGGTA
AATCGACTACTATGGCAGCCATGACCGGCTATCGTAACCAACACCGTACTGGACATATCTTGACGGTAGAGGATCCGATT
GAGTTTGTGCATGAACATAAGCGCTGTATCGTCACCCAACGAGAAGTTGGCTTAGATACTGAAAGCTATGAAGTGGCGTT
GAAAAACTCGCTGCGCCAAGCACCGGATATGATTTTGATTGGTGAAATTCGCAGCCGAGAAACCATGCAATACGCGATGA
CCTTCGCGGAAACGGGGCATTTGTGTATGGCCACGCTGCACGCCAACAACGCCAACCAAGCTTTAGAGCGGATTTTGCAT
TTAGTACCGAAAGAACAGCGTGAACAGTTTTTGCTCGACCTGTCACTCAACTTAAAAGGGGTGGTTGGCCAGCAACTGTT
GCGTGATAAAAATGGCAAAGGGCGACATGGGGTGTTTGAAGTTCTGCTCAATAGCCCACGAGTCGCGGATTTGATTCGCC
GTGGCGATCTGCATGAGCTTAAAATGACGATGGCGCGTTCCAAAGAAGTAGGCATGCAGACTTTCGACCAAGCCTTGTAC
CAACTGGTGGTGGACGACAAAATCAGCGAGCAAGATGCCCTGCACAGTGCGGATTCGGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGACAACTACGCCAGCGGTTCCTTGCAGAATGTGAAGATTGATATGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

95.652

100

0.957
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.484

84.239

0.383
  pilT Acinetobacter baumannii strain A118

40.413

92.12

0.372
  pilT Acinetobacter nosocomialis M2

40.413

92.12

0.372
  pilT Acinetobacter baumannii D1279779

40.413

92.12

0.372
  pilT Pseudomonas aeruginosa PAK

40.118

92.12

0.37
  pilT Vibrio cholerae strain A1552

40.419

90.761

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.761

0.367
  pilT Acinetobacter baylyi ADP1

41.538

88.315

0.367
  pilT Pseudomonas stutzeri DSM 10701

39.528

92.12

0.364

Multiple sequence alignment