Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ETN89_RS07935 Genome accession   NZ_CP035457
Coordinates   1683292..1684398 (+) Length   368 a.a.
NCBI ID   WP_086367500.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain KC-Na-NB1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1678292..1689398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETN89_RS07905 (ETN89_07905) - 1679128..1679721 (-) 594 WP_044174415.1 XTP/dITP diphosphatase -
  ETN89_RS07910 (ETN89_07910) yggU 1679823..1680122 (-) 300 WP_036763214.1 DUF167 family protein YggU -
  ETN89_RS07915 (ETN89_07915) - 1680119..1680676 (-) 558 WP_005297682.1 YggT family protein -
  ETN89_RS07920 (ETN89_07920) proC 1680688..1681506 (-) 819 WP_086367503.1 pyrroline-5-carboxylate reductase -
  ETN89_RS07925 (ETN89_07925) - 1681522..1682223 (-) 702 WP_086367502.1 YggS family pyridoxal phosphate-dependent enzyme -
  ETN89_RS07930 (ETN89_07930) pilT 1682246..1683280 (+) 1035 WP_086367501.1 type IV pilus twitching motility protein PilT Machinery gene
  ETN89_RS07935 (ETN89_07935) pilU 1683292..1684398 (+) 1107 WP_086367500.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ETN89_RS07940 (ETN89_07940) ruvX 1684726..1685151 (-) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  ETN89_RS07945 (ETN89_07945) - 1685148..1685711 (-) 564 WP_068945809.1 YqgE/AlgH family protein -
  ETN89_RS07950 (ETN89_07950) gshB 1685865..1686812 (-) 948 WP_005297697.1 glutathione synthase -
  ETN89_RS07955 (ETN89_07955) rsmE 1686833..1687564 (-) 732 WP_086367499.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ETN89_RS07960 (ETN89_07960) endA 1687696..1688400 (-) 705 WP_086367498.1 deoxyribonuclease I -
  ETN89_RS07965 (ETN89_07965) - 1688531..1689013 (-) 483 WP_086367497.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41331.47 Da        Isoelectric Point: 5.4324

>NTDB_id=342594 ETN89_RS07935 WP_086367500.1 1683292..1684398(+) (pilU) [Photobacterium damselae subsp. damselae strain KC-Na-NB1]
MKLQEILNQVVERKASDLYITVDSPCLLKVDGVLHPIGDTLDRTGVDLLFDEAMDSALYQEFVDTREANFALVRGDWRFR
VSAFWQRELPGMVVRRIETDIPDIFSLKLPMDMQKMALAKRGLVLVVGATGSGKSTSMAAMTGFRNQNRSGHILTVEDPI
EFVHKHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTQETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERQNQFLFDLSLNLKCILAQQLIPDAHGQGRHAAFELLINTPRVADLIRRGELYEIKDVMAKSGESGMMTFDQSLY
ELFTKGAITEQDALHHADSPNDLRLMIKVGSKDKTAINALDGVTVDFN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=342594 ETN89_RS07935 WP_086367500.1 1683292..1684398(+) (pilU) [Photobacterium damselae subsp. damselae strain KC-Na-NB1]
ATGAAGCTACAAGAAATTTTAAATCAGGTTGTTGAACGTAAAGCGTCGGATCTCTATATCACCGTGGATTCTCCGTGCCT
ATTAAAAGTGGATGGTGTGTTGCATCCCATTGGCGATACGTTAGATCGTACAGGGGTTGATTTACTGTTTGATGAGGCGA
TGGACAGTGCTTTATATCAAGAGTTTGTTGATACCCGAGAAGCGAATTTTGCTTTAGTTCGGGGCGATTGGCGGTTTCGT
GTCAGTGCGTTTTGGCAGCGAGAATTACCCGGCATGGTTGTTCGTCGGATTGAAACCGATATTCCAGATATCTTTAGCCT
GAAATTGCCGATGGATATGCAAAAAATGGCTCTAGCTAAACGCGGTTTAGTTTTAGTGGTTGGTGCAACGGGGTCGGGTA
AATCGACTTCGATGGCGGCAATGACCGGGTTTCGAAATCAAAATCGCAGTGGCCATATTTTAACGGTTGAAGATCCGATT
GAATTTGTCCATAAACACGATAAATGCATAGTGACTCAGCGTGAAGTTGGCCTCGATACTGAGAGTTATGAAGTGGCGCT
TAAGAACTCTTTGCGACAAGCGCCTGATATGATCTTAATCGGCGAAATTCGTACGCAAGAGACGATGGAATATGCGATGA
ATTTTGCGGAAACGGGTCATTTATGTATGGCCACATTGCACGCCAATAATGCTAACCAAGCGTTAGAGCGCATTCTGCAT
CTGGTACCGAAAGAGCGCCAAAATCAGTTTTTGTTTGATTTATCCTTGAACCTTAAATGCATTTTAGCTCAGCAACTTAT
CCCTGATGCTCATGGTCAAGGACGGCATGCTGCCTTTGAACTGTTAATTAATACACCGCGAGTGGCGGATCTTATTCGTC
GTGGTGAACTTTATGAGATCAAAGATGTGATGGCCAAATCAGGTGAATCGGGAATGATGACTTTTGATCAATCGTTGTAT
GAGTTATTCACTAAAGGTGCCATTACAGAACAAGATGCTCTTCATCATGCGGATTCTCCAAATGATTTACGTTTAATGAT
CAAAGTAGGGAGTAAAGATAAAACGGCAATCAATGCGTTGGATGGGGTAACGGTAGATTTTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.12

99.728

0.679
  pilU Pseudomonas stutzeri DSM 10701

59.777

97.283

0.582
  pilU Acinetobacter baylyi ADP1

55.211

96.467

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.691

93.207

0.389
  pilT Legionella pneumophila strain Lp02

40.299

91.033

0.367
  pilT Legionella pneumophila strain ERS1305867

40.299

91.033

0.367

Multiple sequence alignment