Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TOLA_RS04970 Genome accession   NC_012691
Coordinates   1047451..1048488 (+) Length   345 a.a.
NCBI ID   WP_012729193.1    Uniprot ID   C4LCN0
Organism   Tolumonas auensis DSM 9187     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1042451..1053488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOLA_RS04940 (Tola_0960) hemW 1043203..1044348 (-) 1146 WP_012729187.1 radical SAM family heme chaperone HemW -
  TOLA_RS04945 (Tola_0961) rdgB 1044352..1044942 (-) 591 WP_012729188.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  TOLA_RS04950 (Tola_0962) yggU 1045036..1045326 (-) 291 WP_012729189.1 DUF167 family protein YggU -
  TOLA_RS04955 (Tola_0963) - 1045326..1045877 (-) 552 WP_012729190.1 YggT family protein -
  TOLA_RS04960 (Tola_0964) proC 1045886..1046710 (-) 825 WP_012729191.1 pyrroline-5-carboxylate reductase -
  TOLA_RS04965 (Tola_0965) - 1046716..1047417 (-) 702 WP_012729192.1 YggS family pyridoxal phosphate-dependent enzyme -
  TOLA_RS04970 (Tola_0966) pilT 1047451..1048488 (+) 1038 WP_012729193.1 type IV pilus twitching motility protein PilT Machinery gene
  TOLA_RS04975 (Tola_0967) pilU 1048499..1049605 (+) 1107 WP_012729194.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TOLA_RS04980 (Tola_0968) yaaA 1049680..1050462 (+) 783 WP_012729195.1 peroxide stress protein YaaA -
  TOLA_RS04985 (Tola_0969) srmB 1050525..1051841 (-) 1317 WP_012729196.1 ATP-dependent RNA helicase SrmB -
  TOLA_RS04990 (Tola_0970) - 1052004..1052660 (+) 657 WP_218916118.1 methyltransferase -
  TOLA_RS04995 (Tola_0971) fldB 1052668..1053186 (-) 519 WP_012729198.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38341.18 Da        Isoelectric Point: 6.6929

>NTDB_id=34136 TOLA_RS04970 WP_012729193.1 1047451..1048488(+) (pilT) [Tolumonas auensis DSM 9187]
MDITELLAFGVKHNASDLHLSAGLPPMIRVDGDVRRINVPALEHRQVHSLVYDIMNDHQRKVFEEDLEVDFSFEIPNLAR
FRVNAFNQSRGVAAAFRTIPSKVLTLDELGAPPIFRDIAENPRGLVLVTGPTGSGKSTTLAAMIDYINDHYNHHIITIED
PIEFVHQSKQCLVNQREVFRDTKSFNAALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKAMVRSMLSESMRAVISQTLMKKNGGGRLAAHEIMIGIPAIRNLIREDKVAQMYSVIQTGMVHGMQTMDYCL
KNLVSRGLISVQDAKAKAVDPNSIL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=34136 TOLA_RS04970 WP_012729193.1 1047451..1048488(+) (pilT) [Tolumonas auensis DSM 9187]
ATGGATATAACCGAATTATTGGCCTTTGGTGTTAAACATAATGCATCAGACTTGCATCTGTCTGCCGGGTTGCCACCTAT
GATTCGTGTTGATGGCGATGTCCGGCGTATCAATGTACCGGCGTTAGAACATCGGCAGGTGCACAGTCTGGTATATGACA
TCATGAACGACCATCAGCGTAAGGTATTTGAAGAGGATTTGGAAGTCGACTTCTCGTTTGAGATACCGAATCTGGCTCGT
TTCCGTGTGAATGCATTTAACCAGAGCCGGGGTGTGGCCGCTGCGTTCCGTACCATTCCGAGTAAGGTGCTGACGCTTGA
TGAACTGGGCGCTCCGCCGATATTCAGAGATATTGCTGAAAATCCAAGAGGGTTGGTTCTGGTTACCGGCCCGACAGGTT
CTGGTAAATCCACCACGCTGGCAGCGATGATCGATTACATTAACGATCACTATAATCACCATATTATTACCATCGAAGAT
CCTATCGAATTCGTCCATCAGAGCAAGCAGTGTCTGGTTAACCAGCGTGAAGTGTTCCGTGATACCAAGAGCTTTAATGC
GGCACTGCGTTCAGCGTTGCGTGAAGATCCGGATATCATTCTGGTCGGTGAAATGCGAGATCTGGAAACCATTCGTCTGG
CACTGACTGCCGCGGAAACCGGTCACCTGGTGTTTGGTACTCTGCATACCACCTCTGCGGCAAAAACCATCGACCGTATT
ATCGACGTCTTCCCCGGCGAAGAAAAAGCGATGGTCCGTTCTATGTTGTCAGAATCCATGCGAGCCGTTATTTCCCAGAC
GCTGATGAAGAAAAACGGCGGCGGCCGTCTTGCTGCGCATGAGATCATGATCGGTATTCCGGCAATCCGGAACCTTATCC
GTGAAGATAAAGTGGCTCAAATGTACTCAGTTATTCAGACCGGAATGGTGCATGGTATGCAAACCATGGACTATTGTTTA
AAGAATCTGGTTTCGCGTGGTTTGATTTCTGTTCAGGACGCTAAAGCGAAGGCTGTTGATCCTAACTCCATACTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C4LCN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.841

100

0.788
  pilT Acinetobacter baumannii D1279779

78.551

100

0.786
  pilT Acinetobacter baumannii strain A118

78.551

100

0.786
  pilT Pseudomonas aeruginosa PAK

78.198

99.71

0.78
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.71

0.777
  pilT Acinetobacter baylyi ADP1

77.035

99.71

0.768
  pilT Legionella pneumophila strain Lp02

72.647

98.551

0.716
  pilT Legionella pneumophila strain ERS1305867

72.647

98.551

0.716
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.92

97.681

0.693
  pilT Vibrio cholerae strain A1552

70.92

97.681

0.693
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.478

99.42

0.522
  pilU Vibrio cholerae strain A1552

40.87

100

0.409
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.4
  pilU Acinetobacter baylyi ADP1

39.595

100

0.397

Multiple sequence alignment