Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ETN89_RS07930 Genome accession   NZ_CP035457
Coordinates   1682246..1683280 (+) Length   344 a.a.
NCBI ID   WP_086367501.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain KC-Na-NB1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1677246..1688280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETN89_RS07895 (ETN89_07895) - 1677489..1677893 (+) 405 WP_050787742.1 DUF2884 family protein -
  ETN89_RS07900 (ETN89_07900) hemW 1677968..1679128 (-) 1161 WP_068968284.1 radical SAM family heme chaperone HemW -
  ETN89_RS07905 (ETN89_07905) - 1679128..1679721 (-) 594 WP_044174415.1 XTP/dITP diphosphatase -
  ETN89_RS07910 (ETN89_07910) yggU 1679823..1680122 (-) 300 WP_036763214.1 DUF167 family protein YggU -
  ETN89_RS07915 (ETN89_07915) - 1680119..1680676 (-) 558 WP_005297682.1 YggT family protein -
  ETN89_RS07920 (ETN89_07920) proC 1680688..1681506 (-) 819 WP_086367503.1 pyrroline-5-carboxylate reductase -
  ETN89_RS07925 (ETN89_07925) - 1681522..1682223 (-) 702 WP_086367502.1 YggS family pyridoxal phosphate-dependent enzyme -
  ETN89_RS07930 (ETN89_07930) pilT 1682246..1683280 (+) 1035 WP_086367501.1 type IV pilus twitching motility protein PilT Machinery gene
  ETN89_RS07935 (ETN89_07935) pilU 1683292..1684398 (+) 1107 WP_086367500.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ETN89_RS07940 (ETN89_07940) ruvX 1684726..1685151 (-) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  ETN89_RS07945 (ETN89_07945) - 1685148..1685711 (-) 564 WP_068945809.1 YqgE/AlgH family protein -
  ETN89_RS07950 (ETN89_07950) gshB 1685865..1686812 (-) 948 WP_005297697.1 glutathione synthase -
  ETN89_RS07955 (ETN89_07955) rsmE 1686833..1687564 (-) 732 WP_086367499.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38122.69 Da        Isoelectric Point: 6.2676

>NTDB_id=342593 ETN89_RS07930 WP_086367501.1 1682246..1683280(+) (pilT) [Photobacterium damselae subsp. damselae strain KC-Na-NB1]
MDITELLGFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPALEHSEVHRLIFDIMNDAQRRQFEEELEVDFSFELPDVGR
FRVNAFHQSRGCSAVFRTIPMTIPTLESLNVPDVFYKIAQCQRGLVLVTGATGSGKSTTIAALVDYINEHTNRHILTIED
PIEFVHHSKRCLINQREVHRDTHSFQAALRSALREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKSIDRI
IDVFPGSDKSMVRSMLSESLRAVVSQSLLKCISGGRVASHEIMMATPAIRNLIREDKIAQMYSMIQTGSAMGMQTMEQSV
KMLVAQGLVEAEEGRRIVDSAQQI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=342593 ETN89_RS07930 WP_086367501.1 1682246..1683280(+) (pilT) [Photobacterium damselae subsp. damselae strain KC-Na-NB1]
ATGGATATCACCGAATTATTAGGCTTCAGTGTAAAACATAACGCATCGGATCTGCATCTTTCAGCAGGTGTTCCACCTAT
GATCCGTGTAGATGGTGATGTACGCAAGTTAAGTTTACCTGCATTAGAACACTCAGAGGTTCATCGTCTGATTTTTGATA
TCATGAATGATGCCCAACGTCGTCAATTTGAAGAAGAGTTAGAAGTCGACTTCTCATTTGAATTACCCGATGTTGGCCGT
TTTCGTGTTAACGCCTTTCACCAATCTCGAGGTTGTTCTGCGGTCTTTCGTACTATCCCTATGACCATTCCAACGTTGGA
ATCTCTGAATGTGCCCGATGTTTTCTACAAAATAGCACAATGTCAGCGTGGATTGGTATTAGTTACAGGGGCTACAGGTT
CTGGTAAATCCACCACCATTGCAGCATTGGTTGATTATATTAATGAGCATACCAATCGTCATATCCTCACGATTGAAGAT
CCAATTGAATTTGTGCATCACAGTAAACGTTGTTTGATTAACCAACGAGAAGTGCATCGTGATACCCACAGTTTTCAAGC
CGCTTTGCGCTCGGCATTACGTGAAGATCCCGATGTGATTGTTGTGGGGGAATTGCGAGATCAAGAGACCATCAGTTTAG
CGTTAACTGCTGCAGAAACCGGACACTTGGTTTTAGGTACGCTACATACAAGTTCAGCAGCCAAAAGTATTGACCGTATT
ATCGATGTATTTCCTGGTAGTGATAAATCCATGGTTCGCTCCATGTTATCTGAGTCATTACGTGCAGTGGTTTCCCAAAG
TTTGTTGAAATGCATTAGCGGTGGTCGTGTGGCTTCCCATGAGATCATGATGGCAACCCCTGCGATTCGAAATTTGATCC
GCGAAGATAAGATTGCTCAGATGTATTCCATGATCCAAACCGGATCGGCAATGGGGATGCAGACAATGGAACAGAGTGTG
AAGATGCTGGTTGCCCAAGGACTAGTGGAAGCAGAAGAAGGTCGTCGTATTGTCGACAGTGCCCAACAGATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

79.88

96.802

0.773
  pilT Vibrio cholerae strain A1552

79.88

96.802

0.773
  pilT Acinetobacter baumannii D1279779

70.448

97.384

0.686
  pilT Acinetobacter baumannii strain A118

70.448

97.384

0.686
  pilT Pseudomonas aeruginosa PAK

70.149

97.384

0.683
  pilT Acinetobacter nosocomialis M2

70.149

97.384

0.683
  pilT Pseudomonas stutzeri DSM 10701

69.851

97.384

0.68
  pilT Acinetobacter baylyi ADP1

68.421

99.419

0.68
  pilT Legionella pneumophila strain Lp02

69.605

95.64

0.666
  pilT Legionella pneumophila strain ERS1305867

69.605

95.64

0.666
  pilT Neisseria meningitidis 8013

65.579

97.965

0.642
  pilT Neisseria gonorrhoeae MS11

65.282

97.965

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

100

0.5
  pilU Vibrio cholerae strain A1552

40.87

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

39.13

93.605

0.366

Multiple sequence alignment